Viventis SCAPE Single Objective Light Sheet Microscope
ESCAPE the limits of 3D live cell imaging with single objective light sheet microscopy. The Viventis SCAPE microscope is based on swept confocally aligned planar excitation (SCAPE) microscopy designed for gentle and fast volumetric imaging with standard sample carriers.
ESCAPE to fast volumetric imaging and higher throughput
Volumetric imaging without moving the sample is enabled by SCAPE scanning.
This delivers extremely fast 3D acquisition. You can follow rapid signaling processes in three dimensions as they unfold, ensuring that all relevant information is captured.
Alternatively, it supports higher throughput by enabling more samples to be imaged in less time.
ESCAPE phototoxicity and photobleaching
By illuminating only the imaging plane, Viventis SCAPE minimizes light exposure outside the focal region, reducing phototoxicity and photobleaching. This gentle illumination approach enables rapid and prolonged volumetric imaging of light-sensitive samples.
Low endogenous expression of fluorescently tagged proteins can make it challenging to capture biological processes. Viventis SCAPE’s gentle, high-sensitive imaging allows you to follow these processes in 3D over extended periods, revealing dynamic events that might otherwise remain undetected.
ESCAPE the need for special sample mounting
With its underlying oblique plane illumination (OPM) technology and inverted configuration, Viventis SCAPE helps you to image samples mounted in standard flat-bottom carriers. Without requiring specialized sample preparation, the technique is accessible and easier than ever to integrate into existing workflows.
Viventis SCAPE supports multi well plates with up to at least 96 wells. When combined with rapid volumetric imaging, it enables higher throughput and faster time to results.
Range of applications
Viventis SCAPE supports a wide range of applications, including 2D adherent cell cultures, tissue slices, 3D cell cultures, as well as larger embryos and small model organisms.
While the accessible z-volume of the sample is defined by the objective´s long working distance (620 µm), the accessible x-dimension is constrained only by the stage travel range. Large fields of view can be achieved through tiled acquisitions.
Crisp images free of artifacts
Auto alignment
With a single mouse-click, the optimal light sheet setting provides you with sharp, high-resolution images.
Beam Pivoting
As light sheet imaging is prone to stripe-artifacts caused by sample scattering, beam pivoting illuminates the sample from multiple angles. This reduces stripe artifacts for cleaner, and more uniform images.
Experience your data as expected
On-the-fly deskewing
Single objective light sheet imaging produces an oblique view that can make navigation and analysis challenging. With Slice of Life, Viventis SCAPE transforms this oblique view into a familiar, conventional xyz-perspective – making sample navigation intuitive. Realtime deskewing and reslicing during acquisition ensure that your data is delivered in a familiar, widefield-like format, and is ready for immediate analysis.
Computational clearing
Viventis SCAPE-optimized computational clearing and LIGHTNING enable you to obtain images with enhanced contrast and resolution.
Enabling your success with complete workflow support
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Key features
- Leica Team: 500+ Service & Application experts
- Leica Training: 4-level factory certification program
- Leica Logistics: 5 regional hubs for genuine parts
- Leica OneCall: PhD-level hotline assistance
