Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches
On-demand webinar by Dr. Sergi Padilla-Parra & Dr. Zhongxiang Jiang
Understanding molecular interactions in living cells is crucial for deciphering molecular mechanisms underlying most cellular functions. The gold standard for studying protein-protein interaction is Förster resonance energy transfer (FRET). Although there are several approaches to demonstrate FRET in biological samples, using fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.
In this Webinar, Dr. Padilla-Parra shows how much information you can harness from FRET. He explains how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time. He also talks about some of the limitations of classical FRET approaches.
After the presentation, Dr. Zhongxiang Jiang gives a live showcase at the STELLARIS 5 confocal system with an integrated White Light Laser (WLL), combined with the proprietary Acousto-optical Beam Splitter (AOBS) and new Power HyD S detectors. He shows the straightforward application of lifetime-based non-fitting FRET using the new and unique TauSense technology.
- how to investigate protein-protein interaction in live samples;
- ways to get the best out of FRET using fluorescence lifetime information;
- how non-fitting approaches can be powerful tools for functional imaging;
- what the minimal fraction of interacting donors (mFD) can tell you about molecular interactions in cells;
- practical insights into using the STELLARIS 5 confocal system.