In this episode of MicaCam, our special guest George Galea from EMBL Heidelberg will look at HeLa Kyoto cells treated with various chemotherapeutic agents to investigate their effect on the Golgi complex and the cell nucleus organization and positioning. The Golgi complex is an important homeostasis hub, where a multitude of signaling pathways and essential cellular processes intersect. The structure and the organization of the organelle is critical for its function and the overall health of the cell.
Recording
Recording will be available soon.
Key Learnings
- How the Golgi morphology in a cell population differs in response to various types of cell stress
- How to classify the different Golgi organizational conformations using widefield and confocal microscopy
- How to set up a screening pipeline to study the architecture of the organelle
Speakers
Dr. George Galea
Researcher – Pepperkok Team, EMBL Heidelberg
George is a researcher working in Pepperkok team at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany. His research focuses on understanding how the Golgi complex interacts with other organelles to coordinate various cellular processes in response to physiologically relevant cues such as DNA damage events. To better understand these processes, he applies multi-omic approaches including high-throughput imaging to dissect the Golgi structure organization and DNA repair mechanics.
Dr. Lynne Turnbull, Senior Application Manager – Leica Microsystems
Lynne is a Senior Application Manager at Leica Microsystems. She received her PhD in Sydney Australia in cardiac biophysics and undertook postdoctoral training in San Francisco and Melbourne. Upon moving to the University of Technology Sydney, Lynne established and managed the Microbial Imaging Facility. Lynne joined Leica Microsystems in 2021 as a Senior Application Manager and is based at the EMBL IC in Heidelberg.
