ATTO 542 DPPE Labeled Phospholipid 1 mg
Product No. AD-542-151
ATTO-Dye Labeled Phospholipids
In recent years the investigation of biological membranes, e.g. intracellular membranes of live cells, plasma membranes etc., has become a major subject of interest. As a result there is a growing demand for fluorescent dye-labeled lipids, in particular glycero-phospholipids, to be incorporated in biological membranes. ATTO-TEC offers a variety of glycero-phospholipids, carrying one or two fatty acid chains (lipophilic groups) and a phosphate ester residue (hydrophilic group).
ATTO-dye fluorescent phospholipids are labeled at the hydrophilic head group. After incorporation in, e.g., a plasma membrane (phospholipid bilayer) the fluorophore is located at the water/lipid interface of the membrane.
"ATTO-TEC is a wholly owned subsidiary of Leica Microsystems"
Currently we provide the phospholipids
- 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE),
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE),
- 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine (PPE)
- 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), and
- 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE)
labeled with ATTO-dyes.
ATTO 542
ATTO 542 is a new fluorescent label related to ATTO 532.
The new dye is very hydrophilic and shows excellent water solubility. Characteristic features of the label are strong absorption and high photo-stability. The dye shows a very high fluorescence quantum yield even after coupling to a biomolecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy (PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.
After coupling to a substrate the dye carries a net electrical charge of - 3. The fluorescence is excited most efficiently in the range 520 - 555 nm.
