Image of C2C12 cells: The cells are stained with lamin B (magenta) which indicates nuclear structure, Hoechst (blue) indicating DNA, and γH2AX (yellow) indicating damage to DNA. Cells were imaged using a THUNDER Imager 3D Live Cell with a 63X/1.4 oil immersion objective.Image of C2C12 cells: The cells are stained with lamin B (magenta) which indicates nuclear structure, Hoechst (blue) indicating DNA, and γH2AX (yellow) indicating damage to DNA. Cells were imaged using a THUNDER Imager 3D Live Cell with a 63X/1.4 oil immersion objective.

Cell Biology Research

When your research is centered on understanding the cellular basis of human health and disease, it is critical to investigate the cells of interest in spatiotemporal and molecular detail. Consequently, microscopy is an ever-important tool in cell biology, allowing you to study your specimens in detail within their structural environment, as well as, analyze cellular organelles and macromolecules. Cell biology imaging is done with a range of light and electron microscopes. Imaging solutions offered by Leica Microsystems are designed to maximize your cell biology research.

Need Assistance?

Contact a local specialist for advice on the right cell biology system for your needs.

Challenges with cellular imaging

The challenges faced when using microscopy for cell biology research vary, as the imaging of inter- and intra-cellular events requires a range of samples that vary in size and complexity. Imaging of these events needs to be performed from the nanometer to the millimeter scale.

Additionally, the study of cells under the microscope is further influenced by whether they are live or fixed samples, as these cases pose different imaging challenges. One challenge is how to capture fast dynamic events with the right resolution. Another one, for any cellular structures or events that do not produce a natural contrast during imaging, is choosing the right fluorescent proteins, antibodies, or nucleic acid probes to use as tags for specific proteins, DNAs, and RNAs in the cell to be viewed with fluorescence microscopy.

Finding the right solution for cellular imaging

Choosing the right microscopy method is critical if you want to maximize your research output and obtain the highest quality data. Leica Microsystems offers various solutions to improve your cell biology research. These range from digital imagers that enhance daily cell culture tasks to high-end imaging solutions that enable the study of single molecules in detail, seamlessly move from fast widefield imaging to high resolution confocal imaging with just a simple click or maximize the amount of results you get from your sample.

THUNDER Imager

THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids. THUNDER Imager 3D Live Cell & 3D Cell Culture meets your requirements to study cells close to their physiological state by optimizing experimental conditions, e.g., the lowest light intensity and shortest exposure times possible.

Infinity TIRF

TIRF is ideal for imaging dynamic processes and the method of choice for visualizing single molecules with super-resolution.

STELLARIS

Experience the power to see more within your cells, with enhanced image quality, the possibility to image more markers within one sample at the same time, and the capacity to make detailed observations without influencing or damaging your sample with STELLARIS confocal microscope platform.

Cell biology related products

Filter by Area of Application
Mica - The world’s first Microhub

Mica

Mica. Everything you need to enable discoveries, unified in one easy-to-use system. Simultaneous 4-color widefield, confocal resolution, AI supported analysis.

Aivia

Access the future of AI microscopy. Take your image analysis further than ever before. Start a free trial.

Image of C2C12 cells: The cells are stained with lamin B (magenta) which indicates nuclear structure, Hoechst (blue) indicating DNA, and γH2AX (yellow) indicating damage to DNA. Cells were imaged using a THUNDER Imager 3D Live Cell with a 63X/1.4 oil immersion objective.
Featured image

Image of C2C12 cells

The cells are stained with lamin B (magenta) which indicates nuclear structure, Hoechst (blue) indicating DNA, and γH2AX (yellow) indicating damage to DNA. Cells were imaged using a THUNDER Imager 3D Live Cell with a 63X/1.4 oil immersion objective.

Images courtesy of Dr. Lucas Smith, Department of Neurobiology, Physiology and Behavior, College of Biological Sciences, University of California at Davis.

THUNDER Imager 3D Live Cell

Follow us on Instagram

Related Articles

Read our latest articles about Cell Biology Research

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments.

More Articles

Dual color volume rendering of Drp1 oligomers (green) and mito OM (red) in a live U2OS cell

Multicolor 4D Super Resolution Light Sheet Microscopy

The AI Microscopy Symposium offers a unique forum for discussing the latest AI-based technologies and tools in the field of microscopy and biomedical imaging. In this scientific presentation, Yuxuan…
Read article
Raw widefield (left) and THUNDER (right) image of Ewing Sarcoma cells (SK-ES-1)

Visualizing the Mitotic Spindle in Cancer Cells

This article demonstrates how this research is aided by visualizing more details of mitotic spindles in Ewing Sarcoma cells using the THUNDER Imager Tissue and Large Volume Computational Clearing…
Read article
4D morphological information of Arabidopsis thaliana cotyledon pavement cells mapped using both direct water immersion observations and computational image analyses, including segmentation, surface modeling, virtual reality and morphometry.

