This course is centered around biological applications that can benefit how lifetime-based information can provide new functional and mechanistic insights to biological questions.
Topics covered
- The basics of fluorescence lifetime and the additional dimension it can provide to imaging experiments
- Measuring protein-protein interactions
- Environmental sensing with FRET-based and non-FRET-based biosensors
- Using fluorescence lifetime for multiplexing
- Increasing the resolution with TauSTED on STELLARIS 8 STED FALCON
- Understand the basis of endogenous fluorescence (in particular in tissue and animal models) and how fluorescence lifetime can help to exploit or cope with their signals, e.g. by combining multiphoton imaging and endogenous lifetime information to study metabolic changes on STELLARIS 8 DIVE FALCON
- Sessions at the microscopes to allow all participants to practice using lifetime imaging in exemplary workflows
- Analysis of the lifetime-based information and applicative conclusions
Registration is open until April 17. If you are interested to participate or would like to share with others, you can find more details on the course program and the registration form on the official course page:
https://www.embl.org/about/info/course-and-conference-office/events/mic23-03/
