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Zebrafish Research

Taking substantiated decisions in Zebrafish research. For the best result during screening, sorting, manipulation, and imaging you need to see details and structures to make the right decisions for your next steps in research. Known for outstanding optics and superb resolution, stereo microscopes and transmitted light bases from Leica are the preferred choice of researchers worldwide.

It’s all about high resolution, high color fidelity and optimized contrasting leading to insightful decisions.

Simply get in touch!

Please contact us for a demo request or personal expert advice on our microscopy solutions for zebrafish research.

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The basis for correct decisions in Screening & Sorting

Do you struggle to see finest pigments and structural differences in your zebrafish? The identification of the right phenotype is crucial and can be demanding.

See more details at first glance – Leica zebrafish screening solutions allow you to recognize finest structures and more colors even at low magnification. This means clear understanding of the nervous system, heart, blood vessels, and pigmentation.

Improve your work experience by

  • Easy selection of the perfect contrast
  • Homogenous contrast over the entire FOV
  • Steady contrast without readjusting over the entire zoom range

Make a difference in your screening & sorting with the Leica C.Elegans screening solution: M165 C and TL3000 Ergo.

Fascinating information with different contrast options

Get the full color spectrum of your staining with the specially designed LEDs in the Leica TL bases.

  • Evaluate staining and see realistic colors of your specimen in bright field
  • Investigate internal structures with Rottermann Contrast
  • Explore the smallest details with dark field illumination

Fluorescence Screening: Don’t let background noise mask your signals

Detecting xFP fusion proteins can be very challenging. You try to keep the expression physiologically low for realistic protein levels. Signals are therefore faint and hard to identify.

In this situation your microscope solution should not be working against you with high levels of autofluorescence, e.g. from lens glue, glass, and the LED of your microscope base.

We solve these problems with

  • A separate excitation channel in our fluorescence stereo microscopes, known as Triple Beam
  • A specially designed mirror in our screening bases that doesn’t let autofluorescence come back
  • Adjustable flaps covering the LED in the automated base to block autofluorescence during imaging

This results in a clear and strong fluorescence signal against a noise-free, black background.

Manipulate your specimen with ease

Leica stereo microscopes allow easy orientation in 3D. This ensures that you see exactly where your tools are, so you don’t accidently injure your model organism. 

Our zebrafish solutions enable you to

  • Manipulate easily with a natural hand-eye-coordination 
  • Set injections and lesions precisely
  • Position your sample ideally for imaging

The perfect finish – publishable results

Functional imaging is one of the most demanding tasks in the laboratory. Leica supports you with fully automated stereo microscopes, like the M205 FA.

Work completely software based and control

  • Fluorescence light, filters, and shutter
  • Transmitted light contrast 
  • x/y/z-positions
  • Camera
  • Magnification
  • Environment

With the fully automated TL5000 Ergo transmitted light base from Leica, functional imaging brings out the best of your fish. It provides highest NA of 0.9 and adjusts the aperture automatically to the zoom, in order to eliminate stray light.

Zebrafish related Science Lab articles

  • High-Resolution 3D Imaging of Whole Organ after Clearing

    Zebrafish testis has become a powerful model for reproductive biology of teleostean fishes and other vertebrates and encompasses multiple applications in applied and basic research. Many studies have focused on 2D images, which is time consuming and implies extrapolation of results. Three-dimensional imaging of whole organs recently became an important challenge to better understand their architecture and allow cell enumeration.
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  • DLS Sample Preparation: Using U-Shaped Glass Capillaries for Sample Mounting

    The TCS SP8 DLS microscope system from Leica Microsystems is an innovative concept which integrates the Light Sheet Microscopy technology into the SP8 confocal platform. Due to its unique optical architecture, samples can be mounted on standard glass bottom petri dishes and require little or no adaptation when compared to conventional mounting procedures. Here, we present a convenient way to prepare specimens quickly for light sheet imaging with the TCS SP8 DLS system.
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  • Real Time Observation of Neutrophil White Blood Cell Recruitment to Bacterial Infection In Vivo

