Laser Microdissection LMD
Laser Microdissection, also known as LMD or LCM (Laser Capture Microdissection), is a contact- and contamination-free method for isolating specific single cells or entire areas of tissue from a wide variety of tissue samples. The thickness, texture and preparation technique of the original tissue are relatively unimportant. The dissectate is then available for further molecular biological methods such as PCR, real-time PCR, proteomics and other analytical techniques. Laser microdissection is now used in a large number of research fields, e.g. neurology, cancer research, plant analysis, forensics or climate research. The method is meanwhile also applied for manipulation of cell cultures or for microengraving of coverslips.
Our LMD Systems are perfect tools to optimize DNA-Workflows (Genomics), RNA-Workflows (Transcriptomics) and Proteomic-Workflows as they allow to precisely define and collect pure starting material for analysis under visual control.
Please contact us if you would like to have personal expert advice on our microscopy solutions for laser microdissection.
Leica Laser Capture Microdissection Products 2
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Leica LMD6 & LMD7
Laser Microdissection enables users to isolate specific single cells or entire areas of tissue.
Leica LMD Software
Laser Microdissection
Laser Microdissection Publication List
This list presents current publications in the major application fields for laser microdissection and is updated monthly.Read article
Medicinal Use of Qianhu Plant Roots
This study focused on tissue-specific metabolite profiling and comparison of various parts of bolting and unbolting Peucedanum praeruptorum Dunn (Qianhu in Chinese). The qualitative and quantitative…Read article
Analysis of Tissue Sections Assisted by Fluorescence Laser Microdissection
Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue…Read article
How to improve your RNA Analysis Workflow with Laser Microdissection
Brain research: Single neuron excision for RNA analysis - Improve your workflow with laser microdissection - Parkinson’s disease is a common progressive neurodegenerative disorder. It is connected…Read article
How to improve your Alzheimer Protein Analysis with Laser Microdissection
Brain Research: Collect pure starting material for proteomics - Improve your workflow with Laser Microdissection - Many brain diseases result from protein malfunction, misfolding and agglutination.…Read article
How to improve your DNA Mutation Analysis Workflow with Laser Microdissection
DNA mutations lead to abnormal proteins or missing functional proteins, which can cause cells to multiply uncontrollably and become cancerous. To find and understand the underlying mutation for a…Read article
Live Cell Isolation by Laser Microdissection
Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections,…Read article
Nanodroplet Processing Platform for Deep and Quantitative Proteome Profiling of 10–100 Mammalian Cells
Nanoscale or single-cell technologies are critical for biomedical applications. However, current mass spectrometry (MS)-based proteomic approaches require samples comprising a minimum of thousands of…Read article
How to Conduct Localized Proteomics of Microscopic Regions
In this webinar, you will learn: - How to perform isolated proteomics of microscopic regions of interest - How to use this technique with FFPE tissue - How proteomics can help you understand…Read article
Correlation of Phenotype and Genotype – microscopic samples and their biomolecular backgrounds
Seeing is believing, this is what makes microscopes, imaging systems, or box-type imagers so important for researchers in many different fields like pathology research, cancer research, neuroscience,…Read article
MicroRNA Expression Profiling to Identify and Validate Reference Genes for the Relative Quantification of microRNA in Rectal Cancer
Application example for laser microdissection (LMD) - MicroRNAs (miRNAs) play important roles in regulating biological processes at the post-transcriptional level. Deregulation of miRNAs has been…Read article
Workflows & Protocols: Plant Laser Microdissection
During Leica workshops for LMD users in Brazil, hosted by the Federal University of Paraná/UFPR (UFPR) at the Centro de Energia Nuclear na Agricultura/USP (CENA), the power of laser microdissection…Read article
Workflows & Protocols: Laser Microdissection for Pathology and Cancer Research
Tumor development results from mutations in our DNA. For their deeper analysis, cancer researchers have to dissect the relevant tissue areas. Here we report the reason why laser microdissection is a…Read article
Workflows & Protocols: Connecting Microscopy and Molecular Biology in Neuroscience
The main topic during this course was how to apply laser microdissection in neuroscience. Leica specialists demonstrated why laser microdissection is a suitable techniques for brain investigation as…Read article
Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection
During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome.…Read article
Workflows & Protocols: How to Use a Leica Laser Microdissection System and Qiagen Kits for Successful RNA Analysis
Laser Microdissection (LMD) allows isolating individual cells or chromosomes and is a well established technique for sample preparation prior downstream analysis of the nucleic acid content via PCR or…Read article
Consumables for Laser Microdissection
There are many different types of consumables for laser microdissection (LMD) systems. They cover a wide range of applications from basic to highly specialized, enabling scientists to choose their own…Read article
