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Laser Microdissection Protocols for Tissue and Cell Isolation - Download free eBook

Bio-protocol collection: Applications of LMD from complex tissues to single cells

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Laser Microdissection (LMD, also known as Laser Capture Microdissection or LCM) enables users to isolate specific single cells or entire areas of tissue , and even subcellular structures such as chromosomes . Purified tissues and cells are used for downstream RNA, DNA, and proteomic workflows. In this Bio-protocol Selections, we present a collection of open-access, detailed methods papers using LCM to purify and isolate tissues and cells from plants, mouse embryos, cancer cells, neurons, and more.

What to learn from the ebook

Protocols include:

  • Laser Capture Micro-dissection (LCM) of Neonatal Mouse Forebrain for RNA Isolation
  • Laser Capture Microdissection (LCM) of Human Skin Sample for Spatial Proteomics Research
  • Isolation of Rice Embryo Single Cell Type using Laser Capture Microdissection (LCM)
  • Subcellular RNA-seq for the Analysis of Dendritic and Somatic Transcriptomes of Single Neurons

About Laser Capture Microdissection

Since it was developed in 1976, LMD technology has been an important tool, enabling researchers to precisely extract specific cell populations from heterogenous tissues. This precision became increasingly crucial with the development of molecular analysis techniques such as PCR, facilitating in-depth genomic, transcriptomic, and proteomic studies at the cellular level. 

About Bio-protocol

Established in 2011 by a group of postdoctoral researchers at Stanford University, Bio-protocol (www.bio-protocol.org) is an online peer-reviewed protocol journal. Its mission is to make life science research more efficient and reproducible by curating and hosting high quality, free access protocols. Bio-protocol journal has grown to approximately 5,000 protocols contributed by over 25,000 scientists worldwide. Bio-protocol editorial and reviewing boards consist mostly of postdocs and junior faculty members. These early-career life-science researchers, who actively and routinely design, conduct and supervise lab experiments, are well positioned to assess the quality and clarity of protocols.

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