    The zebrafish (Danio rerio) is an emerging vertebrate model organism to study infection. The transparent larva comprises a fully functional innate immune system and enables live imaging of fluorescent immune cells in transgenic animals. Zebrafish infection models have been developed for both the human bacterial pathogen Shigella flexneri and the natural fish bacterial pathogen Mycobacterium marinum. Importantly, whilst S. flexneri causes acute infection and is typically used as an inflammatory paradigm, M. marinum causes a chronic disease similar to tuberculosis in humans. Here, we use real time fluorescence microscopy to image transgenic zebrafish larvae with neutrophils (granulocyte white blood cells) expressing the green fluorescent protein eGFP.
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  • Evaluation of Zebrafish as a Model to Study the Pathogenesis of the Opportunistic Pathogen Cronobacter Turicensis

    Application example of HyVolution Super-Resolution - Bacteria belonging to the genus Cronobacter spp. have been recognized as causative agents of life-threatening systemic infections, primarily in premature, low-birth weight and/or immune-compromised neonates. Knowledge remains scarce regarding the underlying molecular mechanisms of disease development. In this study, we evaluated the use of a zebrafish model to study the pathogenesis of Cronobacter turicensis LMG 23827T, a clinical isolate responsible for two fatal sepsis cases in neonates.
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  • Work More Efficiently in Developmental Biology With Stereo Microscopy: Zebrafish, Medaka, and Xenopus

    Among the aquatic model organisms used in molecular and developmental biology the most prominent are the zebrafish (genus species: Danio rerio), medaka or japanese rice fish (genus species: Oryzias latipes), and african clawed frog (genus species: Xenopus laevis). This report gives useful information to scientists and technicians which can help improve their daily laboratory work by making the steps of transgenesis, fluorescent screening, and functional imaging more efficient.
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  • Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens

    Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella typhimurium and Mycobacterium marinum. Micro-injection of bacteria and multi-color fluorescence imaging are essential techniques involved in the application of zebrafish embryo infection models.
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  • Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

    We describe a technique of microinjecting the aminoglycoside, gentamicin, into 2 days post-fetilization (dpf) zebrafish larvae to induce acute kidney injury (AKI). We also describe a method for whole mount immunohistochemistry, plastic embedding and sectioning of zebrafish larvae to visualize the AKI mediated damage.
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  • Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells

    Mauthner cells (M-cells) are large reticulospinal neurons located in the hindbrain of teleost fish. They are key neurons involved in a characteristic behavior known as the C-start or escape response that occurs when the organism perceives a threat. The M-cell has been extensively studied in adult goldfish where it has been shown to receive a wide range of excitatory, inhibitory and neuromodulatory signals. We have been examining M-cell activity in embryonic zebrafish in order to study aspects of synaptic development in a vertebrate preparation. In the late 1990s Ali and colleagues developed a preparation for patch clamp recording from M-cells in zebrafish embryos, in which the CNS was largely intact.
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  • Organ Regeneration: An Unlikely Fish Tale

    Spectacular discoveries in cardiac tissue regeneration are rapidly moving researchers closer to the goal of harnessing regenerative techniques to repair the human heart. Only eleven years ago, Dr. Kenneth Poss, Professor of Cell Biology at Duke University and an Early Career Scientist of the Howard Hughes Medical Institute, published the first research to clearly visualize an example of cardiac tissue regeneration using fluorescence microscopy.
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  • Find the Needle in the Haystack

    The obvious has been explored. These days, biologists strive to identify and analyze hidden and rare events. The task is tackled by automatically screening large numbers of objects – typically growing in multi-well plates – over a long period of time. When an interesting feature is identified (manually or by means of computed recognition), modern systems can automatically monitor and record these events at high resolution.
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  • Deep Tissue Imaging

    Developmental biology using Multiphoton microscopy with OPO. To gain new insight into the fundamental control of cell response to physical changes and to study the dynamics and roles of biological flow during the development of the zebrafish, Dr. Julien Vermot established his lab last year at the Institute of Genetics and Molecular and Cellular Biology (IGBMC) in Strasbourg, France.
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