Image Gallery: THUNDER Imager

Combine computational clearing with speed, fluorescence-signal sensitivity, and ease-of-use of widefield microscopes to decode 3D biology in real time*

Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture. Image courtesy of Dr. Selina Keppler, Munich, Germany. Mouse-lymphnode_THUNDER.jpg

To help you answer important scientific questions, THUNDER Imagers eliminate the out-of-focus blur that clouds the view of thick samples when using camera-based fluorescence microscopes. They achieve this using Computational Clearing our new opto-digital technology. The result is high-speed, high-quality imaging of a large diversity of three-dimensional samples, including model organisms, tissue sections, and 3D cell cultures. Take a look at these images to see how THUNDER Imagers are already helping researchers to reveal the finest structural details even deep within a sample.

Once you have seen the haze-free image quality achieved with THUNDER Imagers, find out more on our product page. Learn how THUNDER Imagers combine Computational Clearing with the speed, fluorescence-signal sensitivity, and ease-of-use of widefield microscopes so you can decode 3D biology in real time* (*in accordance with ISO/IEC 2382:2015)

Mouse lymphnode - Getting the complete picture for inflammation research

Mouse lymphnode acquired with a THUNDER Imager 3D Cell Culture. Video courtesy of Dr. Selina Keppler, Munich, Germany.

A part of the work at the Keppler lab involves the use of pre-clinical models for inflammatory disease to understand the interplay between cells during inflammation. In order to understand the distribution of the cells within the organ, the lab researchers needed to change from imaging 2D cryo-sections of lymph nodes to a method that is capable of viewing the whole organ.

They developed a clearing protocol to prepare the lymph nodes for imaging. Initially, they performed the imaging experiments using a confocal microscope, but this way took up to 18 hours to image just half of a lymph node. They could not use traditional widefield systems, because the autofluorescence coming from the tissue makes it impossible to see any significant amount of the desired signal.

Only with a THUNDER Imager, we could consider using a widefield-based system to study our organs

Dr. Selina Keppler, Munich, Germany.

Image the cells of interest within the lymph node in only a few minutes

Using a THUNDER Imager, the Keppler lab researchers found that they could clearly image the cells of interest within the lymph node in only a few minutes. In the example shown, they were able to clearly study the distribution of follicular cells (yellow), and blood vessels (magenta). Then they went on to study differences in wild type vs. inflamed lymph nodes. Having this initial overview in just 15 minutes helped them develop a more efficient screening workflow where they could then select the areas they wanted to analyze in detail using confocal microscopy enabling more detail segmentation or single cell analysis.

The THUNDER Imager has made our research more efficient, in terms of time savings, and more complete, as we can now look at whole structures and are not limited at just looking at parts of the organ

Dr. Selina Keppler, Munich, Germany.

Arabidopsis thaliana root - THUNDER Imager

Arabidopsis thaliana root; Early endosome marker protein (fused to GFP); Maximum projected Z-stack over time
Image courtesy: Prof. Dr. Karin Schumacher, Centre for Organismal Studies Heidelberg (COS), Germany

Hela Cells - THUNDER Imager

Hela cells stained with the live cell dye SIR-Tubulin. The video shows a direct comparison of widefield and TIRF illumination plus how different THUNDER methods (here ICC and LVCC) improve even more the contrast impression in the images. TIRF was performed using a THUNDER Imager 3D Live Cell with Infinity TIRF by using the 638nm Laser line and 110nm penetration depth. Timelapse and recording was done over 5 minutes. A small Z-Stack was recorded to gain enough data for THUNDER LVCC (stacksize: 1µm, 5 planes). Courtesy of Dr. Oliver Schlicker, Leica Microsystems

Mouse brain cortex - THUNDER Imager

Mouse brain cortex - THUNDER Imager
Mouse brain cortex displaying 500 nm green fluorescent beads in perivascular space. Blood vessels are stained with WGA lectin conjugated with Alexa 594 fluorophore. Sample courtesy of PhD Mika Kaakinen and M.Sc. Abhishek Singh, Biocenter Oulu, University of Oulu, Finland. Sample imaged by Janne Ylärinne, PhD, Immuno Diagnostic Oy.

