Advancing Cell Biology with Cryo-Correlative Microscopy


Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with cryo-electron tomography allows investigators to bridge knowledge gaps between cell and structural biology. CLEM technologies are rapidly advancing in order to improve biological understanding, experimental reproducibility, and automation. Throughout the webinar, we will highlight insights that have been gleaned in several biological systems studied using CLEM workflows. We will review some of the basics of the technologies and provide examples of optimal workflows. We will also discuss some of the procedures for cell care, patterning substrates, culturing cells on substrates, and cryo-CLEM imaging.


Elizabeth R. Wright, Ph.D.

Professor of Biochemistry; Affiliate, Morgridge Institute for Research University of Wisconsin-Madison (USA)

Bio: Liz received her Ph.D. in Chemistry from Emory University. She engineered elastin-mimetic materials that are used for drug delivery and tissue engineering applications. She was a postdoctoral research associate in materials science at the University of Southern California. She was a postdoctoral scholar with Professor Grant Jensen at Caltech where she developed cryo-ET technologies and used cryo-ET to study HIV-1 maturation. She joined Emory University as an Assistant Professor in 2008 and was promoted to Associate Professor in 2016. She moved to the University of Wisconsin, Madison as a full Professor in 2018. Her research program focuses on the development and use of cryo-EM and correlative light and electron microscopy (CLEM) imaging technologies to determine the native-state structures of several bacterial species, bacteriophages, HIV-1, respiratory syncytial virus (RSV), measles virus (MeV), and other host-pathogen systems.

Jae E. Yang, Ph.D.

Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA).

Bryan S. Sibert, Ph.D.

Postdoctoral Research Associate, Wright Laboratory, University of Wisconsin-Madison (USA)

Joseph Y. Kim

Graduate Student, Wright Laboratory University of Wisconsin-Madison (USA)

Key Learnings

  • Discussion of results from infectious disease and neuroscience research.
  • Review of the equipment and techniques necessary for CLEM success in cell biology.
  • Summarize how to define and optimize important parameters to improve results, including Tips & Tricks.

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