Imaging live cells is key to reveal their inner workings, dynamics, and function. Current technological advances in confocal imaging can benefit from novel fluorescence labeling strategies and biosensors.
With these advances, it is possible to probe biologically relevant processes faster and easier than before. Still, understanding the underlying mechanisms of any given process requires the observation of a specimen from different angles. Intensity-based imaging provides a reliable view of the spatial organization and distribution of intracellular species and organelles, whereas lifetime-based approaches can elicit information from the micro-environment and other functional related signals.
Moreover, fluorescence nanoscopies have evolved into the go-to techniques to examine structural details beyond the physical diffraction limits, with molecular specificity, and within the crowded cellular milieu.