Optical properties

  • λ abs = 542 nm
  • ε max = 1.2×105 M -1 cm -1
  • λ fl = 562 nm
  • n fl = 93 %
  • τ fl = 3.7 ns
  • CF 260 = ε 260 /ε max = 0.18
  • CF 280 = ε 280 / ε max = 0.08
    • ATTO 542 is a new fluorescent label related to ATTO 532.

      The new dye is very hydrophilic and shows excellent water solubility. Characteristic features of the label are strong absorption and high photo-stability. The dye shows a very high fluorescence quantum yield even after coupling to a biomolecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy (PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.

      After coupling to a substrate the dye carries a net electrical charge of - 3. The fluorescence is excited most efficiently in the range 520 - 555 nm.

ATTO 542

ATTO 542 is a new fluorescent label related to ATTO 532.

The new dye is very hydrophilic and shows excellent water solubility. Characteristic features of the label are strong absorption and high photo-stability. The dye shows a very high fluorescence quantum yield even after coupling to a biomolecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy (PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.

After coupling to a substrate the dye carries a net electrical charge of - 3. The fluorescence is excited most efficiently in the range 520 - 555 nm.

Fields of Application

Life Science Research

Leica Microsystems’ life science research microscopes support the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and…
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