Image Gallery: THUNDER Imager
To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve this using Computational Clearing our new opto-digital technology. The result is high-speed, high-quality imaging of a large diversity of three-dimensional samples, including model organisms, tissue sections, and 3D cell cultures. Take a look at these images to see how THUNDER Imagers are already helping researchers to reveal the finest structural details even deep within a sample. Once you have seen the haze-free image quality achieved with THUNDER Imagers, find out more on our product page. Learn how THUNDER Imagers combine Computational Clearing with the speed, fluorescence-signal sensitivity, and ease-of-use of widefield microscopes so you can decode 3D biology in real time*.
*in accordance with ISO/IEC 2382:2015
A part of the work at the Keppler lab involves the use of pre-clinical models for inflammatory disease to understand the interplay between cells during inflammation. In order to understand the distribution of the cells within the organ, the lab researchers needed to change from imaging 2D cryo-sections of lymph nodes to a method that is capable of viewing the whole organ.
They developed a clearing protocol to prepare the lymph nodes for imaging. Initially, they performed the imaging experiments using a confocal microscope, but this way took up to 18 hours to image just half of a lymph node. They could not use traditional widefield systems, because the autofluorescence coming from the tissue makes it impossible to see any significant amount of the desired signal.
Only with a THUNDER Imager, we could consider using a widefield-based system to study our organs
Using a THUNDER Imager, the Keppler lab researchers found that they could clearly image the cells of interest within the lymph node in only a few minutes. In the example shown, they were able to clearly study the distribution of follicular cells (yellow), and blood vessels (magenta). Then they went on to study differences in wild type vs. inflamed lymph nodes. Having this initial overview in just 15 minutes helped them develop a more efficient screening workflow where they could then select the areas they wanted to analyze in detail using confocal microscopy enabling more detail segmentation or single cell analysis.
The THUNDER Imager has made our research more efficient, in terms of time savings, and more complete, as we can now look at whole structures and are not limited at just looking at parts of the organ