Leica LMD Systems Laser Microdissection Microscopes

Dissection Perfection

Dedicated Laser Microdissection Objectives

Leica Microsystems offers a portfolio of dry objectives dedicated for Leica LMD systems. These special LMD objectives feature the highest possible UV-transmission and outstanding imaging performance – the Leica SmartCut series (5x–150x).

ObjectiveMag.NAWD (mm)BF, POLFLDIC, PHLMDCISOV
HCX PL FLUOTAR**1.25x0.043.7+++++
PLAN**4x0.126.2+++++++
UVI5x0.1211.7+++++++++
HI PLAN6.3x0.1312.8+++++++++
HCX PL FLUOTAR10x0.311.0++++++++
UVI10x0.252.9+++++++
HCX PL FLUOTAR20x0.46.9++++++++
HCX PL FLUOTAR40x0.63.3–1.9++++++
HCX PL FLUOTAR63x0.72.6–1.8++++++
HCX PL FLUOTAR150x0.90.25++++++

Mag. – magnification; NA – numerical aperture; WD – working distance; BF – brightfield; POL – polarized light; FL – fluorescence; DIC – differential interference contrast; PH – phase contrast; LMD – laser microdissection; CI – cap inspection; SOV – specimen overview; + = suitable; – = not suitable; ++ = dedicated for; +++ = most suitable

** Additionally recommended objectives

Technical Specifications

DissectionDissection and Collection Unit Based on Scanning StageDissection and Collection Unit Based on Motorized StageDissection and Collection Unit Based on Scanning Stage LMT350
Specimen CollectionContact- and contamination-free
Dissection modesDraw & Cut
Move & Cut (direct online cutting)
PtoP (point-to-point)
Draw & Scan (dot dissection scan)
LMD screw (cut subsequently in Z-direction)
Shadow Cuts (parallel draw and dissection)
Serial section cuttingYesNoYes
AVC+ basic
AVC+ professional
Yes
Yes
Yes
Not recommended
Yes
Universal holderYesNoNo
Stage repeatability+/- 1 µm+/- 10 µm<=+/- 0.5 µm
Stage max. travel speed20 mm/s200 mm/s
Stage driveBall bearing screwRack/ pinionLinear motor drives
Holders3x standard slides (25 mm x 76 mm)
Optional:
1x big slide (50 mm x 76 mm) or 2x standard slides (25 mm x 76 mm)
Petri dish (50 mm)
18-well slide (stack)
1x standard slide (25 mm x 76 mm)
Optional:
1x big slide (50 mm x 76 mm) or 2x standard slides (25 mm x 76 mm)
Petri dish (50 mm)
4x standard slides (25 mm x 76 mm) Optional (combinations possible): 1x big slide (50 mm x 76 mm) 2x Petri dishes (50 mm) 2x 18-well Ibidi slide stacks
Collectors4x 0.2 ml standard PCR tubes
4x 0.5 ml standard PCR tubes
Petri dish (50 mm)
Optional:
2x 8-well strips building up a 96-well plate
Height adjustable universal collector (for multi-well slides, 8-strips, 8-strip tube caps, ...)
48-well collector (6x8-strip tube caps) (2x for a full 96-well plate)
4x 0.2 ml standard PCR tubes
4x 0.5 ml standard PCR tubes
Optional:
1x 8-well strips building up a 96-well plate
4x 0.2 ml standard PCR tubes 4x 0.5 ml standard PCR tubes 2x Petri dishes (50 mm) Optional: 96-PCR-well plate (non-skirted or semi-skirted) 96-LCC-well plate 2x 18-well Ibidi slides 1 x 8-strip, 1 x 12-strip 1.5 ml tube (with or without filling)
Power supplyCTR6/CTR6 LEDDual SP box
Laser

Leica LMD7

Leica LMD6

TypeDiode pumped, solid stateDiode pumped, solid state
Wavelength349 nm355 nm
Average pulse energy120 µJ70 µJ
Repetition rate10 – 5000 Hz (free adjustable)80 Hz (fix)
Laser aperture controlYes, continuously adjustable
Free intensity control1-100%
UV offset freely adjustable and saved for each objectiveYes
Laser beam movementVia optics
Microscope stand (fully automated DM6 B)
Transmitted light (TL) axisContrast methodsBF, optional PH, DF, POL, DIC (fully automated)
IlluminationLED (PMax = 15 W ) Lamphousing
AutomationAutomated illumination manager
Automated contrast manager
Constant color intensity control (CCIC) for Halogen TL
CondenserCondenser head S28, 0.55 NA Motorized 7x condenser disk
Motorized polarizer
Fluorescence axisFilter cube turretMotorized 5x or 8x
AutomationFluorescence intensity manager (FIM) for brightness adjustment
Circular and rectangular field diaphragms for eyepiece or camera viewing
Internal filter wheel and motorized Excitation Manager
IlluminationLeica EL6000 (120 W metal halide) or
100 W HBO
Cubes
(individual filters available upon request)
Special cubes (size k) for simultaneous fluorescence and laser application
LMD-BGR
LMD-GFP band pass LMD-GFP long pass LMD-Cy3
LMD-DAPI
LMD-Alexa594
LMD-CFP
LMD-GFP/Cy3
LMD-YFP LMD-Cy5
OperationFocusMotorized:
– 5 electronic ratios
– Includes parfocal function
Memory function for two z-positions
Objective turretMotorized 7x M25 thread including dry and immersion modes
Controls6 programmable function buttons
Leica SmartMove
Controls for z (focus) movement and x, y (stage) movement
4 programmable function buttons
Optional Leica STP8000
Controls for z (coarse and fine focus) and x, y (stage) movement
11 programmable function buttons
Touchscreen with information and control panels
StandDisplayWith integrated touchscreen Leica SmartTouch
Interfaces2 x USB 2.0, 2 x I2C
DimensionsWith scanning stage:
649.6 mm height, 512.0 mm width, 596.5 mm depth
System software (Operating System Microsoft® Windows® 7)
Package includes
(selected features)
DissectionAutomated collection devices and positioning of PCR tubes
Fully automated inspection mode
Multi-cutting over entire slide Save and load drawn shapes
User guidanceWorkflow based graphical user interface
Free scaling of drawn shapes
Save user profiles
Overview images in BF and Fluorescence
ControlFull laser control
Control software of microscope stand
Laser and illumination settings are linked to objectives
InterfacesExport shape list data for Microsoft Excel or OpenOffice
Optional software packages Automated vision control (AVC+ basic) for automated cell recognition within field of view only Automated vision control (AVC+ professional) for fully or semi automated cell recognition over freely defined area
Integrated database to transfer relevant data (laser, microscope and camera; database as optional)

