

Super-Resolution Microscopy
Sub-diffraction colocalization analysis reveals interactions unprecedented detail. Enabling novel discoveries to be made in the fields of virology, immunology, neuroscience and cancer, super-resolution is on its way to becoming the new gold standard in light microscopy.
Explore life’s true nature in unprecedented detail.
Contact a local specialist for advice on the right super-resolution or nanoscopy system for your needs.
Super-resolution and nanoscopy products
Filter by Area of Application

STELLARIS
With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth.

STELLARIS STED & STELLARIS 8 STED
STELLARIS brings the full power and potential of STED in a fully integrated system.
About Super-Resolution
It is about 20 years since super-resolution microscopy and nanoscopy arrived on the light microscopy scene, but it already plays an important role, particularly in life sciences – without superseding conventional confocal microscopy. The term super-resolution refers to methods that surpass the so-called diffraction limit. Applications are wide ranging – from dynamic vesicle movements to fluorescence images of sub-cellular structures, allowing researchers to see details in unprecedented detail. LIGHTNING Image Information Extraction is a super-resolution method that exploits the sub-diffraction lateral resolution capabilities of confocal microscopy. With LIGHTNING on a SP8 confocal microscope you can image multiple fluorophores simultaneously – without the need for sequential scanning. You can capture cellular details and observe dynamics with resolution down to 120 nm. As a truly infinitely super-resolving technology, STED nanoscopy offers resolution down to 30 nanometers. STED provides instant super-resolved imaging with multiple channels and approaching isotropic super-resolution in three dimensions. Underlining the impact of super-resolution microscopy, the 2014 Nobel Prize for Chemistry was awarded jointly to Eric Betzig, Stefan W. Hell and William E. Moerner "for the development of super-resolved fluorescence microscopy".
Related Articles
Time-resolved STED microscopy
Introduced more than 30 years ago, stimulated emission depletion (STED) microscopy has raised to a standard and widely used method for imaging in the life sciences. Thanks to continuous technological…Read articleMicroscopy in Virology
The coronavirus SARS-CoV-2, causing the Covid-19 disease effects our world in all aspects. Research to find immunization and treatment methods, in other words to fight this virus, gained highest…Read articleNanoscopy meets Lifetime: Introducing τ-STED
τ-STED combines the optical signals from STED and the photophysical information from the fluorescence lifetime at unprecedented speeds. This approach to STED uses phasor analysis in a novel way.…Read articleNew Light Shed on the Nanodomain Organization of the Endoplasmic Reticulum (ER)
The endoplasmic reticulum (ER) is a continuous membrane organelle in charge of protein synthesis, lipid synthesis and detoxification. The ER structure is described in terms of smooth peripheral…Read articleThe Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS SP8 STED 3X nanoscope from Leica Microsystems. It…Read articleExtending Nanoscopy Possibilities with STED and exchangeable fluorophores
When it comes to STED Nanoscopy, keeping high signal-to-noise is key to achieve the best possible resolution in fixed and living cells. This can be challenging in the case of experiments in 3D and/or…Read articleSee More Than Just Your Image
Despite the emergence of new imaging methods in recent years, true 3D resolution is still achieved by Confocal Laser Scanning Microscopy (CLSM). Through a combination of novel, extremely fast scanning…Read articleDynamic Endoplasmic Reticulum
The Endoplasmic Reticulum (ER) is a dynamic, reticular or net-like structure consisting of tubules and cisternae that extend throughout the cell and occupy a large portion of the cytoplasm. In recent…Read articleSimultaneously Measuring Image Features and Resolution in Live-Cell STED Images
Reliable interpretation and quantification of cellular features in fluorescence microscopy requires an accurate estimate of microscope resolution. This is typically obtained by measuring the image of…Read articleHow to extract Image Information by Adaptive Deconvolution
Confocal Laser Scanning Microscopy (CLSM) is the standard for true 3D resolved fluorescence imaging. Fast optical sectioning using flexible scanning strategies in combination with simultaneous…Read articleObserving Malaria Infection at the Right Spot in the Human Host
Malaria is a life-threatening disease transmitted through the bites of mosquitoes infected with protozoan parasites. The most common and dangerous type of malaria is caused by the parasite Plasmodium…Read articleSuper-resolved STED spectroscopy
Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules.Read articleSTED Nanoscopy at the forefront of cancer research
Alison Dun is the postdoctoral facility manager for the Edinburgh Super-Resolution Imaging Consortium (ESRIC), Heriot-Watt University, Edinburgh, UK. She has used a large range of microscope…Read articleResearchers Find a “Digital” Mechanism Behind Neuronal Changes from Learning
Neurons react to learning and memory by activating synaptic connections. The mechanisms behind this fundamental process are complex and poorly understood. Researchers at Thomas Jefferson University…Read articleBasal Body Positioning and Anchoring in the Multiciliated Cell Paramecium Tetraurelia: Roles of OFD1 and VFL3
The development of a ciliary axoneme requires the correct docking of the basal body at cytoplasmic vesicles or plasma membrane. In the multiciliated cell Paramecium, three conserved proteins, FOR20,…Read articleCalifornia NanoSystems Institute at UCLA Publications
A list of the published scientific articles which include work done in the ALMS/MSI Facilities.Read articleAbstracts of the 7th European Super-Resolution User-Club Meeting
The 7th Super-Resolution User Club Meeting was held in collaboration with Prof Pavel Hozák , at the Institute of Molecular Genetics of the ASCR in Prague. Keeping the event close to science is one of…Read articleAxial Tubule Junctions Control Rapid Calcium Signaling in Atria
The canonical atrial myocyte (AM) is characterized by sparse transverse tubule (TT) invaginations and slow intracellular Ca2+ propagation but exhibits rapid contractile activation that is susceptible…Read articleThe Molecular Architecture of Hemidesmosomes as Revealed by Super-Resolution Microscopy
Hemidesmosomes have been extensively studied by immunofluorescence microscopy, but due to its limited resolution, their precise organization remained poorly understood. We studied hemidesmosome…Read articleActin-Dependent Vacuolar Occupancy of the Cell Determines Auxin-Induced Growth Repression
The cytoskeleton is an early attribute of cellular life, and its main components are composed of conserved proteins. The actin cytoskeleton has a direct impact on the control of cell size in animal…Read articleThe Bif-1-Dynamin 2 Membrane Fission Machinery Regulates Atg9-Containing Vesicle Generation at the Rab11-Positive Reservoirs
Application example of HyVolution Super-Resolution - Atg9 is a multispanning transmembrane protein that is required for autophagosome formation. During autophagy, vesicles containing Atg9 are…Read articleQuantitative Analysis of PPT1 Interactome in Human Neuroblastoma Cells
Application example of HyVolution Super-Resolution - Mutations in the CLN1 gene that encodes Palmitoyl protein thioesterase 1 (PPT1) or CLN1, cause Infantile NCL (INCL, MIM#256730). PPT1 removes long…Read articleSuper-Resolution Optical Microscopy of Lipid Plasma Membrane Dynamics
Plasma membrane dynamics are an important ruler of cellular activity, particularly through the interaction and diffusion dynamics of membrane-embedded proteins and lipids.