Mouse embryonic kidney - THUNDER Imager

Mouse embryonic kidney - THUNDER Imager
Mouse embryonic kidney at day 12.5. E-Cadherin (red), unspecific (green). Sample prepared by MSc Kristen Kurtzeborn and used courtesy of Prof. Satu Kuure, Kidney Development group, University of Helsinki, Finland. Sample imaged by Janne Ylärinne, PhD, Immuno Diagnostic Oy.

Murine esophageal organoids - THUNDER Imager

Murine esophageal organoids - THUNDER Imager
Murine esophageal organoids. Integrin alpha6 (AlexaFluor 488, green), Sox2 (AlexaFluor 568, red), Nucleus (Dapi, blue).
Sample courtesy of Dr. Fabio Tadeu Arroso Martins, Tampere University, Finland. Sample imaged by Janne Ylärinne, PhD, Immuno Diagnostic Oy.

Mouse whole mount retina - THUNDER Imager

Mouse whole mount retina: Iba1+ microglial cells (Alexa Fluor® 488), 3D view (colour coded) showing two layers of glial cells (40x/0.95, LVCC). Image courtesy of Experimental Ophthalmology group, University of Murcia, Spain.

1 mm mouse brain section - THUNDER Imager

1 mm mouse brain section. CUBIC cleared. Stainded blood vessels and nuclei.
Video courtesy of Prof. Xiaowei Li, Shanghai Jiao Tong University, China

C. elegans. Transgenic GFP - THUNDER Imager

C. elegans. Transgenic GFP.
Video courtesy of Prof. Long Ma, School of Life Sciences, Central South University, China

Mouse cortical neurons - THUNDER Imager

Mouse cortical neurons. Transgenic GFP (green).
Image courtesy of Prof. Hui Guo, School of Life Sciences, Central South University, China

Adult Drosophila muscle - THUNDER Imager

Adult Drosophila muscle. Actin (Phalloidin-Cy3, red), Mitochondria (mito-GFP, green).
Video courtesy of Prof. Jieqiong Tan, School of Life Sciences, Central South University, China.

Drosophila Embryo - THUNDER Imager

Drosophila embryo expressing GCaMP in the CNS to visualize neural activity during development.
Video courtesy of Arnaldo Carreira-Rosario, USA

Mouse heart - THUNDER Imager 3D Cell Culture

Perinatal day 5 mouse heart Col1a1-GFP transgene.
Courtesy of Prof. Michelle Tallquist, University of Hawaii, Honolulu, USA

Drosophila Follicles - THUNDER Imager 3D Cell Culture

The follicles were imaged on a THUNDER 3D Cell Culture imaging system with a 63X/1.4 oil immersion objective. Images above represent 3D maximum projections of a 27.5 µm thick z-stack.
Image courtesy of Mark Khoury and David Bilder, University of California, Berkeley, USA.

Cortical neurons - THUNDER Imager

Cortical neurons - THUNDER Imager
Cortical neurons cultured in microfluidic chamber for 10 days. Fixation with PFA 4%. Immunofluorescence Tubulin alpha (green), Tau (red), DAPI (blue).
Image courtesy of Wei Wang, USA

Microglia in hippocampal region - THUNDER Imager

Immunostaining of wild type mouse showing hippocampus CAB region. Staining: Microglia cells (green), Neurons (magenta), Nuclei (cyan).
Image courtesy of Jenny Luna-Choconta, Medizinische Universität Innsbruck, Innsbruck, Austria.

Organoid Cluster - THUNDER Imager

Organoid Cluster, stained with DAPI - Nucleus, GFP - Plasma Membrane.
Image courtesy of Dana Krauß, Cancer Research Institue, Medical University of Vienna, Vienna, Austria.

