High Resolution Array Tomography with Automated Serial Sectioning

Faster 3D Imaging of Cellular and Protein Structures


The optimization of high resolution, 3-dimensional (3D), sub-cellular structure analysis with array tomography using an automated serial sectioning solution, achieving a high section density on the carrier substrate, is described in this report.

Array tomography

Array tomography (AT) is a 3D image reconstruction technique for biological specimens. It involves the imaging of ordered arrays of ultrathin serial sections with scanning electron microscopy (SEM) and enables high resolution, quantitative analysis of biological structures. AT offers higher spatial resolution than conventional confocal microscopy. The method can reveal additional information of cellular and protein structures to clarify poorly understood or unresolved features.

Optimized workflow with automated ultramicrotome

The workflow for array tomography is better optimized when using an automated ultramicrotome that can achieve uniform, ultrathin serial sections for orderly array image acquisition with the SEM. An example is the ARTOS 3D solution from Leica Microsystems. It preserves a high section quality by eliminating artifacts that may occur during the section ribbon collection process from manual handling typically done with conventional ultramicrotomes.

Get Free Report

Please fill in the form below and you will receive the full article by email.

Get the free report

Get the free report

By clicking on the SUBMIT button, I confirm that I have reviewed and agree with Leica Microsystems GmbH Terms of Use and their Privacy Policy. I also understand my privacy choices as they pertain to my personal data, as detailed in the aforementioned Privacy Policy under ‘’Your Privacy Choices’’.

Related Articles

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting? Show local contacts

Scroll to top