Ciencias de la vida
Ciencias de la vida
Este es el lugar para ampliar sus conocimientos, capacidades de investigación y aplicaciones prácticas de la microscopía en diversos campos científicos. Aprenda a conseguir una visualización precisa, interpretación de imágenes y avances en la investigación. Encuentre información detallada sobre microscopía avanzada, técnicas de obtención de imágenes, preparación de muestras y análisis de imágenes. Los temas tratados incluyen la biología celular, la neurociencia y la investigación del cáncer, con especial atención a las aplicaciones e innovaciones de vanguardia.
What’s the Best Organoid Imaging Approach for Early Drug Discovery?
Organoids and other complex in vitro models (CIVMs) are becoming increasingly important in early drug discovery and translational research, driven by the need for more predictive, human-relevant data…
Waffle Method Workflow: From HPF to Cryo-ET Lamellae
Waffle freezing provides an HPF-based route to cryo-ET sample preparation. This workflow guide follows the process from grid and carrier assembly to vitrification, cryo-FIB milling, lamella…
Fast, High-Contrast Widefield Imaging of Optically Challenging Samples
Live‑cell imaging of large, complex biological samples often requires large fields of view, sub-cellular resolution, high-sensitivity, and fast acquisition – all while maintaining low illumination…
Spatial Proteomics Workflow in Blood Cancer (MPNs)
Megakaryocytes play a central role in the biology of myeloproliferative neoplasms (MPNs), yet their in vivo proteomic characterization remains a major challenge due to low abundance and disrupted…
Multiscale Imaging of Organoids: High Content to Light Sheet
Learn multiscale organoid imaging: fixed high content phenotyping, gentle dual view light sheet, and reproducible pipelines that turn 3D data into insights.
What is FRET with FLIM or as it is usually known FLIM-FRET?
Förster resonance energy transfer (FRET) is a well-established fluorescence-based technique which is used to study molecular interactions. It is useful for the analysis of protein-DNA and…
Ratiometric Imaging and Analysis of Ion Concentration in Cells
Many cellular functions depend on the dynamic balance of ions, electric potentials, and pH between the cytosol and surrounding extracellular space. Changes in these values affect cellular function.…
High-Pressure Freezing Protocols for Ultrastructural 3D EM
High pressure freezing (HPF) can help preserve hydrated cells and tissues close to their biological state at the moment of immobilization, supporting more reliable ultrastructural interpretation than…
Ultramicrotome UC Enuity in Practice: Stable 15 nm Sections at ZFE
After using the UCT and UC6 ultramicrotomes, Claudia Mayrhofer calls UC Enuity a leap in stability—so robust that vibrations and temperature shifts don’t spoil sections, even with multiple users. Auto…
