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The Shape of the Brain: Spatial Biology of Alzheimer’s Disease

Using Cell DIVE multiplexed imaging and antibodies from CST to uncover the architecture of the brain in the context of neurodegeneration

Adult human Alzheimer’s brain demonstrating a panel of 15 markers. Adult_human_Alzheimers_brain.jpg

Alzheimer’s disease (AD) is a neurodegenerative disease and a common cause of cognitive impairment acquired in later life. AD is defined by the presence of β-amyloid-containing plaques and phosphorylated tau containing neurofibrillary tangles. Effective therapies for the treatment and prevention of AD are lacking. We used Cell DIVE in combination with antibodies from Cell Signaling Technology to examine synaptic processes and spatially define cells, such as glia and neurons, in AD and demonstrate that multiplexed imaging can be used to probe AD brain. Download this scientific poster to learn how cell-type markers combined with multiplexed imaging provides a new way for researchers to understand spatial heterogeneity of the brain and its contribution to neurodegenerative disease. 

Key Learnings

What to expect from the poster

  • Learn how Cell DIVE multiplexed imaging can be used to explore human brain samples
  • See how antibodies from Cell Signaling Technology can accelerate multiplexed imaging workflow
  • Understand how cell identity and spatial heterogeneity of the brain tissue contribute to the structure of the Alzheimer’s brain

About Alzheimer's disease

Alzheimer's disease (AD) is the most common type of dementia causing cognitive impairment in midlife and late life. It is a neurodegenerative disorder with both genetic and non-genetic causes. AD is characterized by the presence of β-amyloid-containing plaques and phosphorylated tau neurofibrillary tangles. Current treatments for AD are limited, and researchers are investigating the role of histopathological features for potential therapeutic approaches. The Cell DIVE Multiplexed Imaging Solution, along with CST's antibodies, provides a way to study synaptic processes and identify cell types around AD hallmarks.

The study

This approach helps to recognize disease-related microglia and comprehend cellular relationships that contribute to AD. This method identifies spatially co-localized cell populations and serves as a useful tool for exploring AD. CST's many antibodies can help spot changes in the way cells communicate and different cell types in diseased tissue. With a novel CST panel, researchers can examine the protein landscape and look at how Amyloid-β and tau are expressed in people with Alzheimer's.

CST's antibodies are subjected to rigorous validation, so they work well on tissue that has been preserved with formalin and buried in paraffin. The study's direct connections were repeatedly colored, giving a description of the spatial makeup of AD brain tissue. The tissue-preserving nature of Cell DIVE enables deeper investigation, presenting a new technique for neuroscience to understand the differences in space in the human brain and their connection to illness. Targeted studies of certain brain regions, such as the amygdala, could reveal fresh spatial features linked to AD, creating new paths for future therapeutic approaches.

Download the Poster

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