Live Cell Imaging
With the variety of fluorescent proteins and multicolored probes that have been developed, it is now possible to label virtually any molecule. The ability to visualize protein dynamics in vesicles, organelles, cells, and tissues has provided new insights into how cells function in healthy and disease states. These insights include the spatiotemporal dynamics of processes like mitosis, embryonic development, and cytoskeleton changes.
When studying live cells, common obstacles include phototoxicity and photodamage. To capture fast biological processes, it is crucial to keep the cells healthy and obtain crisp images for reliable data that are free of artifacts. Live‐cell microscopy often requires a compromise between image quality and cell health. During imaging, certain environmental conditions must be maintained to avoid changes in the cells.
A variety of high-performance Leica imaging solutions can overcome these challenges for live-cell imaging enabling new information for cellular physiology and dynamics to be discovered.
Our experts on solutions for live cell imaging applications are happy to help you with their advice.
Cell Viability & Dynamics During Imaging
Leica Microsystems provides you with intelligent innovations in live-cell imaging. Our solutions help you get the best image quality while protecting your samples.
Most cellular processes occur in 3 dimensions over time. Therefore, cells need to be imaged in four dimensions (XYZ and time) to obtain a complete picture. Time‐lapse imaging is used to capture cell events over timescales from seconds to months. Repeated imaging of cells at particular points in time is also possible. To protect cell viability during this process, live-cell imaging requires the temperature, pH, and humidity to be kept under control. Light exposure should also be at a minimum to avoid phototoxicity.
Leica Microsystems offers imaging solutions that help optimize your study of live cells, even over long periods of time. They provide the necessary image contrast and resolution to facilitate the analysis of dynamic events. Some Leica systems also enable high-speed imaging, so no key cellular events are missed.
Your Live Cell Imaging Needs
To perform successful live-cell imaging experiments, using the right platform is critical. When choosing an optical microscope for live‐cell imaging, the following 3 variables should be considered: detector sensitivity (signal‐to‐noise ratio), specimen viability, and image-acquisition speed.
Methods suitable for live-cell applications enable visualization of the dynamics without causing cell damage, as it can affect the results.
Live‐cell imaging is mainly performed with fluorescence microscopy. Widefield microscopy, providing flexible excitation and fast acquisition, is typically used to visualize cell dynamics and development over long times. Confocal microscopy is typically used to study subcellular dynamic events. Multiphoton microscopy alllows excitation with longer wavelength light reducing photobleaching and extending cell viability. Finally, fluorescence lifetime imaging (FLIM) can be applied to study fast dynamic signaling events in cells.
Leica Microsystems offers the latest innovations in widefield and confocal imaging technologies for fast 3D live cell imaging with the THUNDER Imagers, STELLARIS confocal platform, and FLIM.
Live Cell Imaging Products
Filter by Area of Application
STELLARIS
With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.
K5
sCMOS Microscope Camera
THUNDER Imager Model Organism
The THUNDER Imager Model Organism allows fast and easy 3D exploration of whole organisms for developmental or molecular biology research.
STELLARIS DLS
Discover new applications by combining confocal and light-sheet microscopy
THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay
THUNDER Imagers provide you with a solution for advanced 3D cell culture assays, whether you want to study stem cells, spheroids, or organoids.
THUNDER Imager Tissue
The THUNDER Imager Tissue allows real-time fluorescence imaging of 3D tissue sections typically used in neuroscience and histology research.
PAULA
Smart Cell Imager
STELLARIS 8 FALCON
FLIM Microscope
LAS X Life Science
Software platform for life science applications
DMi8 S Platform
For routine to live cell research
Infinity TIRF
Multicolor advanced TIRF module
Leica DMi1
Entry level inverted microscope
Leica DM IL LED
Inverted Laboratory Microscope with LED Illumination
Leica DFC365 FX
1.4 MP cooled monochrome camera for standard live cell imaging of GFP-expressing cells and tissues
About Live Cell Imaging
Besides the structural organization of cells or organs, dynamic processes are a major contributor to a functioning biological entity. Naturally, these processes can be best observed in living cells with non-invasive techniques like optical methods, collectively called “live-cell imaging” methods. Live-cell imaging covers all techniques where live cells are observed with microscopes – from the observation of embryogenesis with stereo microscopes, via cell growth studies with compound microscopes, until studies of physiological states of cells or cellular transport using fluorescent dyes or proteins. Although being highly demanding for both, experimenter and equipment (e.g. imaging systems, climate control), live-cell imaging techniques deliver results that are indispensable for present-day research.
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THUNDER Imagers: High Performance, Versatility and Ease-of-Use for your Everyday Imaging Workflows
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Multiphoton Microscopy Publication List
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THUNDER Technology Note
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Confluency Check with PAULA Cell Imager
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Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images
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Solvent immersion imprint lithography
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Adipose Stem Cells Display Higher Regenerative Capacities and More Adaptable Electro-Kinetic Properties Compared to Bone Marrow-Derived Mesenchymal Stromal Cells
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Introduction to Mammalian Cell Culture
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Clap On, Clap Off. Protein On, Protein Off
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Chronic Inflammation Under the Microscope
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Super-Resolution Optical Microscopy of Lipid Plasma Membrane Dynamics
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