Decode 3D biology in real time*

THUNDER Imager Tissue

The THUNDER Imager Tissue allows real-time fluorescence imaging of 3D tissue sections typically used in neuroscience and histology research. Acquire rich, detailed images of thick tissues free of haze from out-of-focus blur.

Even fine structures deep in tissues can be resolved thanks to Computational Clearing, an innovative Leica technology. Image detailed morphological structures like axons and dendrites of neurons in a brain slice. The high image quality, even with thick tissue sections, is combined with the well-known speed, fluorescence efficiency, and ease of use of widefield microscopes.

Gain these advantages using a THUNDER Imager Tissue for your research:

  • Rapidly acquire blur-free images showing finest details of the morphology, even deep within thick sections
  • Get fast overviews of whole tissue sections
  • Image and analyze challenging tissue sections with an easy workflow

*in accordance with ISO/IEC 2382:2015

Details fully resolved even in thick specimens

THUNDER Imager Tissue enables the meaningful exploration of thick tissue specimens with all the advantages of widefield microscopy. See the details of structures even deep in your specimen. It does not matter if you acquire single sections or 3D z-stacks.

The investigation of neuronal networks is a perfect example of what is possible. With Computational Clearing, a Leica technique, you remove the out-of-focus blur in real time and make the fine structure of specific brain areas visible.

Now you can follow the dynamics of reorganization within a neuronal network or the rebuilding and establishment of new synapses. In other words you can decode 3D biology in real time.

Slider: YFP mouse brain slices stained with GFAP-A647. Imaged with a THUNDER Imager Tissue. Courtesy Dr. Hong Xu, University of Pennsylvania, Philadelphia (USA).

Widefield
THUNDER Imager Tissue

Drosophila third instar larval fillet labeled with AlexaFluor™647 for post-synaptic sites, AlexaFluor™555 conjugated to phalloidin, and AlexaFluor™488 labeling a subset of motor neurons.

Image your whole specimen in a breathtakingly short time

Image rapidly the whole specimen with the THUNDER Imager Tissue. You can acquire outstanding images of thick specimens in one shot showing the finest cellular structures.

Now it is easy to collect clear, detailed images of larger tissue sections.

Combine THUNDER Imager Tissue with the LAS X Navigator software for a blur-free overview of your entire tissue specimen.

Quickly navigate your specimen and find regions of interest.

Video: Sample courtesy Dr. Amicia Elliott, NIH/NIMH, Bethesda, MD (USA).

Resolve fine details in challenging specimens

THUNDER Imager Tissue delivers instantly cleared-up fluorescent images with fine details of your multi-color tissue sections. Just turn the THUNDER Imager on and get started! The unique Computational Clearing uses optimal parameters to produce expert-level results. It does it automatically with no need for calibration or user interaction.

Achieve optimal fluorescence and contrast settings instantly via the patented Leica fluorescence intensity (FIM) and contrast managers. Choose from a range of objectives, optimized for specific applications, to ensure outstanding results even for challenging specimens.

Video: Sample courtesy Dipl. Biol. Thomas Frase, University of Rostock, Allgemeine & Spezielle Zoologie, Institut for Biosciences, Rostock (Germany).

Cyclops sp. Nuclei (green), acetylated -tubulin (red), Serotonin (cyan). Image stack of a total volume of 332 x 332 x 84 µm³ with three colors and 305 z planes per color. Objective used was HC PL FLUOTAR 40x/1.30 OIL.

THUNDER Imager 3D Tissue

Two Options for THUNDER Imager Tissue

Choose the configuration which best meets your requirements:

  • THUNDER Imager 3D Tissue is a fully automated tissue imaging system for recording multi-color 3D images. Capture multiple images in the z-direction and visualize them in the 3D viewer. Achieve excellent z-stack imaging due to a precise motorized focus drive. Decode 3D structures in real time.

  • For brilliant imaging of single planes and fast overviews of your tissue the THUNDER Imager Tissue is also available in a cost-effective configuration without a motorized focus drive.

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