Fixation and Dehydration
3% Glutaraldehyde in PBS, pH 7.3 at 4°C | 16 h |
Distilled water | 3x10 min. |
1% aqueous OsO4, at 4°C | 16 h |
Distilled water | 3x10 min. |
Ethanol series: 30%, 50%, 70%, 80%, 90%, 96%, 100% at 25°C | 1x10 min. |
Acetone series: 30%, 50%, 100% | 1x10 min. |
Leica EM CPD300 auto program
![](/fileadmin/_processed_/f/1/csm_Bacteria_Protocol_List_1_8e77441ae1.jpg)
![](/fileadmin/_processed_/c/2/csm_Bacteria_Protocol_List_2_f1e1234b2f.jpg)
Coating
Mount the dried samples on stubs containing carbon adhesives.
Platinum / Palladium coating: 6 nm