How to Improve Live Cell Imaging with Coral Life

HeLa Kyoto cells (HKF1, H2B-mCherry, alpha Tubulin, mEGFP). Left image: Maximum projection of a z-stack prior to ICC and LVCC. Right image: Maximum projection of a mosaic z-stack after ICC and LVCC. How_to_improve_live_cell_imaging_with_Leica_Nano_Workflow_teaser.jpg

For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on the advantages of working with a widefield system and which obstacles need to be overcome when using sapphire as a cell-culture substrate.

Key Advantages

  • Fast cellular events can be captured for subsequent examination by Cryo Electron Microscopy.
  • Benefit from the Leica innovative technology Computational Clearing.
  • Efficient removal of out-of-focus blur.
  • Accurate targeting of proteins or cellular structures of interest for downstream ultrastructural analysis.

Register for Download

By clicking on the SUBMIT button, I confirm that I have reviewed and agree with Leica Microsystems GmbH Terms of Use and their Privacy Policy. I also understand my privacy choices as they pertain to my personal data, as detailed in the aforementioned Privacy Policy under ‘’Your Privacy Choices’’.

Related Articles

Related Pages

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting?