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  • Fluorescence Microscopy

    Fluorescence Microscopy

    Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. Find out how fluorescence…
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  • Micromanipulation

    Micromanipulation

    Witness automated, fast micromanipulation with this easy and convenient system from Leica Microsystems. Fully equipped with inverted research microscope and electronic micromanipulators, these…
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  • Photomanipulation

    Photomanipulation

    The term photomanipulation encompasses a range of techniques that utilize the properties of fluorescent molecules to initiate events and observe how dynamic complexes behave over time in living cells.…
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  • Live Cell Imaging

    Live Cell Imaging

    Shifting perspective from single microscope components to a full working live cell imaging solution, Leica Microsystems integrates microscope, LAS X imaging software, cameras, and dedicated…
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  • Cell Culture

    Cell Culture

    Growing cells under lab conditions is the base for scientists working in fields of cell or developmental biology, cancer research, or any kind of life science and pharma research. Find out how Leica…
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  • Development and optimization of a differentiated airway epithelial cell model of the bovine respiratory tract
    Science Lab Topic: Widefield Microscopy

    Development and optimization of a differentiated airway epithelial cell model of the bovine respiratory tract

    Cattle are subject to economically-important respiratory tract infections by various bacterial and viral pathogens and there is an urgent need for the development of more realistic in vitro models of…
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  • Proline Metabolism is Essential for Trypanosoma brucei brucei Survival in the Tsetse Vector
    Science Lab Topic: Widefield Microscopy

    Proline Metabolism is Essential for Trypanosoma brucei brucei Survival in the Tsetse Vector

    Bloodsucking insects play a major role in the transmission of pathogens that cause major tropical diseases. Their capacity to transmit these diseases is directly associated with the availability and…
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  • mTORC1 Promotes Proliferation of Immature Schwann Cells and Myelin Growth of Differentiated Schwann Cells
    Science Lab Topic: Confocal Microscopy

    mTORC1 Promotes Proliferation of Immature Schwann Cells and Myelin Growth of Differentiated Schwann Cells

    The myelination of axons is essential for neuronal wiring and normal nervous system functions. In the peripheral nervous system, Schwann cells (SCs) form myelin sheaths around axons during nerve…
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  • Solvent immersion imprint lithography
    Science Lab Topic: Live-Cell Imaging

    Solvent immersion imprint lithography

    We expand upon our recent, fundamental report on solvent immersion imprint lithography (SIIL) and describe a semi-automated and high-performance procedure for prototyping polymer microfluidics and…
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  • Role of circadian gene Clock during differentiation of mouse pluripotent stem cells
    Science Lab Topic: CRISPR-Cas9

    Role of circadian gene Clock during differentiation of mouse pluripotent stem cells

    Biological rhythms controlled by the circadian clock are absent in embryonic stem cells (ESCs). However, they start to develop during the differentiation of pluripotent ESCs to downstream cells.…
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  • KChIP2 regulates the cardiac Ca²⁺ transient and myocyte contractility by targeting ryanodine receptor activity
    Science Lab Topic: Confocal Microscopy

    KChIP2 regulates the cardiac Ca²⁺ transient and myocyte contractility by targeting ryanodine receptor activity

    Pathologic electrical remodeling and attenuated cardiac contractility are featured characteristics of heart failure. Coinciding with these remodeling events is a loss of the K+ channel interacting…
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  • Effect of curcumin analogs onα-synuclein aggregation and cytotoxicity
    Science Lab Topic: Widefield Microscopy

    Effect of curcumin analogs onα-synuclein aggregation and cytotoxicity

    Alpha-synuclein (α-Syn) aggregation into oligomers and fibrils is associated with dopaminergic neuron loss occurring in Parkinson’s disease (PD) pathogenesis. Compounds that modulate α-Syn aggregation…
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  • Expression Analysis of Platelet‐derived Growth Factor Receptor Alpha and its Ligands in the Developing Mouse Lung
    Science Lab Topic: Widefield Microscopy

    Expression Analysis of Platelet‐derived Growth Factor Receptor Alpha and its Ligands in the Developing Mouse Lung

