Super-Resolution Microscopy
Sub-diffraction colocalization analysis reveals interactions unprecedented detail. Enabling novel discoveries to be made in the fields of virology, immunology, neuroscience and cancer, super-resolution is on its way to becoming the new gold standard in light microscopy.
Explore life’s true nature in unprecedented detail.
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Leica TCS SP8 STED
STED Nanoscopes
About Super-Resolution
It is about 20 years since super-resolution microscopy and nanoscopy arrived on the light microscopy scene, but it already plays an important role, particularly in life sciences – without superseding conventional confocal microscopy. The term super-resolution refers to methods that surpass the so-called diffraction limit. Applications are wide ranging – from dynamic vesicle movements to fluorescence images of sub-cellular structures, allowing researchers to see details in unprecedented detail. LIGHTNING Image Information Extraction is a super-resolution method that exploits the sub-diffraction lateral resolution capabilities of confocal microscopy. With LIGHTNING on a SP8 confocal microscope you can image multiple fluorophores simultaneously – without the need for sequential scanning. You can capture cellular details and observe dynamics with resolution down to 120 nm. As a truly infinitely super-resolving technology, STED nanoscopy offers resolution down to 30 nanometers. STED provides instant super-resolved imaging with multiple channels and approaching isotropic super-resolution in three dimensions. Underlining the impact of super-resolution microscopy, the 2014 Nobel Prize for Chemistry was awarded jointly to Eric Betzig, Stefan W. Hell and William E. Moerner "for the development of super-resolved fluorescence microscopy".
New Light Shed on the Nanodomain Organization of the Endoplasmic Reticulum (ER)
The endoplasmic reticulum (ER) is a continuous membrane organelle in charge of protein synthesis, lipid synthesis and detoxification. The ER structure is described in terms of smooth peripheral…Read article
The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the TCS SP8 STED 3X nanoscope from Leica Microsystems. It…Read article
Extending Nanoscopy Possibilities with STED and exchangeable fluorophores
When it comes to STED Nanoscopy, keeping high signal-to-noise is key to achieve the best possible resolution in fixed and living cells. This can be challenging in the case of experiments in 3D and/or…Read article
See More Than Just Your Image
Despite the emergence of new imaging methods in recent years, true 3D resolution is still achieved by Confocal Laser Scanning Microscopy (CLSM). Through a combination of novel, extremely fast scanning…Read article
Dynamic Endoplasmic Reticulum
The Endoplasmic Reticulum (ER) is a dynamic, reticular or net-like structure consisting of tubules and cisternae that extend throughout the cell and occupy a large portion of the cytoplasm. In recent…Read article
Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images
Reliable interpretation and quantification of cellular features in fluorescence microscopy requires an accurate estimate of microscope resolution. This is typically obtained by measuring the image of…Read article
How to extract Image Information by Adaptive Deconvolution
Confocal Laser Scanning Microscopy (CLSM) is the standard for true 3D resolved fluorescence imaging. Fast optical sectioning using flexible scanning strategies in combination with simultaneous…Read article
Observing Malaria Infection at the Right Spot in the Human Host
Malaria is a life-threatening disease transmitted through the bites of mosquitoes infected with protozoan parasites. The most common and dangerous type of malaria is caused by the parasite Plasmodium…Read article
Super-resolved STED spectroscopy
Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules.Read article
STED Nanoscopy at the forefront of cancer research
Alison Dun is the postdoctoral facility manager for the Edinburgh Super-Resolution Imaging Consortium (ESRIC), Heriot-Watt University, Edinburgh, UK. She has used a large range of microscope…Read article
Researchers Find a “Digital” Mechanism Behind Neuronal Changes from Learning
Neurons react to learning and memory by activating synaptic connections. The mechanisms behind this fundamental process are complex and poorly understood. Researchers at Thomas Jefferson University…Read article
Basal Body Positioning and Anchoring in the Multiciliated Cell Paramecium Tetraurelia: Roles of OFD1 and VFL3
The development of a ciliary axoneme requires the correct docking of the basal body at cytoplasmic vesicles or plasma membrane. In the multiciliated cell Paramecium, three conserved proteins, FOR20,…Read article
California NanoSystems Institute at UCLA Publications
A list of the published scientific articles which include work done in the ALMS/MSI Facilities.Read article
Abstracts of the 7th European Super-Resolution User-Club Meeting
The 7th Super-Resolution User Club Meeting was held in collaboration with Prof Pavel Hozák , at the Institute of Molecular Genetics of the ASCR in Prague. Keeping the event close to science is one of…Read article
Axial Tubule Junctions Control Rapid Calcium Signaling in Atria
The canonical atrial myocyte (AM) is characterized by sparse transverse tubule (TT) invaginations and slow intracellular Ca2+ propagation but exhibits rapid contractile activation that is susceptible…Read article
The Molecular Architecture of Hemidesmosomes as Revealed by Super-Resolution Microscopy
Hemidesmosomes have been extensively studied by immunofluorescence microscopy, but due to its limited resolution, their precise organization remained poorly understood. We studied hemidesmosome…Read article
Actin-Dependent Vacuolar Occupancy of the Cell Determines Auxin-Induced Growth Repression
The cytoskeleton is an early attribute of cellular life, and its main components are composed of conserved proteins. The actin cytoskeleton has a direct impact on the control of cell size in animal…Read article
The Bif-1-Dynamin 2 Membrane Fission Machinery Regulates Atg9-Containing Vesicle Generation at the Rab11-Positive Reservoirs
Application example of HyVolution Super-Resolution - Atg9 is a multispanning transmembrane protein that is required for autophagosome formation. During autophagy, vesicles containing Atg9 are…Read article
Quantitative Analysis of PPT1 Interactome in Human Neuroblastoma Cells
Application example of HyVolution Super-Resolution - Mutations in the CLN1 gene that encodes Palmitoyl protein thioesterase 1 (PPT1) or CLN1, cause Infantile NCL (INCL, MIM#256730). PPT1 removes long…Read article
Super-Resolution Optical Microscopy of Lipid Plasma Membrane Dynamics
Plasma membrane dynamics are an important ruler of cellular activity, particularly through the interaction and diffusion dynamics of membrane-embedded proteins and lipids. FCS (fluorescence…Read article
Methods to Calibrate and Scale Axial Distances in Confocal Microscopy as a Function of Refractive Index
Application example of HyVolution Super-Resolution - Accurate distance measurement in 3D confocal microscopy is important for quantitative analysis, volume visualization and image restoration.…Read article
Evaluation of Zebrafish as a Model to Study the Pathogenesis of the Opportunistic Pathogen Cronobacter Turicensis
Application example of HyVolution Super-Resolution - Bacteria belonging to the genus Cronobacter spp. have been recognized as causative agents of life-threatening systemic infections, primarily in…Read article
Discovery of Novel Peptides Targeting Pro-Atherogenic Endothelium in Disturbed Flow Regions -Targeted siRNA Delivery to Pro-Atherogenic Endothelium in vivo
Application example of HyVolution Super-Resolution - Atherosclerosis occurs preferentially in arterial regions exposed to disturbed blood flow. Targeting these pro-atherogenic regions is a potential…Read article
Practical Guide for Excellent GSDIM Super-Resolution Images
Do you know that most protists and bacteria lack in one feature that each of our body cell has? Our cells are touch and communicate with one another. They send and receive a variety of signals that…Read article
Initiation of Lamellipodia and Ruffles Involves Cooperation Between mDia1 and the Arp2/3 Complex
Protrusion of lamellipodia and ruffles requires polymerization of branched actin filaments by the Arp2/3 complex. Although regulation of Arp2/3 complex activity has been extensively investigated, the…Read article
Visualizing Tropoelastin in a Long-Term Human Elastic Fibre Cell Culture Model
Elastin is an essential protein found in a variety of tissues where resilience and flexibility are needed, such as the skin and the heart. When aiming to engineer suitable implants, elastic fibres are…Read article
P53- and Mevalonate Pathway–Driven Malignancies Require Arf6 for Metastasis and Drug Resistance
Application example of HvYolution Super-Resolution - Drug resistance, metastasis, and a mesenchymal transcriptional program are central features of aggressive breast tumors. The GTPase Arf6, often…Read article
Botulinum Neurotoxin Type-A Enters a Non-Recycling Pool of Synaptic Vesicles
Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been…Read article
Abstracts of the 6th European Super-Resolution User-Club Meeting
The 6th European Super-Resolution User Club Meeting was held in collaboration with Dr. Timo Zimmermann, CRG, and Dr. Pablo Loza-Alvarez, ICFO, Barcelona. According to the founding principle of the…Read article
Measuring the 3D STED-PSF with a new Type of Fluorescent Beads
A new type of fluorescent bead is presented by GATTAquant. These beads, called GATTA-Beads, are characterized by a small diameter (23 nm), high intensity and size uniformity. In combination with…Read article
Useful Links
Super-Resolution & Nanoscopy Publications
Communities and Web Sources
www.researchgate.net/Social network for scientists
www.lsoft.com/scripts/wl.exe?SL1=CONFOCALMICROSCOPY&H=LISTS.UMN.EDUConfocal Microscopy Mailing List, University of Minnesota
www.ibiology.org/Teaching tools, video lectures on biology and microscopy
bitesizebio.comOnline magazine and community for molecular and cell biology researchers
Search Engines and Data Bases
www.cellimagelibrary.orgPublic resource database of images, videos, and animations of cells
harvester.fzk.de/harvesterBioinformatic meta search engine for genes and proteins
www.gopubmed.comSearch interface for pubmed
en.wikipedia.org/wiki/List_of_academic_databases_and_search_enginesList of academic databases and search engines
scholar.google.comBeta of Google's search engine for scientific article abstracts
Journals
www.optnano.com/Optical Nanoscopy
www.doaj.org/Directory of open access journals
emboj.embopress.org/The EMBO Journal
www.lifescied.orgCBE-Life Sciences Education – an ASCB online journal
www.sciencemag.org/Science
www.nature.com/Nature
www.cell.com/Biweekly publication of exceptional research articles
jcs.biologists.org/Journal of Cell Science
dev.biologists.org/Development
jeb.biologists.org/The Journal of Experimental Biology
dmm.biologists.org/DMM Disease Models & Mechanisms
www.biotechniques.com/International Journal of Life Science Methods
www.opticsinfobase.org/Collection of Journals and Proceedings in Optics and Photonics
spie.org/x576.xmlSPIE - peer-reviewed journals on applied research in optics and photonics
onlinelibrary.wiley.com/journal/10.1002/(ISSN)1864-0648Journal of Biophotonics
www.plosone.org/International, peer-reviewed, open-access, online publication
rspb.royalsocietypublishing.org/Proceedings B - the Royal Society's biological research journal
www.microscopy-analysis.com/International Journal for microscopists
Organizations
www.microscopy.org/Microscopy Society of America
www.eurmicsoc.org/European Microscopy Society
www.rms.org.uk/Royal Microscopical Society
www.ascb.org/ASCB American Society of Cell Biology
www.biologists.com/cob_activities.htmlthe company of biologists
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