In patch clamp experiments a tight seal is formed between a glass pipette and the cell membrane. This arrangement allows the recording of small currents and voltages involved in the activity of neurons and other cells (Figure 1). A large observation field is necessary in order to find the regions of interest within a specimen, for example in the brain of a living mouse, living brain slices, embryos, etc. In addition, it is important to be able to visualise detailed structures so that pipettes and electrodes in the specimen can be placed accurately.
The Leica DM6000 CFS combines the new high NA/low magnification objective lens HC X