Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture.
Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture.
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Image Gallery: THUNDER Imager

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve this using Computational Clearing our new opto-digital technology. The result is high-speed, high-quality imaging of a large diversity of three-dimensional samples, including model organisms, tissue sections, and 3D cell cultures. Take a look at these images to see how THUNDER Imagers are already helping researchers to reveal the finest structural details even deep within a sample.   Once you have seen the haze-free image quality achieved with THUNDER Imagers, find out more on our product page. Learn how THUNDER Imagers combine Computational Clearing with the speed, fluorescence-signal sensitivity, and ease-of-use of widefield microscopes so you can decode 3D biology in real time*.

*in accordance with ISO/IEC 2382:2015

Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture.

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Mouse lymphnode - Getting the complete picture for inflammation research

A part of the work at the Keppler lab involves the use of pre-clinical models for inflammatory disease to understand the interplay between cells during inflammation. In order to understand the distribution of the cells within the organ, the lab researchers needed to change from imaging 2D cryo-sections of lymph nodes to a method that is capable of viewing the whole organ.

They developed a clearing protocol to prepare the lymph nodes for imaging. Initially, they performed the imaging experiments using a confocal microscope, but this way took up to 18 hours to image just half of a lymph node. They could not use traditional widefield systems, because the autofluorescence coming from the tissue makes it impossible to see any significant amount of the desired signal.

Only with a THUNDER Imager, we could consider using a widefield-based system to study our organs

Using a THUNDER Imager, the Keppler lab researchers found that they could clearly image the cells of interest within the lymph node in only a few minutes. In the example shown, they were able to clearly study the distribution of follicular cells (yellow), and blood vessels (magenta). Then they went on to study differences in wild type vs. inflamed lymph nodes. Having this initial overview in just 15 minutes helped them develop a more efficient screening workflow where they could then select the areas they wanted to analyze in detail using confocal microscopy enabling more detail segmentation or single cell analysis.

The THUNDER Imager has made our research more efficient, in terms of time savings, and more complete, as we can now look at whole structures and are not limited at just looking at parts of the organ

Arabidopsis thaliana root - THUNDER Imager

Hela Cells - THUNDER Imager

Mouse brain cortex - THUNDER Imager

Mouse embryonic kidney - THUNDER Imager

Murine esophageal organoids - THUNDER Imager

Adult rat brain - THUNDER Imager

Mouse whole mount retina - THUNDER Imager

1 mm mouse brain section - THUNDER Imager

C. elegans. Transgenic GFP - THUNDER Imager

Mouse cortical neurons - THUNDER Imager

Adult Drosophila muscle - THUNDER Imager

Drosophila Embryo - THUNDER Imager

Mouse heart - THUNDER Imager 3D Cell Culture

Drosophila Follicles - THUNDER Imager 3D Cell Culture

Cortical neurons - THUNDER Imager

Microglia in hippocampal region - THUNDER Imager

Organoid Cluster - THUNDER Imager

Cranial nerves development - THUNDER Imager 3D Cell Culture

Pancreatic islet - THUNDER Imager 3D Cell Culture

Mouse Brain - THUNDER Imager

Pollen Flower - THUNDER Imager

C. elegans Gonades - THUNDER Imager

Mouse aorta - THUNDER Imager 3D Live Cell

Mouse Dystrophin staining on muscle fibres - THUNDER Imager

Infected MDCK epithelial cells - THUNDER Imager

Ferret brain, rabies infected - THUNDER Imager 3D Cell Culture

Influenza in lung epithelial cells (porcine) - THUNDER Imager 3D Cell Culture

Lung Organoid - THUNDER Imager 3D Cell Culture

Pseudoislets (pancreatic beta cells) - THUNDER Imager 3D Cell Culture

Locust ganglion - THUNDER Imager 3D Tissue

HeLa cell spheroid - THUNDER Imager 3D Live Cell & 3D Cell Culture

Zebrafish - THUNDER Imager 3D Live Cell & 3D Cell Culture

Mouse kidney section - THUNDER Imager Tissue 3D

Cultured Cortical Neurons - THUNDER Imager 3D Cell Culture

Cultured VERO cells - THUNDER Imager 3D Cell Culture

YFP mouse brain slice - THUNDER Imager Tissue

Drosophila third instar larval - THUNDER Imager Tissue

Cyclops - THUNDER Imager Tissue

Zebrafish – THUNDER Imager Model Organism

Mouse - THUNDER Imager Model Organism

Organoid - THUNDER Imager Model Organism

Drosophila brain - THUNDER Imager 3D Live Cell

Honeybee leg - THUNDER Imager Model Organism

Paramecium - THUNDER Imager

Mouse kidney section - THUNDER Imager 3D Tissue

Mouse kidney section - THUNDER Imager 3D Tissue

Smooth Muscle - THUNDER Imager Model Organism

Primary culture, rat - THUNDER Imager 3D Cell Culture

Mouse kidney section - THUNDER Imager 3D Tissue

Zebrafish heart - THUNDER Imager Tissue

Zebrafish embryo – THUNDER Imager Model Organism

C2C12 cells - THUNDER Imager 3D Live Cell & 3D Cell Culture

Virally labeled neuron - THUNDER Imager Model Organism

Transgenic Drosophila photoreceptors of the eye - THUNDER Imager 3D Cell Culture

Cochlea cell - THUNDER Imager 3D Tissue

Neuromuscular junctions - THUNDER Imager 3D Tissue

Sea anemone polyp (Exaiptasia pallida) - THUNDER Imager Model Organism

Mouse lung - THUNDER Imager Tissue

Hippocampus showing an amyloid plaque - THUNDER Imager 3D Cell Culture

Neural crest (NC) embryonic cell population - THUNDER Imager 3D Cell Culture

Adult mouse ovary - THUNDER Imager 3D Cell Culture

C. elegans - THUNDER Imager Model Organism

Kidney Section - THUNDER Imager 3D Tissue

Retina Section - THUNDER Imager 3D Tissue

Heart Section - THUNDER Imager 3D Tissue

Liver Section - THUNDER Imager 3D Tissue

Brain Section - THUNDER Imager 3D Tissue

Mouse Retina - THUNDER Imager 3D Cell Culture

Mouse Aorta - THUNDER Imager 3D Tissue

Fiber - THUNDER Imager 3D Cell Culture

Mouse Lens Section - THUNDER Imager 3D Cell Culture

Human hepatic progenitor cell (HPC) - THUNDER Imager 3D Tissue

Mouse lung - THUNDER Imager 3D Cell Culture

Drosophila Follicle - THUNDER Imager 3D Cell Culture

Brine Shrimp leg - THUNDER Imager 3D Cell Culture

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