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Studying Virus Replication with Fluorescence Microscopy

The results from research on SARS-CoV-2 virus replication kinetics, adaption capabilities, and cytopathology in Vero E6 cells, done with the help of fluorescence microscopy, are described in this…
Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light.

Epi-Illumination Fluorescence and Reflection-Contrast Microscopy

This article discusses the development of epi-illumination and reflection contrast for fluorescence microscopy concerning life-science applications. Much was done by the Ploem research group…
Molecular structure of the green fluorescent protein (GFP)

Introduction to Fluorescent Proteins

Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.
Neurons imaged with DIC contrast.

Differential Interference Contrast (DIC) Microscopy

This article demonstrates how differential interference contrast (DIC) can be actually better than brightfield illumination when using microscopy to image unstained biological specimens.
Image of MDCK (Madin-Darby canine kidney) cells taken with phase contrast.

Phase Contrast and Microscopy

This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.

Immersion Objectives

How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.

Precise Spatial Proteomic Information in Tissues

Despite the availability of imaging-based and mass-spectrometry-based methods for spatial proteomics, a key challenge remains connecting images with single-cell-resolution protein abundance…
Images of smooth muscle cells during wound healing. Courtesy L.S. Shankman, Ph.D., University of Virginia.

Studying Wound Healing of Smooth Muscle Cells

This article discusses how wound healing of cultured smooth muscle cells (SMCs) in multiwell plates can be reliably studied over time with less effort using a specially configured Leica inverted…

How to Prepare your Specimen for Immunofluorescence Microscopy

Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM,…

Fluorescent Dyes

A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP –…
Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imager with a 2x 0.15 NA objective at 3.4x zoom was used to produce this 425 µm Z-stack (26 positions), which is presented here as an Extended Depth of Field (EDoF) projection.  Images courtesy of Dr. Fikri Birey  from the Dr. Sergiu Pasca laboratory at Stanford University, 3165 Porter Dr., Palo Alto, CA

Download The Guide to Live Cell Imaging

In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide range of important considerations to take to…

Plant Cell Development and Morphogenesis

The article discusses how tubulin molecules, which make up microtubules, in plant cells can be studied with total internal reflection fluorescence (TIRF) microscopy to better understand tubulin…

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…

Microscopy in Virology

The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest…
Steps of Koehler Illumination

Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps

The technique of Koehler Illumination is one of the most important and fundamental techniques in achieving optimum imaging in any given light microscope set-up. Although it should be routinely used as…
Mammalian cell culure. Phase contrast and fluorescence image.

Introduction to Mammalian Cell Culture

Mammalian cell culture is one of the basic pillars of life sciences. Without the ability to grow cells in the lab, the fast progress in disciplines like cell biology, immunology, or cancer research…
Convalaria

Introduction to Widefield Microscopy

This article gives an introduction to widefield microscopy, one of the most basic and commonly used microscopy techniques. It also shows the basic differences between widefield and confocal…
Dronpa chromophore

Photoactivatable, Photoconvertible, and Photoswitchable Fluorescent Proteins

Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…
Left: Tissue cells marked with an immunolabel (FITC) illuminated with wide-band UV excitation. Note the tissue structure with blue autofluorescence. Right: Same tissue and same immunostaining with FITC label illuminated with epi-illumination using narrow-band blue (490 nm) light. Note the increased image contrast (Ploem, 1967)

Milestones in Incident Light Fluorescence Microscopy

Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
HeLa cells stimulated with LPS. Image has been subjected to deconvolution.

Chronic Inflammation Under the Microscope

In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can…
Gene editing with Cas9

Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering

The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target…
C. elegans

Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans

For scientists, technicians, and teachers working with the worm C. elegans in the research lab or classroom, this report is intended to give useful information to help improve their daly work. The aim…
Infinity port

Infinity Optical Systems

“Infinity Optics” refers to the concept of a beam path with parallel rays between the objective and the tube lens of a microscope. Flat optical components can be brought into this “Infinity Space”…

Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…

Handbook of Optical Filters for Fluorescence Microscopy

Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and…
Center a fluorescence bulb.

Video Tutorial: How to Align the Bulb of a Fluorescence Lamp Housing

The traditional light source for fluorescence excitation is a fluorescence lamp housing with mercury burner. A prerequisite for achieving bright and homogeneous excitation is the correct centering and…
Exchange a fluorescence bulb.

Video Tutorial: How to Change the Bulb of a Fluorescence Lamp Housing

When applying fluorescence microscopy in biological applications, a lamp housing with mercury burner is the most common light source. This video tutorial shows how to change the bulb of a traditional…

Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…

Basic Principles of Luminescence

There are a lot of light-emitting processes occurring in nature. Luminescence is an umbrella term for those kinds of events where light emission is not the result of high temperatures. This article…
TIRF image of brest carcinoma tumor cells expressing GFP tagged cell adhesion Molecule CD44 that is expressed on the cell membrane, imagined in TIRF.

Total Internal Reflection Fluorescence (TIRF) Microscopy

Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
Jellyfish Aequorea Victoria

Fluorescent Proteins - From the Beginnings to the Nobel Prize

Fluorescent proteins are the fundament of recent fluorescence microscopy and its modern applications. Their discovery and consequent development was one of the most exciting innovations for life…

Fluorescence Recovery after Photobleaching (FRAP) and its Offspring

FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…
Fluorescence microscope image of a life-science specimen

An Introduction to Fluorescence

This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.
Section taste buds rabbit, differential interference contrast microscope

Optical Contrast Methods

Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the…
Modulation contrast visualizes transparent, low-contrast specimens.

Integrated Modulation Contrast (IMC)

Hoffman modulation contrast has established itself as a standard for the observation of unstained, low-contrast biological specimens. The integration of the modulator in the beam path of themodern…
Transgenic Mouse Embryo, GFP

Fluorescence in Microscopy

Fluorescence microscopy is a special form of light microscopy. It uses the ability of fluorochromes to emit light after being excited with light of a certain wavelength. Proteins of interest can be…

Fields of Application

Fluorescence

Find out how fluorescence microscopes from Leica Microsystems support your research. Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its…

Micromanipulation

Witness automated, fast micromanipulation with this easy and convenient system from Leica Microsystems. Fully equipped with inverted research microscope and electronic micromanipulators, these…

Photomanipulation

The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.…

Live Cell Imaging

Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…

Virology

Do your research interests focus on viral infection and disease? Find out how you can gain insights into virology with solutions for imaging and sample preparation from Leica Microsystems.

Advanced Microscopy Techniques

Advanced microscopy techniques: Advanced microscopy techniques encompass both high-resolution and super-resolution imaging techniques. These techniques are primarily used to visualize biological…

Basic Microscopy Techniques

Basic microscopy techniques are used in instances where the entire specimen on the microscope stage is exposed to a light source. The whole specimen is illuminated by white light either from above (in…

Darkfield Microscopes

The darkfield contrast method exploits diffraction or scattering of light from structures of a biological specimen or non-uniform features of a material sample.

Phase Contrast Light Microscopes

A phase contrast light microscope offers a way to view the structures of many types of biological specimens in greater contrast without the need of stains.

DIC Microscopes

A DIC microscope is a widefield microscopy which has a polarization filter and Wollaston prism between the light source and condenser lens and also between the objective lens and camera sensor or…
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