Overcome the first challenges of the cryo-ET sample preparation process by using PRIMO, a micropatterning system that allows control of cell adhesion, spreading and shape on EM grids. It enables precise cell positioning and optimized cell spreading, while leaving the surface of the EM grid undamaged. The software automatically aligns your patterns within the mesh of your EM grid, ensuring that your cells are optimally positioned for further processing.

Learn more about PRIMO: https://www.alveolelab.com/our-products/primo-micropatterning/

Prior to freezing, the sample is maintained in an humidity-controlled environmental chamber at a stable temperature.

The sample is then vitrified by plunge freezing with the automatic plunge freezer EM GP2. The vitrification process inhibits ice crystal formation ensuring the cellular content is as close to the native state as possible.

For an efficient workflow, suitable cells and target regions have to be preselected. This is achieved by using the cryo light microscope THUNDER Imager EM Cryo CLEM. The sample can then be transferred to the Thermo Scientific™ Aquilos™ for milling. Full connectivity between the instruments means that the defined region and coordinates are securely and precisely transferred for immediate relocation by the Aquilos. No need to invest time searching for the relevant target positions!

To avoid contamination of the precious specimen, the Integrated Cryo Tomography workflow ensures a secure transfer of the sample between the systems involved. The dedicated cartridge system safeguards your sample throughout the workflow providing the solid fundament for your reliable scientific results.

(Thermo Scientific, Krios and Aquilos are Trademarks by Thermo Fisher Scientific.)