    Activation of the platelet‐derived growth factor receptor‐α (PDGFR α) signaling pathway is critically important during lung alveogenesis, the process in lung development during which alveoli are…
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  • Acute Transcriptional Up-regulation Specific to Osteoblasts/Osteoclasts in Medaka Fish Immediately after Exposure to Microgravity
    Science Lab Topic: Fluorescence Microscopy

    Acute Transcriptional Up-regulation Specific to Osteoblasts/Osteoclasts in Medaka Fish Immediately after Exposure to Microgravity

    Bone loss is a serious problem in spaceflight; however, the initial action of microgravity has not been identified. To examine this action, we performed live-imaging of animals during a space mission…
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  • Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells
    Science Lab Topic: Widefield Microscopy

    Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells

    Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy…
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  • Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps
    Science Lab Topic: Basics in Microscopy

    Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps

    The technique of Koehler Illumination is one of the most important and fundamental techniques in achieving optimum imaging in any given light microscope set-up. Although it should be routinely used as…
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  • Introduction to Mammalian Cell Culture
    Science Lab Topic: Live-Cell Imaging

    Introduction to Mammalian Cell Culture

    Mammalian cell culture is one of the basic pillars of life sciences. Without the ability to grow cells in the lab, the fast progress in disciplines like cell biology, immunology, or cancer research…
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  • Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution
    Science Lab Topic: Basics in Microscopy

    Immersion Objectives: Using Oil, Glycerol, or Water to Overcome some of the Limits of Resolution

    To examine specimens at high magnifications using the microscope, there are a number of factors which need to be taken into consideration. These include resolution, numerical aperture (NA), the…
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  • Graphene-Based Microbots for Toxic Heavy Metal Removal and Recovery from Water
    Science Lab Topic: Basics in Microscopy

    Graphene-Based Microbots for Toxic Heavy Metal Removal and Recovery from Water

    Heavy metal contamination in water is a serious risk to the public health and other life forms on earth. Current research in nanotechnology is developing new nanosystems and nanomaterials for the fast…
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  • Introduction to Widefield Microscopy
    Science Lab Topic: Widefield Microscopy

    Introduction to Widefield Microscopy

    One of the most basic microscopy techniques is known as ‘Widefield Microscopy’. It is fundamentally any technique in which the entire specimen of interest is exposed to the light source with the…
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  • Coming to Grips with Biological Information Through Flexible Organic Electronics: Developing Bendable and Stretchable Biosensors and Device
    Science Lab Topic: Stereo Microscopy

    Coming to Grips with Biological Information Through Flexible Organic Electronics: Developing Bendable and Stretchable Biosensors and Device

    What do you associate with the word sensor? Perhaps technologies delivering automation in factories and other production sites? That may be what comes to mind, but advances in organic electronics are…
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  • What is Photomanipulation?
    Science Lab Topic: Fluorescence Microscopy

    What is Photomanipulation?

    The term photomanipulation describes a wide range of techniques that enable the microscopist the transition from passive observer to instigator of events by offering a way of interacting with their…
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  • Webinar On-Demand: Quality with Confidence
    Science Lab Topic: Digital Microscopy

    Webinar On-Demand: Quality with Confidence

    From routine quality assurance to advanced 3D analysis of complex surface structures, there is a digital microscope solution to meet almost any request
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  • Photoactivatable, photoconvertible, and photoswitchable Fluorescent Proteins
    Science Lab Topic: Fluorescence Microscopy

    Photoactivatable, photoconvertible, and photoswitchable Fluorescent Proteins

    Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…
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  • Clap On, Clap Off. Protein On, Protein Off
    Science Lab Topic: Neuroscience

    Clap On, Clap Off. Protein On, Protein Off

    Unlike genetic or pharmacological manipulation, light-controlled proteins respond immediately, can be temporally and spatially triggered, are reversible, and are specific to the protein of interest.…
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  • Milestones in Incident Light Fluorescence Microscopy
    Science Lab Topic: Basics in Microscopy

