Human Blood Cells Protocol

Application Note for Leica EM CPD300 - Life Science Research


Species: Human (Homo sapiens)
Critical point drying of human blood with subsequent platinum / palladium coating and SEM analysis

Sample Holder:
Samples were inserted into the 12 mm cover slip holder

Place 12 mm dia cover slip poly-L-lysine coated in a 12-wells cell culture plate. Add 1 ml 0.85% NaCl in each well to submerge each cover slip.
Pipette gently 50 μl blood on each glass cover slip leave for 5 min at 25°C.
Add 200 μl 0.2 M CaCl2 on top of the blood cells to activate the platelets and leave for 10 min.

Fixation and Dehydration:
Add gently 1 ml of 4% Paraformaldehyde, 0.4% Glutaraldehyde in 0.2 M Sodium Cacodylate Buffer, pH 7.2, on top of the blood cells and leave at least for 10 min. at RT.

Distilled Water3x10 min.
1% aqueous OsO4, 4°C16 h
Distilled water3x10 min.
Ethanol series: 30%, 50%, 70%, 80%, 90%, 96%, 100%1x10 min.
Acetone series: 30%, 50%, 100%1x10 min.

Leica EM CPD300 auto Program:

Mount the dried samples on stubs containing carbon adhesives
Platinum / Palladium coating: 6 nm

Download PDF

Related Articles

Related Pages

Interested to know more?

Talk to our experts. We are happy to answer all your questions and concerns.

Contact Us

Do you prefer personal consulting? Show local contacts

Scroll to top