Contact & Support
Contact & Support
Contact
Gallery

Image Gallery: THUNDER Imager

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve this using Computational Clearing our new opto-digital technology. The result is high-speed, high-quality imaging of a large diversity of three-dimensional samples, including model organisms, tissue sections, and 3D cell cultures. Take a look at these images to see how THUNDER Imagers are already helping researchers to reveal the finest structural details even deep within a sample.   Once you have seen the haze-free image quality achieved with THUNDER Imagers, find out more on our product page. Learn how THUNDER Imagers combine Computational Clearing with the speed, fluorescence-signal sensitivity, and ease-of-use of widefield microscopes so you can decode 3D biology in real time*.

*in accordance with ISO/IEC 2382:2015

Authors

Topics & Tags

Locust ganglion - THUNDER Imager Tissue 3D

Locust ganglion - THUNDER Imager Tissue 3D
Image of a locust ganglion showing a maximum projection. Sample thickness is 110µm, data volume is 376 MB, and acquisition time using Computational Clearing is 3 seconds. Image taken by Korinna Schoch, Leica Microsystems, Germany.

HeLa cell spheroid - THUNDER Imager 3D Live Cell & 3D Cell Culture

HeLa cell spheroid - THUNDER Imager 3D Live Cell & 3D Cell Culture
HeLa cell spheroid stained with Alexa Fluor 568 Phalloidin (Actin) and YOYO 1 iodide (Nucleus). Image taken by Dr. Jan Schumacher, Leica Microsystems, Germany.

Zebrafish - THUNDER Imager 3D Live Cell & 3D Cell Culture

Zebrafish - THUNDER Imager 3D Live Cell & 3D Cell Culture
Zebrafish larvae (72 hours post fertilization). The blood vessels are indicated with green (fluorescence). Sample courtesy of Dr. Almary Guerra and Dr. Didier Stainier, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.

Mouse kidney section - THUNDER Imager Tissue 3D

Mouse kidney section - THUNDER Imager Tissue 3D
Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA.

Cultured Cortical Neurons - THUNDER Imager 3D Cell Culture

Cultured Cortical Neurons - THUNDER Imager 3D Cell Culture
Cultured Cortical Neurons where green indicates beta-III-tubulin and blue nuclei. The image stack of 59 planes has a volume of 21 µm³. Sample courtesy of FAN GmbH, Magdeburg, Germany.

Cultured VERO cells - THUNDER Imager 3D Cell Culture

Cultured VERO cells - THUNDER Imager 3D Cell Culture
Cultured VERO cells stained with STAR488 Vimentin (green), STAR580 Tom20 (yellow), and DAPI (blue). Sample courtesy of Abberior GmbH, Göttingen, Germany.

YFP mouse brain slice - THUNDER Imager Tissue

YFP mouse brain slice - THUNDER Imager Tissue
YFP mouse brain slices stained with GFAP-A647. Imaged with a THUNDER Imager Tissue. Courtesy of Dr. Hong Xu, University of Pennsylvania, Philadelphia, USA.

Drosophila third instar larval - THUNDER Imager Tissue

Drosophila third instar larval - THUNDER Imager Tissue
Drosophila third instar larval fillet labeled with AlexaFluor™647 for post-synaptic sites, AlexaFluor™555 conjugated to phalloidin, and AlexaFluor™488 labeling a subset of motor neurons. Sample courtesy of Dr. Amicia Elliott, NIH/NIMH, Bethesda, MD, USA.

Cyclops - THUNDER Imager Tissue

Cyclops - THUNDER Imager Tissue
Cyclops sp. showing the nuclei (green), acetylated -tubulin (red), and serotonin (cyan). The image stack has a total volume of 332 µm x 332 µm x 84 µm with three colors and 305 z planes per color. Objective used was HC PL FLUOTAR 40x/1.30 OIL. Sample courtesy of Dipl. Biol. Thomas Frase, University of Rostock, Allgemeine & Spezielle Zoologie, Institut for Biosciences, Rostock, Germany.

Zebrafish – THUNDER Imager Model Organism

Video of zebrafish larva (1x Plan APO objective, zoom factor 11:1, 18 z-slice stack, circa 300 µm z-depth) where green (kdrl:GFP) shows angiogenisis and red (GATA1:dsRed) the red blood cells and platelets. Courtesy of Dr. Almary Guerra and Dr. Didier Stainier, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.

Cleared Mouse - THUNDER Imager Model Organism

Cleared Mouse - THUNDER Imager Model Organism
In this E12-14 mouse (wt sample), neurofilaments are stained in red to assess neuronal outgrowth. The mouse was cleared with the ScaleS reagent. Courtesy of Yves Lutz, Centre d’imagerie, IGBMC, France.

Organoid - THUNDER Imager Model Organism

Organoid - THUNDER Imager Model Organism
An organoid approximately 150 µm in diameter mounted onto a depression slide. Imaged with a stereo microscope using a high resolution objective (FluoCombi III). Image taken by Heinrich Bürgers, Leica Microsystems, Switzerland.

Drosophila brain - THUNDER Imager 3D Live Cell

Video of a drosophila brain. Courtesy of Rohan Chippalkatti, Institute of Cell Biology, Bern, Switzerland.

Honeybee leg - THUNDER Imager Model Organism

Honeybee leg - THUNDER Imager Model Organism
A honeybee’s leg. Image taken by Dr. André Quinkertz, Leica Microsystems, Germany.

Paramecium - THUNDER Imager

Paramecium - THUNDER Imager
The paramecium is a unicellular ciliate microorganism which is commonly found in stagnant water, such as ponds. Image taken by Adam Cliffe, Leica Microsystems, Singapore.

Mouse kidney section - THUNDER Imager Tissue 3D

Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA.

Mouse kidney section - THUNDER Imager Tissue 3D

Mouse kidney section - THUNDER Imager Tissue 3D
Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA.

Smooth Muscle - THUNDER Imager Model Organism

Smooth Muscle - THUNDER Imager Model Organism
Smooth muscle around blood vessels in a 300 µm section of mouse lung. 1x objective. Courtesy of Dr. Mario Boehm, University of Giessen, Germany

Primary culture, rat - THUNDER Imager 3D Cell Culture

Primary culture, rat - THUNDER Imager 3D Cell Culture
Primary cell culture from rat; Cy5 (magenta): Beta-III tubulin; Rhod (red): NG2 protein, GFP (green): Nestin protein; and DAPI (blue): nuclei. Image taken by Louise Bertrand, Leica Microsystems, USA.

Mouse kidney section - THUNDER Imager Tissue 3D

Mouse kidney section - THUNDER Imager Tissue 3D
Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA.

Zebrafish heart - THUNDER Imager Tissue

Zebrafish heart - THUNDER Imager Tissue
Zebrafish heart, DAPI (nuclei, blue), Tropomyosin (cardiomyocytes, red) and GFP (primordial cardiac layer, green). Courtesy of Anna Jazwinska, University of Fribourg, France.

Zebrafish embryo – THUNDER Imager Model Organism

Zebrafish embryo 6 dpf with stable mCherry-expressing of HPCs, GFP-expressing of VEC. Courtesy of Dr.Yu Xue, Minnan Normal University, China.

C2C12 cells - THUNDER Imager 3D Live Cell & 3D Cell Culture

C2C12 cells - THUNDER Imager 3D Live Cell & 3D Cell Culture
C2C12 cells were grown and migrated onto both sides of a transwell membrane and stained with lamin B (magenta) for nuclear structure, Hoechst (blue) for DNA, and γH2AX (yellow) for DNA damage. Cells were imaged using a THUNDER Live Cell Culture imaging system with a 63X/1.4 oil immersion objective. Images above represent extended depth of field projections of 17.47 m thick z-stacks. The image on the left image is the raw epifluorescence data; the image on the right is the THUNDER-processed image, after LVCC. Images courtesy of Dr. Lucas Smith, Department of Neurobiology, Physiology and Behavior, College of Biological Sciences, University of California at Davis, Davis CA.

Virally labeled neuron - THUNDER Imager Model Organism

Virally labeled neuron - THUNDER Imager Model Organism
Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imager with a 2x 0.15 NA objective at 3.4x zoom was used to produce this 425 µm Z-stack (26 positions), which is presented here as an Extended Depth of Field (EDoF) projection. Images courtesy of Dr. Fikri Birey from the Dr. Sergiu Pasca laboratory, CA, USA.

Transgenic Drosophila photoreceptors of the eye - THUNDER Imager 3D Cell Culture

Transgenic Drosophila photoreceptors of the eye with EGFP labeled rhabdomeres under the control of the rhodopsin 1 promoter (ninaE-EGFP). Image Courtesy Navid Nouri, Genentech Inc., South San Francisco, California, USA.

Cochlea cell - THUNDER Imager 3D Tissue

Cochlea cell - THUNDER Imager 3D Tissue
Post-hatch day 7 chicken cochlea cells. 80µm vibratome section, Myosin 7a - sensory hair cells, Sox2 - supporting cells Image courtesy Amanda Janesick, CA, USA.

Neuromuscular junctions - THUNDER Imager

Neuromuscular junctions - THUNDER Imager
Neuromuscular junctions visualized by immunostaining of neurofilament and β-Bungarotoxin Image courtesy Andrea Yung, CA, USA.

Sea anemone polyp (Exaiptasia pallida) - THUNDER Imager Model Organism

Sea anemone polyp (Exaiptasia pallida) - THUNDER Imager Model Organism
Sea anemone polyp (Exaiptasia pallida) in RFP and GFP fluorescence channels under a Leica THUNDER stereo-microscopy system. The anemone hosts symbiotic dinoflagellate algae in its gastrodermal cells which can be seen in the RFP channel due to their chlorophyll autofluorescence. The anemone itself displays green fluorescence around its mouth and as bands around some tentacles. Image courtesy Christian Renicke, CA, USA.

Mouse lung - THUNDER Imager Tissue

Mouse lung - THUNDER Imager Tissue
Mouse lung, stained with FITC, Cy3, Alexa 633, taken with a 20x objective and extended depth of field (EDOF). Image courtesy Ross Metzger, CA, USA.

Related Articles

Related Products

THUNDER Imager Model Organism

The THUNDER Imager Model Organism allows fast and easy 3D exploration of whole organisms for developmental or molecular biology research.

THUNDER Imager 3D Live Cell & 3D Cell Culture & 3D Assay

THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids.

THUNDER Imager Tissue

The THUNDER Imager Tissue allows real-time fluorescence imaging of 3D tissue sections typically used in neuroscience and histology research.