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Fluorescence Microscopy

Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. Fluorescence is a form of luminescence. Fluorescence microscopy even allows users to determine the distribution of a single molecule species, its amount and its localization inside a cell. Colocalization and interaction studies can be performed, and ion concentrations as well as intra- and intercellular processes like endocytosis and exocytosis can be observed. With the help of super-resolution fluorescence microscopy, it is even possible to image sub-resolution structures.

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Our experts on solutions for fluorescence applications are happy to help you with their advice.

Fluorescence Light Microscopes

Fluorescence microscopes used in research applications are based on a set of optical filters:

  • an excitation filter
  • a dichroic beam splitter and
  • an emission filter

The filters are often plugged in together in a filter cube (compound microscopes) or in a flat holder (mainly stereo microscopes).

Whereas the excitation filter selects the wavelengths to excite a particular dye within the specimen, the emission filter serves as a kind of quality control by letting only the wavelengths of interest emitted by the fluorophore pass through. The dichroic mirror's purpose is to reflect light in the excitation band and transmit light in the emission band,  enabling the classic epifluorescence incident light illumination.

Fluorescence Stereo Microscopes

Leica Microsystems’ fluorescence stereo microscopes use the TripleBeam technology, a separate (third) beam path for fluorescence illumination of the sample without a dichroic mirror.

Therefore one excitation filter for the illumination beam path and two emission filters, one for each observation beam path, are needed.

Cameras for fluorescence microscopy

To acquire fluorescence images, it is necessary to use the appropriate CCD or sCMOS fluorescence camera. Leica Microsystems offers dedicated fluorescence cameras for all application needs. Each camera provides the perfect match of sensitivity and image quality to ensure superb results.

Software for fluorescence microscopy

Straightforward setup and control of experiments is becoming more and more important In fluorescence microscopy. The dedicated Leica Application Suite (LAS) X software platform facilitates the setup of even complex experiments. LAS X guides you from the setup to the analysis of the imaging data. From easy multi-channel experiments to complex 3D analysis, LAS X is the perfect software platform.

Fluorescence Microscopes & Imaging Products 31

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STELLARIS - Confocal Microscope Platform

STELLARIS

With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.

THUNDER Imager Model Organism

The THUNDER Imager Model Organism allows fast and easy 3D exploration of whole organisms for developmental or molecular biology research.

THUNDER Imager EM Cryo CLEM

The THUNDER Imager EM Cryo CLEM enables precise identification of cellular structures and smooth, secure transfer of coordinates, images, and samples through your correlative workflow.

K5

sCMOS Microscope Camera

THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay

THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids.

THUNDER Imager Tissue

The THUNDER Imager Tissue allows real-time fluorescence imaging of 3D tissue sections typically used in neuroscience and histology research.

Leica M205 FCA & Leica M205 FA

(Semi)-automated fluorescence stereo microscopes

High Speed High Resolution Monochrome Fluorescence sCMOS Camera Leica DFC9000

Leica DFC9000

4.2 MP cooled monochrome fluorescence scmos camera for advanced applications like high-speed live cell imaging, FRAP, and ratio measurement

2.8 MP Color Microscope Camera Leica DFC7000 T

DFC7000 T and DFC7000 GT

Cooled 2.8 megapixel digital microscope cameras

Leica DM4 B & DM6 B

Increase work efficiency with Leica DM4 B & DM6 B upright digital research microscopes.

Uniquely Ergonomic System Microscope Leica DM1000

Leica DM1000

Uniquely Ergonomic System Microscope

The Leica DM1000 LED combines the advantages of a fully ergonomic system microscope with innovative LED illumination, and unlimited possibilities in mobile use.

Leica DM1000 LED

Uniquely ergonomic system microscope with LED illumination

Ergonomic microscope system Leica DM2500 LED for demanding tasks in life science applications

Leica DM2500 & DM2500 LED

Ergonomic optical microscope systems for routine and research applications

The uniquely ergonomic system microscope Leica DM3000 boosts cytology and pathology workflows thanks to its intelligent automation.

