The sections were imaged using a Tecnai Polara 300KeV (FEI, The Netherlands) microscope fitted with a 4K Gatan
Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) of Yeast
Application note for Leica EM HPM100 - Life Science Research

Protocol
The sections are of yeast frozen with a Leica HPM100 High Pressure Freezer in the copper tube system, the cell paste was mixed with a pH 6.5 MES/dextran buffer so that a final MES concentration of 50mM and a dextran concentration of 20% was achieved.
The samples were sectioned on a Leica EM UC7/ EM FC7 Ultramicrotome with micromanipulator at -140°C and the section thickness was set to 50nm. The sections were attached to Agar lacey grids.