    Milestones in Incident Light Fluorescence Microscopy

    Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
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  • The Molecular Architecture of Hemidesmosomes as Revealed by Super-Resolution Microscopy
    Science Lab Topic: Super-Resolution

    The Molecular Architecture of Hemidesmosomes as Revealed by Super-Resolution Microscopy

    Hemidesmosomes have been extensively studied by immunofluorescence microscopy, but due to its limited resolution, their precise organization remained poorly understood. We studied hemidesmosome…
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  • Chronic Inflammation Under the Microscope
    Science Lab Topic: Fluorescence Microscopy

    Chronic Inflammation Under the Microscope

    In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can…
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  • TIRF Publication List
    Science Lab Topic: TIRF Microscopy

    TIRF Publication List

    This monthly updated references list presents current papers using Leica AM TIRF in the major application fields for TIRF microscopy.
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  • Factors to Consider When Selecting a Research Microscope
    Science Lab Topic: Basics in Microscopy

    Factors to Consider When Selecting a Research Microscope

    An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
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  • Microscope Resolution: Concepts, Factors and Calculation
    Science Lab Topic: Basics in Microscopy

    Microscope Resolution: Concepts, Factors and Calculation

    In microscopy, the term ‘resolution’ is used to describe the ability of a microscope to distinguish detail. In other words, this is the minimum distance at which two distinct points of a specimen can…
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  • Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering
    Science Lab Topic: Fluorescence Microscopy

    Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering

    The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target…
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  • Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex
    Science Lab Topic: Super-Resolution

    Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex

    Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively investigated, the…
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  • Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles
    Science Lab Topic: Super-Resolution

    Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles

    Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been…
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  • Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans
    Science Lab Topic: Stereo Microscopy

    Work Efficiently in Developmental Biology with Stereo and Confocal Microscopy: C. elegans

    For scientists, technicians, and teachers working with the worm C. elegans in the research lab or classroom, this report is intended to give useful information to help improve their daly work. The aim…
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  • Infinity Optical Systems
    Science Lab Topic: Widefield Microscopy

    Infinity Optical Systems

    “Infinity Optics” refers to the concept of a beam path with parallel rays between the objective and the tube lens of a microscope. Flat optical components can be brought into this “Infinity Space”…
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  • CRISPR/Cas9-mediated Endogenous Protein Tagging for RESOLFT Super-Resolution Microscopy of Living Human Cells
    Science Lab Topic: Super-Resolution

    CRISPR/Cas9-mediated Endogenous Protein Tagging for RESOLFT Super-Resolution Microscopy of Living Human Cells

    Overexpression is a notorious concern in conventional and especially in super-resolution fluorescence light microscopy studies because it may cause numerous artifacts including ectopic sub-cellular…
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  • iMSRC: Converting a Standard Automated Microscope into an Intelligent Screening Platform
    Science Lab Topic: Confocal Microscopy

    iMSRC: Converting a Standard Automated Microscope into an Intelligent Screening Platform

    Microscopy in the context of biomedical research is demanding new tools to automatically detect and capture objects of interest. The few extant packages addressing this need, however, have enjoyed…
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  • How to Prepare Your Specimen for Immunofluorescence Microscopy
    Science Lab Topic: Fluorescence Microscopy

    How to Prepare Your Specimen for Immunofluorescence Microscopy

    Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM,…
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  • Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy
    Science Lab Topic: TIRF Microscopy

    Video Talk by Daniel Axelrod: Total Internal Reflection Fluorescence (TIRF) Microscopy

    Total Internal Reflection Fluorescence (TIRF) Microscopy is a technique that only illuminates dye molecules near a surface. In this video, the pioneer of TIRF Microscopy describes what this technique…
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  • Immunotherapy to Combat Cancer: "Sleeping Beauty" – DNA Plasmid-based Gene Transfer System to Modify T Cells
    Science Lab Topic: Live-Cell Imaging

    Immunotherapy to Combat Cancer: "Sleeping Beauty" – DNA Plasmid-based Gene Transfer System to Modify T Cells

