How to Successfully Implement Coral Life

Koenig, Julia; Kirmse, Robert

October 04, 2021

How to Successfully Implement Coral Life

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Many electron microscope (EM) workflows start with sample fixation followed by sample preparation and EM imaging. However, samples displaying interesting behavior tend to be rare and finding the “right cell” can be time-consuming and tedious. The live-cell  CLEM workflow allows you to capture dynamic information related to a relevant biological process as it happens and put these observations into their ultrastructural context. The Coral Life workflow streamlines this process to optimize your performance and increase your productivity. In this webinar, we will demonstrate the Coral Life workflow using an example which shows how reproducible results can be obtained without compromising sample quality.

Please note that the Leica Nano Workflow was renamed to Coral Life. The previous name Leica Nano Workflow is still used within the recorded webinar.

Speakers

Dr. Julia Koenig

Julia Koenig studied Biology at Dresden University of Technology. After finishing her PhD in 2015, she joined the Electron Microscopy Facility of Lucy Collinson at the Francis Crick Institute in London, working on a wide variety of projects as well as methods and workflow development. In January 2018, she joined Leica Microsystems as a product manager for EM sample preparation for cryo workflows.

Dr. Robert Kirmse

Robert Kirmse studied biology at the University of Heidelberg. After finishing his PhD at the German Cancer Research Center (DKFZ) in 2007 and staying at the DKFZ as a postdoctoral fellow he joined the group of Andreas Hoenger at the University of Colorado at Boulder in 2009. He then changed into industry in 2012 as a product manager and application expert for correlative microscopy at ZEISS. In 2019 he joined Leica to head the product management team for sample preparation. Robert has a broad experience in light and electron microscopy methods including correlative and cryo workflows.

In this webinar you will learn

How to maintain physiological conditions from cell culture until cryo fixation
How to optimize your live-cell imaging for live-cell CLEM applications
Easy transfer from the THUNDER Imager Nano to the EM ICE high pressure freezer
Retrieve your cell of interest in the resin block

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