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Fluorescence Correlation Spectroscopy (FCS)

Fluorescence correlation spectroscopy (FCS) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as the diffusion time, number of molecules or dark…

Live cell imaging, 4 colors: Mitochondria (MitoView Green, yellow) and actin (mNeonGreen, cyan) microtubuli (SIR-tubulin, magenta), endosomes (NIR750, green). Processed with DSE and DSE powered by Aivia.

The Principles of White Light Laser Confocal Microscopy

The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime fluorescence. Furthermore, it should offer means…

Förster Resonance Energy Transfer (FRET)

The Förster Resonance Energy Transfer (FRET) phenomenon offers techniques that allow studies of interactions in dimensions below the optical resolution limit. FRET describes the transfer of the energy…

An Introduction to CARS Microscopy

CARS overcomes the drawbacks of conventional staining methods by the intrinsic characteristics of the method. CARS does not require labeling because it is highly specific to molecular compounds which…

Scheme of a 2D mosaic scan. Drosophila melanogaster (eye section)

Mosaic Images

Confocal laser scanning microscopes are widely used to create highly resolved 3D images of cells, subcellular structures and even single molecules. Still, an increasing number of scientists are…

Confocal Optical Section Thickness

Confocal microscopes are employed to optically slice comparably thick samples.