    Fighting cancer is a major goal of present-day medicine. So far mainly surgery, chemotherapy or radiation therapy are utilized to extinguish cancerous tissue, or at least set limits to it. …
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  • Finding, Defining and Breaking the Diffraction Barrier in Microscopy – A Historical Perspective
    Science Lab Topic: Super-Resolution

    Finding, Defining and Breaking the Diffraction Barrier in Microscopy – A Historical Perspective

    Diffraction plays a crucial role in microscopy as it prevents the recording of arbitrarily sharp images with conventional light microscopes. Many names are connected with the notion of diffraction and…
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  • Video Talk by Karl Deisseroth: Optogenetics
    Science Lab Topic: Neuroscience

    Video Talk by Karl Deisseroth: Optogenetics

    Optogenetics is a combination of genetics and optics to achieve a gain or loss of function of biochemical events such as action potentials in a particular neuron or tissue. Opsin genes encode proteins…
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  • Optogenetic Toolkit Goes Multicolor
    Science Lab Topic: Neuroscience

    Optogenetic Toolkit Goes Multicolor

    Optogenetics is a technique that allows scientists to control neurons’ electrical activity with light by engineering them to express light-sensitive proteins. Within the past decade, it has become a…
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  • Step by Step Guide to Fluorescence Microscopy
    Science Lab Topic: Fluorescence Microscopy

    Step by Step Guide to Fluorescence Microscopy

    Fluorescence Microscopy is a special form of light microscopy. It uses fluorescence to highlight structures in fixed and living biological specimens instead of using absorption, phase or interference…
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  • Optogenetics
    Science Lab Topic: Neuroscience

    Optogenetics

    Optogenetics is a technique that allows light-controlled responses of transfected cells. The cells are genetically modified by introduction of genes that code for light-induced channels or ion pumps.…
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  • Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results
    Science Lab Topic: TIRF Microscopy

    Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

    The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…
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  • Basic Principles of Luminescence
    Science Lab Topic: Fluorescence Microscopy

    Basic Principles of Luminescence

    There are a lot of light-emitting processes occurring in nature. Luminescence is an umbrella term for those kinds of events where light emission is not the result of high temperatures. This article…
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  • Protein Transport Processes at the Apical Membrane of Polarized Epithelial Cells
    Science Lab Topic: TIRF Microscopy

    Protein Transport Processes at the Apical Membrane of Polarized Epithelial Cells

    Due to their special role in organ function and the exchange of biological components some body cells developed certain polarization characteristics. These are reflected in differences of their plasma…
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  • Total Internal Reflection Fluorescence (TIRF) Microscopy
    Science Lab Topic: TIRF Microscopy

    Total Internal Reflection Fluorescence (TIRF) Microscopy

    Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…
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  • Live-cell Imaging Techniques
    Science Lab Topic: Live-Cell Imaging

    Live-cell Imaging Techniques

    The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focusing on dynamic…
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  • Fluorescence Recovery after Photobleaching (FRAP) and its Offspring
    Science Lab Topic: Confocal Microscopy

    Fluorescence Recovery after Photobleaching (FRAP) and its Offspring

    FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…
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  • Optical Contrast Methods
    Science Lab Topic: Basics in Microscopy

    Optical Contrast Methods

    Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the…
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  • Fluorescence in Microscopy
    Science Lab Topic: Fluorescence Microscopy

    Fluorescence in Microscopy

    Fluorescence microscopy is a special form of light microscopy. It uses the ability of fluorochromes to emit light after being excited with light of a certain wavelength. Proteins of interest can be…
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  • TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells
    Science Lab Topic: Live-Cell Imaging

    TIRF Microscopy of the Apical Membrane of Polarized Epithelial Cells

    Application of TIRF microscopy (Total Internal Reflection Fluorescence) allows the visualization of structures at the apical surface of polarized epithelial cells that have been hidden in conventional…
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  • Exploring Cell Logistics
    Science Lab Topic: Live-Cell Imaging

    Exploring Cell Logistics

    Using TIRF microscopy, scientists have been able to take a closer look at intracellular transport processes with the example of the galactose-binding protein Galectin-3, which has been identified as a…
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