Leica DM3000 & DM3000 LED

Uniquely Ergonomic System Microscopes With Intelligent Automation

Fluorescence Microscope Camera Leica DFC3000 G

Leica DFC3000 G

1.3 MP monochrome camera for basic fluorescence applications such as documentation of fixed, immunostained samples

Inverted Laboratory Microscope with LED Illumination

Leica DM IL LED

Inverted Laboratory Microscope with LED Illumination

Leica DM6 FS fixed stage fluorescence microscope

Leica DM6 FS

Fixed Stage Microscope for Electrophysiology and in vivo Imaging

Leica TL4000 BFDF

Transmitted Light Base For Brightfield and Darkfield Applications Absorbs Shock and Vibrations for High Quality Imaging

Simultaneous use of 1X stereo objective and 10X or 20X compound objective for high resolution imaging.

Leica FluoCombi III

Fluorescence stereomicroscope accessory

LED Multi Contrast Illumination LED3000 MCI

Leica LED3000 MCI

LED Multi Contrast Illumination for Routine Stereo Microscopes

The Leica LAS X Widefield System performs workflow-guided advanced image analysis and includes a full range of image enhancement tools.

LAS X Widefield Systems

Fluorescence Microscope System for Advanced Imaging and Analysis

About Fluorescence Microscopy

Fluorescence microscopy has become the most important physical phenomenon in modern biology and medicine.

After a slow start, the possibility of specific fluorescence staining boosted this application in the early 30's of the last century. Around 1950 a second stage was ignited with the invention of immunofluorescent staining.

Functional imaging, most prominently by Ca++ probes, delivered the method from the restraint to stick to morphological and structural imaging. The possibility to create living material with targeted expressible fluorescent proteins revolutionized fluorescence microscopy in an unexpected and extensive third stage.

Currently, new methods that use specific fluorescent protein-features, are popping up weekly. And last but not least: all modes of super-resolution imaging are based on fluorescence.

Useful Links

Communities and Web Sources

www.researchgate.net/
Social network for scientists

www.ibiology.org/
Teaching tools, video lectures on biology and microscopy

bitesizebio.com
Online magazine and community for molecular and cell biology researchers

www.somersault1824.com
Resource for high-end scientific illustrations, images and animations

Search Engines and Data Bases

www.cellimagelibrary.org
Public resource database of images, videos, and animations of cells

harvester.fzk.de/harvester
Bioinformatic meta search engine for genes and proteins

www.gopubmed.com
Search interface for pubmed

en.wikipedia.org/wiki/List_of_academic_databases_and_search_engines
List of academic databases and search engines

scholar.google.com
Beta of Google's search engine for scientific article abstracts

Journals

www.doaj.org/
Directory of open access journals

emboj.embopress.org/
The EMBO Journal

www.lifescied.org
CBE-Life Sciences Education – an ASCB online journal

www.sciencemag.org/
Science

www.nature.com/
Nature

www.cell.com/
Biweekly publication of exceptional research articles

jcs.biologists.org/
Journal of Cell Science

dev.biologists.org/
Development

jeb.biologists.org/
The Journal of Experimental Biology

dmm.biologists.org/
DMM Disease Models & Mechanisms

www.biotechniques.com/
International Journal of Life Science Methods

www.opticsinfobase.org/
Collection of Journals and Proceedings in Optics and Photonics

spie.org/x576.xml
SPIE - peer-reviewed journals on applied research in optics and photonics

onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648
Journal of Biophotonics

www.plosone.org/home.action
International, peer-reviewed, open-access, online publication

rspb.royalsocietypublishing.org/
Proceedings B - the Royal Society's biological research journal

www.microscopy-analysis.com/
International Journal for microscopists

Organizations / Institutes

www.microscopy.org/
Microscopy Society of America

www.eurmicsoc.org/
European Microscopy Society

www.rms.org.uk/
Royal Microscopical Society

www.doitpoms.ac.uk
Tutorials on microscopy for materials science; University of Cambridge, Department of Materials Science and Metallurgy

www.ascb.org/
ASCB American Society of Cell Biology

www.biologists.com/cob_activities.html
the company of biologists

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