Mapping 4D cell models with VR

Plant researchers Takumi Higaki and Hidenobu Mizuno have developed a framework to map leaf pavement cells in 4D using AI software with VR equipment.
Read article
The EM ICE Nano loading area

How to Keep Your Samples Under Physiological Conditions

Leica Microsystems’ Nano workflow combines dynamic data with the best possible sample fixation (EM ICE 70) by high pressure freezing. However, good sample preservation won’t help if your cells are…
Read article

Putting Dynamic Live Cell Data into the Ultrastructural Context

With the Leica Nano Workflow, searching for a needle in the haystack is a thing of the past. Take advantage of correlative light and electron microscopy to identify directly the right cell at the…
Read article

A Quality Metric for the Systematic Evaluation of Clearing Protocols

3D multicellular spheroids are of interest for studying tumor behavior and evaluating the response of pharmacologically active agents, because they mimic the in vivo tumor environment better than…
Read article

Advancing Cell Biology with Cryo-Correlative Microscopy

Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…
Read article

Coronavirus Replication in Host Cells

In this study, a molecular pore complex, which spans both membranes of SARS-CoV-2/COVID-19 virus-induced cytosolic vesicles and likely plays a role in replication, was visualized.
Read article

Upright Fluorescence Microscopy for Virus Replication Studies

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2019 and spread over the world quickly. Due to its dramatic impact, researchers are interested in the nature of the…
Read article

Infection of the novel coronavirus SARS-CoV-2 can be blocked by a human monoclonal antibody

Vaccines and targeted therapeutics for treatment of the respiratory disease COVID-19, caused by the coronavirus SARS-CoV-2, are currently lacking. Based on the results of this study, it was reported…
Read article
Two-color SRS image of human pancreatic organoids showing proteins (red) and lipids (green).

Vibrational Spectroscopic Imaging in Cells, Tissues and Model Organisms

Here we present a webinar on the emerging applications of Stimulated Raman Scattering Microscopy, covering the following topics: Quantitative spectroscopic imaging of label-free or chemically tagged…
Read article

New Light Shed on the Nanodomain Organization of the Endoplasmic Reticulum (ER)

The endoplasmic reticulum (ER) is a continuous membrane organelle in charge of protein synthesis, lipid synthesis and detoxification. The ER structure is described in terms of smooth peripheral…
Read article

Imaging Intracellular Temperature using Fluorescence Lifetime Imaging Microscopy (FLIM)

This video shows the presentation of Dr. Kohki Okabe given at the 9th Congress of the Federation of Asian and Oceanian Physiological Society (FAOPS) held in Kobe, Japan on 30 March 2019. Dr. Okabe…
Read article

Development of fluorescence lifetime imaging microscopy (FLIM) and its relevance for functional imaging

Prof. Ammasi Periasamy, Director, Keck Center for Cellular Imaging, University of Virginia, was interviewed by Dr. Giulia Ossato, Product Manager functional imaging, during Leica Microsystems Meets…
Read article

How can PAULA help streamline the workflow for my wound healing assay?

The wound healing assay, often used as a synonym for scratch assay or migration assay, is an important tool for the evaluation and measurement of cell migration, whether single cells or cell…
Read article
MDCK cells, Phase Contrast, red outlines are marking the cell borders. Courtesy of Prof. Ralf Jacob, University of Marburg, Germany

Confluency Check with PAULA Cell Imager

Many cell-based experiments require cells in a certain state. This can include their morphology,  fluorescent protein expression, and the confluency of the cell layer. Researchers check their cells…
Read article

Kaggle Competition for Multi-label Classification of Cell Organelles in Proteome Scale Human Protein Atlas Data

The Cell Atlas, a part of the Human Protein Atlas, was created by the group of Prof. Emma Lundberg at the SciLifeLab, KTH Royal Institute of Technology, in Stockholm, Sweden. Currently, she is a…
Read article

SP8 DIVE Multiphoton Microscope Image Gallery

Today’s life science research focusses on complex biological processes, such as the causes of cancer and other human diseases. A deep look into tissues and living specimens is vital to understanding…
Read article

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?