Cranial nerves development - THUNDER Imager 3D Cell Culture

The images were collected with a 20x, multi-immersion objective with a numerical aperture of 0.75 and a working distance close to 700 µm. The image consists of 32 stitched tiles with an imaging depth of 672 µm (337 steps).
Image courtesy of Anastas Popratiloff, George Washington University, Washington DC, USA

Pancreatic islet - THUNDER Imager 3D Cell Culture

EDoF reconstruction of an isolated human islet to experimentally examine the expression of IL-17, a proinflammatory cytokine, in individual human islet cells. The images have the following markers: Insulin (AF488; green), Glucagon (AF555; red) and IL-17 (AF647; magenta) and Hoechst (nuclei; blue).
Image courtesy of the Matthias Von Herrath Lab at the La Jolla Institute of Immunology, La Jolla, CA., USA

Mouse Brain - THUNDER Imager

Red - AMPK, Green - Iba1.
Image courtesy of Maheedhar Kodali, Ashok K. Shetty, Texas A&M University, USA

Pollen Flower - THUNDER Imager

Taken with a 20x/0.8 objective, area of 6mm² with a depth of 100μm.
15 stitched tiles with 4 colors (DAPI/GFP/TRITC/Cy5) - a total of 13020 images.
Video courtesy of James Marr, Leica Microsystems, USA

C. elegans Gonades - THUNDER Imager

Adult hermaphrodit, Staining: blue - DAPI (Nucleus), green - SP56 (sperms), red - RME-2 (oocyte), mangenta - PGL-1 (RNA + protein granules).
Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany

Mouse aorta - THUNDER Imager 3D Live Cell

RAW Time Lapse - One week cultured explants of abdominal aorta imaged for 48 hours on gelatin coated #1.5 coverglass chamber slides. The mouse was genetically encoded with a smooth muscle cell specific tdtomato. After a transcription event, the smooth muscle cells excise the tdtomato and begin to express eGFP.
Image courtesy of Laura S. Shankman, Ph.D, University of Virginia, Charlottesville, USA.

Mouse aorta - THUNDER Imager 3D Live Cell

THUNDER Time Lapse - One week cultured explants of abdominal aorta imaged for 48 hours on gelatin coated #1.5 coverglass chamber slides. The mouse was genetically encoded with a smooth muscle cell specific tdtomato. After a transcription event, the smooth muscle cells excise the tdtomato and begin to express eGFP.
Image courtesy of Laura S. Shankman, Ph.D, University of Virginia, Charlottesville, USA.

Mouse Dystrophin staining on muscle fibres - THUNDER Imager

Infected MDCK epithelial cells - THUNDER Imager

Infected MDCK epithelial cells - THUNDER Imager
MDCK epithelial cells infected with Salmonella typhimurium reveal extensive actin rearrangement (red) induced by the bacteria (green, GFAP protein in fibrillae).
Objective: HC PL APO 40x/0.95 DRY.
Image courtesy of Dr. Mark Jepson, Bristol University, Bristol, UK.

Ferret brain, rabies infected - THUNDER Imager 3D Cell Culture

DISCO, Ferret brain, rabies infected - THUNDER Imager 3D Cell Culture
Rabies-infected neurons – red, Nuclei – blue. Chemically cleared with the DISCO method. Objective: HC PL FLUOTAR L 20x/0.40 DRY, 410 µm z-stack.
Image courtesy of Dr. Stefan Finke, Friedrich-Loeffler- Institute, Riems, Germany.

Influenza in lung epithelial cells (porcine) - THUNDER Imager 3D Cell Culture

Influenza virus – red, cilia – green, Nuclei – blue.
Objective: HC PL APO 40x/0.95 DRY, 35 µm z-stack, Max projection.
Image courtesy of Dr. Stefan Finke, Friedrich-Loeffler-Institute, Riems, Germany

Lung Organoid - THUNDER Imager 3D Cell Culture

Mouse lung organoids derived from alveola stem and progenitor cells. 20x Air through 1mm plastic bottom. Sample courtesy Dr. Pumaree Kanrai, MPI for Heart and Lung Research, Bad Nauheim, Germany.

Pseudoislets (pancreatic beta cells) - THUNDER Imager 3D Cell Culture

MIN6 cells grown as pseudoislets (pancreatic beta cells). DAPI (blue), Insulin (Alexa488, green), membrane receptor (Alexa594, red), phalloidin (Alexa647, white).
Sample courtesy Dr. Rémy Bonnavion, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.

Locust ganglion - THUNDER Imager 3D Tissue

Locust ganglion - THUNDER Imager 3D Tissue
Image of a locust ganglion showing a maximum projection. Sample thickness is 110µm, data volume is 376 MB, and acquisition time using Computational Clearing is 3 seconds. Image taken by Korinna Schoch, Leica Microsystems, Germany.

