Imaging-based approaches to identify skin immune cells are still challenging because of:
- the high autofluorescence from the tissue;
- the potential spectral spillover when using more than four fluorochromes simultaneously; and
- the entanglement of all cells within thick and polarized structural appendages.
There is a strong need to democratize new imaging systems using inexpensive instrumentation and consumables, compatible with standard tissue processing/analysis techniques and user-friendly enough to be used in routine clinical practice.
In this webinar, we introduce a simple method to acquire up to 10 colors using a conventional Leica SP8 microscope coupled to MANTIS (Multiplex ANnotated Tissue Imaging System). MANTIS is a flexible analytical system specifically designed for spatially resolved immune phenotyping of the skin in thick experimental samples or large clinical cohorts.