Contact Us

Bacteria Protocol - Critical Point Drying of E. coli for SEM

Application Note for Leica EM CPD300 - Life Science Research

Bacteria_Protocol_part.jpg

Introduction
Species: Escherichia coli
Critical point drying of E. coli with subsequent platinum / palladium coating and SEM analysis.

Procedure
Sample Holder:
Sample was inserted into a filter disc (Pore size: 16 - 40 μm) and placed into the filter discs and porous pots holder.

Cultivation
Cultivate fungi and bacteria on agar containing growth medium for 3 days. Selected parts of the colonies of bacteria.

Fixation and Dehydration

3% Glutaraldehyde in PBS, pH 7.3 at 4°C16 h
Distilled water3x10 min.
1% aqueous OsO4, at 4°C16 h
Distilled water3x10 min.
Ethanol series: 30%, 50%, 70%, 80%, 90%, 96%, 100% at 25°C1x10 min.
Acetone series: 30%, 50%, 100%1x10 min.

Leica EM CPD300 auto program

Coating
Mount the dried samples on stubs containing carbon adhesives.
Platinum / Palladium coating: 6 nm

Fig.1: E. coli
Fig.2: E. coli

Download PDF

Related Articles

Related Pages

Background image

Interested to know more?
Talk to our experts.

Price

I need a configuration or price info.

Service & Support

I need technical support, service contract or software info.

Demo

I need an on site or remote demo.

Application

I need assistance/training to operate my system.

Do you prefer personal consulting? Show local contacts

Scroll to top