HeLa cell spheroid - THUNDER Imager 3D Live Cell & 3D Cell Culture

HeLa cell spheroid - THUNDER Imager 3D Live Cell & 3D Cell Culture
HeLa cell spheroid stained with Alexa Fluor 568 Phalloidin (Actin) and YOYO 1 iodide (Nucleus).
Image taken by Dr. Jan Schumacher, Leica Microsystems, Germany.

Zebrafish - THUNDER Imager 3D Live Cell & 3D Cell Culture

Zebrafish - THUNDER Imager 3D Live Cell & 3D Cell Culture
Zebrafish larvae (72 hours post fertilization). The blood vessels are indicated with green (fluorescence). Sample courtesy of Dr. Almary Guerra and Dr. Didier Stainier, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.

Mouse kidney section - THUNDER Imager Tissue 3D

Mouse kidney section - THUNDER Imager Tissue 3D
Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA.

Cultured Cortical Neurons - THUNDER Imager 3D Cell Culture

Cultured Cortical Neurons - THUNDER Imager 3D Cell Culture
Cultured Cortical Neurons where green indicates beta-III-tubulin and blue nuclei. The image stack of 59 planes has a volume of 21 µm³. Sample courtesy of FAN GmbH, Magdeburg, Germany.

Cultured VERO cells - THUNDER Imager 3D Cell Culture

Cultured VERO cells - THUNDER Imager 3D Cell Culture
Cultured VERO cells stained with STAR488 Vimentin (green), STAR580 Tom20 (yellow), and DAPI (blue). Sample courtesy of Abberior GmbH, Göttingen, Germany.

YFP mouse brain slice - THUNDER Imager Tissue

YFP mouse brain slice - THUNDER Imager Tissue
YFP mouse brain slices stained with GFAP-A647. Imaged with a THUNDER Imager Tissue. Courtesy of Dr. Hong Xu, University of Pennsylvania, Philadelphia, USA.

Drosophila third instar larval - THUNDER Imager Tissue

Drosophila third instar larval - THUNDER Imager Tissue
Drosophila third instar larval fillet labeled with AlexaFluor™647 for post-synaptic sites, AlexaFluor™555 conjugated to phalloidin, and AlexaFluor™488 labeling a subset of motor neurons. Sample courtesy of Dr. Amicia Elliott, NIH/NIMH, Bethesda, MD, USA.

Cyclops - THUNDER Imager Tissue

Cyclops - THUNDER Imager Tissue
Cyclops sp. showing the nuclei (green), acetylated -tubulin (red), and serotonin (cyan). The image stack has a total volume of 332 µm x 332 µm x 84 µm with three colors and 305 z planes per color. Objective used was HC PL FLUOTAR 40x/1.30 OIL. Sample courtesy of Dipl. Biol. Thomas Frase, University of Rostock, Allgemeine & Spezielle Zoologie, Institut for Biosciences, Rostock, Germany.

Zebrafish – THUNDER Imager Model Organism

Video of zebrafish larva (1x Plan APO objective, zoom factor 11:1, 18 z-slice stack, circa 300 µm z-depth) where green (kdrl:GFP) shows angiogenisis and red (GATA1:dsRed) the red blood cells and platelets. Courtesy of Dr. Almary Guerra and Prof. Dr. Didier Stainier, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany.

Mouse - THUNDER Imager Model Organism

Uncleared Mouse - THUNDER Imager Model Organism
In this E12-14 mouse (wt sample), neurofilaments are stained in red to assess neuronal outgrowth. The mouse was uncleared. Courtesy of Yves Lutz, Centre d’imagerie, IGBMC, France.

Organoid - THUNDER Imager Model Organism

Organoid - THUNDER Imager Model Organism
An organoid approximately 150 µm in diameter mounted onto a depression slide. Imaged with a THUNDER Imager Model Organism using a high resolution objective (FluoCombi III). Image taken by Heinrich Bürgers, Leica Microsystems, Switzerland.

Drosophila brain - THUNDER Imager 3D Live Cell

Video of a drosophila brain. Courtesy of Rohan Chippalkatti, Institute of Cell Biology, Bern, Switzerland.

Honeybee leg - THUNDER Imager Model Organism

Honeybee leg - THUNDER Imager Model Organism
A honeybee’s leg. Image taken by Dr. André Quinkertz, Leica Microsystems, Germany.

