How to Keep Your Samples Under Physiological Conditions

Koenig, Julia; Rantanen, Krista

July 13, 2021 Whitepaper

How to Keep Your Samples Under Physiological Conditions

The Leica Nano Workflow

Leica Microsystems’ Nano workflow combines dynamic data with the best possible sample fixation by high pressure freezing. However, good sample preservation won’t help if your cells are stressed by temperature drops or a shortage of oxygen (O₂), carbon dioxide (CO₂), or nutrients. These factors will influence a variety of biological processes down to their underlying ultrastructural basis and impair your analysis.

Therefore, Leica Microsystems worked together with Baker Ruskinn to develop a way to keep your samples under physiological conditions from the moment of cell seeding until high-pressure freezing.

With this solution, samples can be grown, selected, and imaged without any time pressure. In this article, it is described why controlling O₂ is especially important and how it is addressed in the Leica Nano workflow together with Baker Ruskinn.

Authors

¹Leica Microsystems

Topics & Tags

EM Sample Preparation

Key Learnings

Why is the constant control of oxygen important?
OxyGenie by Baker Ruskinn
Adapted for the Leica Nano workflow
Cell viability and cytotoxicity in SampLink chambers

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How to Keep Your Samples Under Physiological Conditions