Multiscale Imaging of Organoids: High Content to Light Sheet

This webinar explains multiscale imaging workflows for organoid imaging that combine fixed-tissue high-content imaging with specialized long-term live imaging using light sheet microscopy to obtain the “necessary statistical and causal information” for understanding 3D cultured systems.

Organoids are presented as an accessible in vitro model for intestinal tissue regeneration and homeostasis that closely mimics in vivo dynamics, while also introducing key imaging challenges: high variability in morphology and cell-type composition, phototoxicity sensitivity in live imaging, and culture timelines spanning days to weeks (and longer depending on organoid type).

First, Dr. Gustavo Quintas Glasner de Medeiros walks through high-throughput fixed time courses: dissociate to single cells, plate multi-well plates, fix plates at staged timepoints and acquire 3D datasets at scale on a high-content imager (spinning-disc confocal is discussed). He explains how this approach supports phenotypic landscape profiling from large numbers of organoids, and how multiplexed immunostaining can add functional readouts by iteratively eluting and re-staining for additional markers.

To conclude the webinar, Dr. Andrea Boni presents the key features of the Viventis Deep Dual View Light Sheet Microscope and introduces its new imaging option for cleared samples. He explains how the Viventis Deep can be used for 3D live imaging and for cleared sample imagaing simply by exchanging the objective block.

His examples shown include cleared organoid-scale samples up to larger mouse specimens, such as a cleared gastroloid imaged in three colors at single-cell resolution and a tiled mouse salivary gland explant dataset.