Paramecium - THUNDER Imager

Paramecium - THUNDER Imager
The paramecium is a unicellular ciliate microorganism which is commonly found in stagnant water, such as ponds. Image taken by Adam Cliffe, Leica Microsystems, Singapore.

Mouse kidney section - THUNDER Imager 3D Tissue

Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA.

Mouse kidney section - THUNDER Imager 3D Tissue

Mouse kidney section - THUNDER Imager Tissue 3D
Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA.

Smooth Muscle - THUNDER Imager Model Organism

Smooth Muscle - THUNDER Imager Model Organism
Smooth muscle around blood vessels in a 300 µm section of mouse lung. 1x objective.
Courtesy of Dr. Mario Boehm, University of Giessen, Germany

Primary culture, rat - THUNDER Imager 3D Cell Culture

Primary culture, rat - THUNDER Imager 3D Cell Culture
Primary cell culture from rat; Cy5 (magenta): Beta-III tubulin; Rhod (red): NG2 protein, GFP (green): Nestin protein; and DAPI (blue): nuclei. Image taken by Louise Bertrand, Leica Microsystems, USA.

Mouse kidney section - THUNDER Imager 3D Tissue

Mouse kidney section - THUNDER Imager Tissue 3D
Mouse kidney section with Alexa Fluor™ 488 WGA, Alexa Fluor™ 568 Phalloidin, and DAPI. Sample is a FluoCells™ prepared slide #3 from Thermo Fisher Scientific, Waltham, MA, USA.

Zebrafish heart - THUNDER Imager Tissue

Zebrafish heart - THUNDER Imager Tissue
Zebrafish heart, DAPI (nuclei, blue), Tropomyosin (cardiomyocytes, red) and GFP (primordial cardiac layer, green). Courtesy of Anna Jazwinska, University of Fribourg, Switzerland.

Zebrafish embryo – THUNDER Imager Model Organism

Zebrafish embryo 6 dpf with stable mCherry-expressing of HPCs, GFP-expressing of VEC.
Courtesy of Dr.Yu Xue, Minnan Normal University, China.

C2C12 cells - THUNDER Imager 3D Live Cell & 3D Cell Culture

C2C12 cells - THUNDER Imager 3D Live Cell & 3D Cell Culture
C2C12 cells were grown and migrated onto both sides of a transwell membrane and stained with lamin B (magenta) for nuclear structure, Hoechst (blue) for DNA, and γH2AX (yellow) for DNA damage. Cells were imaged using a THUNDER Imager 3D Live Cell with a 63X/1.4 oil immersion objective. Images above represent extended depth of field projections of 17.47 µm thick z-stacks. The image on the left image is the raw epifluorescence data; the image on the right is the THUNDER-processed image, after LVCC.
Images courtesy of Dr. Lucas Smith, Department of Neurobiology, Physiology and Behavior, College of Biological Sciences, University of California at Davis, Davis CA, USA.

Virally labeled neuron - THUNDER Imager Model Organism

Virally labeled neuron - THUNDER Imager Model Organism
Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Imager Model Organism with a 2x 0.15 NA objective at 3.4x zoom was used to produce this 425 µm Z-stack (26 positions), which is presented here as an Extended Depth of Field (EDoF) projection.
Images courtesy of Dr. Fikri Birey from the Dr. Sergiu Pasca laboratory, CA, USA.

Transgenic Drosophila photoreceptors of the eye - THUNDER Imager 3D Cell Culture

Transgenic Drosophila photoreceptors of the eye with EGFP labeled rhabdomeres under the control of the rhodopsin 1 promoter (ninaE-EGFP).
Courtesy of Navid Nouri, Genentech Inc., South San Francisco, California, USA.

Cochlea cell - THUNDER Imager 3D Tissue

Cochlea cell - THUNDER Imager 3D Tissue
Post-hatch day 7 chicken cochlea cells. 80µm vibratome section, Myosin 7a - sensory hair cells, Sox2 - supporting cells.
Image courtesy of Amanda Janesick, CA, USA.

Neuromuscular junctions - THUNDER Imager 3D Tissue

Neuromuscular junctions - THUNDER Imager 3D Tissue
Neuromuscular junctions visualized by immunostaining of neurofilament and β-Bungarotoxin. Image courtesy Andrea Yung, CA, USA.