Total RNA Isolation after Laser-capture Microdissection of Human Cervical Squamous Epithelial Cells from Fresh Frozen Tissue
As most tissue specimens contain a mixture of different cell types including epithelial cells, stromal cells and immune cells, selection of the cells of interest is of utmost importance for the…Read article
Customized Patterned Substrates for Highly Versatile Correlative Light-Scanning Electron Microscopy
Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various…Read article
Identification of Molecular Pathways Facilitating Glioma Cell Invasion In Situ
Gliomas are mostly incurable secondary to their diffuse infiltrative nature. Thus, specific therapeutic targeting of invasive glioma cells is an attractive concept. As cells exit the tumor mass and…Read article
The Basic Principles of Laser Microdissection
In contrast to other systems which use a fixed laserfocus for dissection, Leica Microsystems' LMD systems guide the laserfocus for dissection. This unique feature allows highly precise laser…Read article
Glucose-Regulated Protein 78 (Grp78) Confers Chemoresistance to Tumor Endothelial Cells under Acidic Stress
This study was designed to investigate the activation of the unfolded protein response (UPR) in tumor associated endothelial cells (TECs) and its association with chemoresistance during acidic pH…Read article
Preparation of Laser Microdissected Plant Cell and Tissue Material for Microchemical Analysis
Secondary plant metabolites are involved in various plant physiological and biochemical processes, allowing plants to interact successfully with their biotic and abiotic environment. Distribution…Read article
The Morbus Parkinson Puzzle
A characteristic sign of M. Parkinson is the deterioration of dopaminergic neurons in the mid-brain, specifically in the substantia nigra (SN, black substance). Different causes and forms of this…Read article
Sorbin and SH3 Domain‐Containing Protein 2 Is Released from Infarcted Heart in the Very Early Phase: Proteomic Analysis of Cardiac Tissues From Patients
Many animal experiments have been performed to date to elucidate the mechanism of cardiac ischemic injury, and these studies have enhanced our understanding of cardiac remodeling following hypoxic…Read article
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal cord sections with Azure B in 70% ethanol.…Read article
Laser Microdissection (LMD) and Fancy Applications
New and far-reaching applications have recently been opened up in the field of laser microdissection. In addition to conventional dissection, the laser microdissection system (LMD) of Leica…Read article
Video: How to Achieve Fast Precise Cutting Lines in Laser Microdissection
This video explains the laser beam deflection principle of Leica Microsystems‘ laser microdissection systems (Leica LMD). The laser, the beam expander, motorized attenuator, motorized iris diaphragm…Read article
From Dynamic Live Cell Imaging to 3D Ultrastructure: Novel Integrated Methods for High Pressure Freezing and Correlative Light-Electron Microscopy
To correlate dynamic events in adherent cells with both ultrastructural and 3D information, we developed a method for cultured cells that combines confocal time-lapse images of GFP-tagged proteins…Read articleHistory of Laser Microdissection
Modern laser microdissection technology has its roots in the early 20th century. It has been steadily advanced and modified over the years. The following article is a summary of the history of laser…Read article
Useful Links
LMD Software
Video Tutorials on Leica LMD Software
Publication List
LMD reference list for major application fields in life science research
Communities and Web Sources
www.researchgate.net/ Social network for scientists
bitesizebio.com Online magazine and community for molecular and cell biology researchers
www.somersault1824.com Resource for high-end scientific illustrations, images and animations
Search Engines and Data Bases
www.cellimagelibrary.org Public resource database of images, videos, and animations of cells
harvester.fzk.de/harvester Bioinformatic meta search engine for genes and proteins
www.gopubmed.com Search interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_engines List of academic databases and search engines
scholar.google.com Beta of Google's search engine for scientific article abstracts
Journals
www.doaj.org/ Directory of open access journals
emboj.embopress.org/ The EMBO Journal
www.lifescied.org CBE-Life Sciences Education – an ASCB online journal
www.sciencemag.org/ Science
www.nature.com/ Nature
www.cell.com/ Biweekly publication of exceptional research articles
jcs.biologists.org/ Journal of Cell Science
dev.biologists.org/ Development
jeb.biologists.org/ The Journal of Experimental Biology
dmm.biologists.org/ DMM Disease Models & Mechanisms
www.biotechniques.com/ International Journal of Life Science Methods
www.opticsinfobase.org/ Collection of Journals and Proceedings in Optics and Photonics
spie.org/x576.xml SPIE - peer-reviewed journals on applied research in optics and photonics
onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648 Journal of Biophotonics
www.plosone.org/home.action International, peer-reviewed, open-access, online publication
rspb.royalsocietypublishing.org/ Proceedings B - the Royal Society's biological research journal
www.microscopy-analysis.com/ International Journal for microscopists
Organizations
www.microscopy.org/ Microscopy Society of America
www.eurmicsoc.org/ European Microscopy Society
www.rms.org.uk/ Royal Microscopical Society
www.ascb.org/ ASCB American Society of Cell Biology
www.biologists.com/cob_activities.html The company of biologists
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