The sections were imaged using a Tecnai Polara 300KeV (FEI, The Netherlands) microscope fitted with a 4K Gatan CCD camera. The magnification for the sections was 23K, with a defocus of -6um for the tomogram and -8um for the projection image, and the diffraction was done with a camera length of 930mm. The image in panel A is an average of the central 10 slices of a reconstruction done with the IMOD package (Kremer et. al., 1996), image processing software from a tomogram collected using the FEI software.
Left – optical slice from a tomographic reconstruction
Upper right – micrograph of a vitrified yeast cell
Lower right – diffraction pattern image
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