Insights into Vesicle Trafficking

STELLARIS provides integral access to complementary layers of information for dynamic, structural, and mechanistic insights into vesicle trafficking.

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About the webinar

Imaging live cells is key to reveal their inner workings, dynamics, and function. Current technological advances in confocal imaging can benefit from novel fluorescence labeling strategies and biosensors.

With these advances, it is possible to probe biologically relevant processes faster and easier than before. Still, understanding the underlying mechanisms of any given process requires the observation of a specimen from different angles. Intensity-based imaging provides a reliable view of the spatial organization and distribution of intracellular species and organelles, whereas lifetime-based approaches can elicit information from the micro-environment and other functional related signals.

Moreover, fluorescence nanoscopies have evolved into the go-to techniques to examine structural details beyond the physical diffraction limits, with molecular specificity, and within the crowded cellular milieu.

In this talk we will show how STELLARIS provides an integral access to these different layers of information from dynamic, structural, and mechanistic insights into vesicle trafficking.

The endocytic vesicles shuttle materials between different cellular compartments, with the extracellular space, and the endocytic pathway is vital to almost all aspects of cell life and disease. This pathway is of particular interest in host-pathogen studies as many viruses and bacteria can exploit it as a cell entry point.

What to expect in the webinar

Key learnings

  • Discover how the Power HyD detector family contributes to the sensitivity of STELLARIS and how our lifetime-based technology TauSense enables the study micro-environmental changes (e.g. pH).
  • Understand how TauSTED works and how a fast lifetime-based readout is essential in its implementation.
  • Learn how the TauSTED approach delivers cutting-edge resolution and image quality at low light dose, key to studying nanoscale dynamics of cellular processes.

The speaker

Julia Roberti

PhD, Leica Microsystems

Julia studied Chemistry at the University of Buenos Aires, where she worked on Photochemistry and Analytical Chemistry. She focused on the characterization of luminescence of lanthanide complexes, and on the development of boron quantification methods for boron neutron capture therapy (BNCT) of cancer. After graduating, she moved to Göttingen to carry out her doctoral research at the Max Planck Institute for Biological Chemistry. She developed in vitro and in situ fluorescence labeling strategies to elucidate the oligomerization and aggregation mechanisms of the Parkinson's disease-associated protein alpha-synuclein. In 2012, she joined EMBL as Humboldt postdoctoral fellow, and applied advanced confocal microscopy and nanoscopy imaging to study chromatin compaction at the interphase-to-mitosis transition. She joined Leica Microsystems in 2017 as Product Manager for advanced confocal imaging.

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