Sea anemone polyp (Exaiptasia pallida) - THUNDER Imager Model Organism

Sea anemone polyp (Exaiptasia pallida) - THUNDER Imager Model Organism
Sea anemone polyp (Exaiptasia pallida) in RFP and GFP fluorescence channels under a THUNDER Imager Model Organism. The anemone hosts symbiotic dinoflagellate algae in its gastrodermal cells which can be seen in the RFP channel due to their chlorophyll autofluorescence. The anemone itself displays green fluorescence around its mouth and as bands around some tentacles.
Image courtesy of Christian Renicke, CA, USA.

Mouse lung - THUNDER Imager Tissue

Mouse lung - THUNDER Imager Tissue
Mouse lung, stained with FITC, Cy3, Alexa 633, taken with a 20x objective and extended depth of field (EDOF).
Image courtesy of Ross Metzger, CA, USA.

Hippocampus showing an amyloid plaque - THUNDER Imager 3D Cell Culture

Hippocampus showing an amyloid plaque - THUNDER Imager 3D Cell Culture
Longitudinal mouse brain section of the hippocampus showing an amyloid plaque
(green, stained with 6E10 antibody, marker of anti-beta amyloid) surrounded by microglia/microphages (red, stained with Anti-Abi1 antibody; blue, DAPI).
Left – Raw data with Extended Depth of Field projection;
Right – Large Volume Computationally Cleared Z-stack with Extended Depth of Field projection.
Images courtesy of Prof. Mehrdad Shamloo, CA, USA.

Neural crest (NC) embryonic cell population - THUNDER Imager 3D Cell Culture

Neural crest (NC) embryonic cell population - THUNDER Imager 3D Cell Culture
The image above was taken with a THUNDER Imager 3D Cell Culture using a 1.3 NA 40x plan apo oil objective. The upper image is the raw widefield data. The lower image the result after the Leica proprietary Small Volume Computational Clearing (SVCC) has been applied to remove out-of-focus blur and better reveal the underlying structure within the sample.
Image courtesy of Michael Piacentino, BronnerLab, California Institute of Technology, Pasadena, CA, USA.

Adult mouse ovary - THUNDER Imager 3D Cell Culture

An adult mouse ovary was fixed and stained with anti-NOBOX, then cleared with modified 3DISCO. The ovary was placed in a homemade well with a coverslip bottom and imaged on a THUNDER Imager 3D Cell Culture imaging system with a 10X/0.32 objective.
Video represents 3D projections of a merged 4-tile mosaic, 586µm thick z-stack as raw epifluorescence and the THUNDER-processed image, after LVCC.
Video courtesy of Drs. Diana Laird and Bikem Soygur, University of California, San Francisco, USA

C. elegans - THUNDER Imager Model Organism

C. elegans - THUNDER Imager Model Organism
This strain MAH215 (Chang et al., Elife, 2017) is a dual-fluorescent mCherry::GFP::LGG-1 protein to monitor autophagic flux in C. elegans by visualizing both autophagosomes (APs), as well as, autolysosomes (ALs).
The green punctae (GFP) are APs while the ALs quench GFP in the acidic environment and emit only the mCherry signal.
Image courtesy of Dr. Aditi U. Gurkar, University of Pittsburgh, PA, USA

Kidney Section - THUNDER Imager 3D Tissue

Kidney Section - THUNDER Imager 3D Tissue
Kidney, stained with DAPI, WGA-FITC, Phalloidin-568​. z=20µm. Image courtesy of Dr. Kerstin Troidl​, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany

Retina Section - THUNDER Imager 3D Tissue

Retina Section - THUNDER Imager 3D Tissue
Retina section, stained with PECAM-488, AF-555. z=30µm. Image courtesy of Dr. Kerstin Wilhelm​, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany

Heart Section - THUNDER Imager 3D Tissue

Heart Section - THUNDER Imager 3D Tissue
Heart Section - THUNDER Imager 3D Tissue. Heart section stained with DAPI, αSMA-FITC, Vimentin-Cy3. z=40µm. Image courtesy of Dr. André Schneider​, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany

Liver Section - THUNDER Imager 3D Tissue

Liver Section - THUNDER Imager 3D Tissue
Liver section stained with DAPI, SERVA, CD31-594. z=70µm.
Image courtesy of Dr. Remy Bonnavion, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany

Brain Section - THUNDER Imager 3D Tissue

Brain Section - THUNDER Imager 3D Tissue
Brain section stained with DAPI, αGFAP-AF647. z=100µm. Image courtesy of Dr. Hong Xu, Max Planck Institute for Heart and Lung Research, Bad Nauheim, Germany

Mouse Retina - THUNDER Imager 3D Cell Culture

Mouse Retina - THUNDER Imager 3D Cell Culture
Mouse retina was fixed and stained by following reagents: anti-CD31 antibody (green): Endothelia cells, IsoB4 (red): Blood vessels, and microglia anti-GFAP antibody (blue): Astrocytes
Sample courtesy by Jeremy Burton, PhD and Jiyeon Lee, PhD, Genentech Inc., South San Francisco, USA. Imaged by Olga Davydenko, PhD (Leica)

Mouse Aorta - THUNDER Imager 3D Tissue

Mouse Aorta - THUNDER Imager 3D Tissue
Red line - elastic lamina, green and red - vascular cells, blue - DAPI, Image courtesy of Ying Wang, CA, USA
Red line - elastic lamina, green and red - vascular cells, blue - DAPI, displayed with the LAS X 3D Viewer. Image courtesy of Ying Wang, CA, USA

Fiber - THUNDER Imager 3D Cell Culture

Fiber - THUNDER Imager 3D Cell Culture
Fiber was labeled with fluorescein. Goal is to study the dynamics and pores. Z-stack of 55 images (130µm). Image courtesy of Mollie Smoak, Department of Bioengineering, Rice University, Houston, TX, USA

Mouse Lens Section - THUNDER Imager 3D Cell Culture

Mouse Lens Section - THUNDER Imager 3D Cell Culture
This slide is a transversal Mouse adult lens section showing the fiber lens cells organization (green, stained with anti-β-catenin antibody (BDB610153), a membrane protein) and Afadin (red, stained with anti-Afadin (PA1-25076), an actin filament-binding protein, blue, Hoechst).
Image courtesy of Dr. Nathalie Houssin, Plagemen Lab, Ohio State University, USA

Human hepatic progenitor cell (HPC) - THUNDER Imager 3D Tissue

Expansion and differentiation of human hepatic progenitor cell (HPC) in hepatocysts in organoids. The z-stack covers a total thickness of 85µm with 123 z-steps using a HC PL Fluotar 40x/0.60 objective. DAPI (white): Nuclei. Alexa488™(green): epithelial cell adhesion molecule (EpCAM). PerCP-Cy5.5 (red): signal transducer CD24. PE (blue): prominin-1 (CD133).
Sample Courtesy of Dr. Fatima Abukunna, University of Nottingham, E131 Andy Bennett Lab, Nottingham (UK).

Mouse lung - THUNDER Imager 3D Cell Culture

Mouse lung tissue stained for a study of type I alveolar epithelial cell (AT1) biology. Yellow indicates AT1 lineage trace (GFP), magenta the receptor for advance glycation end products (RAGE), cyan aquaporin 5 (AQP5), and gray Nuclei (DAPI). Image courtesy of Yana Kazadaeva, CA, USA.

Drosophila Follicle - THUNDER Imager 3D Cell Culture

The Drosophila follicles were imaged on a THUNDER Imager 3D Cell Culture with a 63X/1.4 oil immersion objective. Video above represent 3D maximum projections of a 27.5 µm thick z-stack. The video shows the raw epifluorescence data and the THUNDER-processed image, after LVCC.
Image courtesy of Mark Khoury and David Bilder, University of California, Berkeley, USA

Brine Shrimp leg - THUNDER Imager 3D Cell Culture

Brine Shrimp leg - THUNDER Imager 3D Cell Culture
Autofluorescence acquired with Rhod, GFP and DAPI filter cube.
Image acquisition performed with DMi8, HC PL APO 63x/1.40 Oil Objective and DFC9000 GT camera.
Image size 2048 x 2048 pixels, 274 z-sections.
Image taken by Louise Bertrand, Leica Microsystems, USA.

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