Popular Configurations

Effective April 7, 2025, Leica Microsystems transactions will apply an adjustment tariff charge due to new U.S. tariffs on all applicable products.
EP-DIRECTOR LMD-Slides, 50 slides

EP-DIRECTOR LMD-Slides, 50 slides

Director slides for direct ablation of material with the LMD, pack of 50 slides

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FLUO FrameSlide, glass-like, 5 pcs

FLUO FrameSlide, glass-like, 5 pcs

Metal frame slides for FLUO, glass like membrane, pack of 5 slides

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pE 300 lite SB direct fit

pE 300 lite SB direct fit

pE 300 lite SB direct fit

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PEN Frame Slide(4µm),steel frames,50pcs.

PEN Frame Slide(4µm),steel frames,50pcs.

Metal frame slides with a 4 µm thick PEN membrane, pack of 50

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PEN Membrane Slide(4µm),glass,50 slides

PEN Membrane Slide(4µm),glass,50 slides

Glass slides with a 4 µm thick PEN membrane, pack of 50

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PEN MembranSlide, glass, 50 slides

PEN MembranSlide, glass, 50 slides

For laser microdissection special membranes mounted to a glass slide are used to achieve highest quality results. Glass slides covered with a membrane with high UV absorption, making them particularly suitable for cold photo ablation (vaporization), are called glass membrane slides. Applied to the upper surface of the glass slide, the membrane is cemented to the glass at its edges. Thus, a rectangular area to mount the specimen is created where air is between the membrane and the glass backbone. The membrane is specially coated to ensure optimal adhesion of the specimens. In general, glass membrane slides have versatile application potential. Careful treatment of the foils is essential to avoid damage to the membrane. The accumulation of moisture between the glass and the membrane should be avoided, as this can cause the dissectates to stick to the glass backbone.

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PEN MembranSlide, glass, RNase-free,50pc

PEN MembranSlide, glass, RNase-free,50pc

Glass slides with a 2 µm thick PEN membrane for RNA work, Rnase-free, pack of 50

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PEN MembranSlides,glass,large,4x25 pcs

PEN MembranSlides,glass,large,4x25 pcs

Large glass slides (50x76 mm) with a 2 µm thick PEN membrane, 4 packs of 25, 100 total slides

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PEN Petri Dishes 4 µm (1 pack=20 pc)

PEN Petri Dishes 4 µm (1 pack=20 pc)

Petri dishes with 4 µm thick PEN membrane bottom for cutting, pack of 20

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PEN WhiteCoat(2µm), glass, 200 pcs

PEN WhiteCoat(2µm), glass, 200 pcs

Glass slides with a 2 µm thick PEN membrane, slide has writable area on one end, pack of 200

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PET FrameSlide, steel frames, 50 slides

PET FrameSlide, steel frames, 50 slides

PET frame slide, a pack of 50 steel frames which can also be used as sample carriers. Leica Microsystems consumables are designed to deliver reliable performance across demanding research workflows. Leica LMD slides and immersion oils work together to support precision, reproducibility, and confidence in laser microdissection and high‑resolution imaging applications. Leica LMD slides are available in multiple membrane types and formats, allowing researchers to select the optimal slide for their sample, detection method, and experimental goal. This flexibility supports both routine workflows and advanced applications such as fluorescence, while demo slide kits make it easy to compare options and identify the best fit.

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PET FrameSlide,steel frames, RNase-free

PET FrameSlide,steel frames, RNase-free

Metal frame slides with a 1.4 µm thick PET membrane for RNA work, RNase-free, pack of 50

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POL FrameSlide, steel frames, 50 slides

POL FrameSlide, steel frames, 50 slides

Metal frame slides with a 0.9 µm thick POL membrane, pack of 50

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POL FrameSlide, steel, 50pc RNase-free

POL FrameSlide, steel, 50pc RNase-free

Metal frame slides with a 0.9 µm thick POL membrane for RNA work, RNase-free, pack of 50

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PPS FrameSlide (4 µm), steel frames, 50

PPS FrameSlide (4 µm), steel frames, 50

Metal frame slides with a 4 µm thick PPS membrane, pack of 50

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pre-printed LMD Frame Slides

pre-printed LMD Frame Slides

Metal frame slides with a 4 µm thick PEN membrane with markings for automated shape imports, 10 packs of 5 slides, 50 slides total

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pre-printed LMD Membrane Slides

pre-printed LMD Membrane Slides

Glass slides with a 4 µm thick PEN membrane with markings for automated shape imports, 10 packs of 5 slides, 50 slides total

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µ-Slide w. membrane, 18well,ibidi,15 pcs

µ-Slide w. membrane, 18well,ibidi,15 pcs

18-well Ibidi u-slide with 2 µm PEN membrane bottom, pack of 15

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