High pressure freezing is often the preferred method for preserving aqueous samples in their close-to-native state, as it captures the intricate changes in fine structure or cellular dynamics. Leica Microsystems combines high pressure freezing with light stimulation or electrical stimulation: It enables you to visualize highly dynamic processes or the structural changes of samples at a nanometer resolution and with millisecond precision. This allows researchers in life sciences and industry to get answers to questions for which they were unable to design experiments before.
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The Leica EM ICE high pressure freezer is the only instrument capable of synchronizing freezing and stimulation with a millisecond precision. Apply light stimulation to any light sensitive compound such as lotions, cosmetics, or food, and photo-activated samples such as proteins, or various biological samples.
To prepare vitrified thin samples for cryo-TEM, including biological suspensions and industrial emulsions in both aqueous and inorganic solvents, the bare grid technique is used. The Leica EM GP automatic plunge freezer allows you to plunge freeze samples under defined environmental and blotting settings for perfect imaging results.
Freeze Substitution is a common follow-on procedure to high pressure freezing and other cryo fixation methods. It is the process of dehydration of the frozen specimen by an organic solvent at low temperature in the presence of a secondary fixative. The Leica EM AFS2 freeze substitution and low temperature embedding system features a remote control that makes it easy to access your system at any time from any location, and be in control of your experiment and laboratory.