Optical properties

  • λ abs = 554 nm
  • ε max = 1.2×105 M -1 cm -1
  • λ fl = 576 nm
  • n fl = 80 %
  • τ fl = 3.6 ns
  • CF 260 = ε 260max = 0.23
  • CF 280 = ε 280 / ε max = 0.10

ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability.

The dye is moderately hydrophilic. ATTO 550 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. As supplied ATTO 550 consists of three isomers with practically identical absorption and fluorescence.

The fluorescence is excited most efficiently in the range 540 - 565 nm.

ATTO 550

ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability.

The dye is moderately hydrophilic. ATTO 550 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. As supplied ATTO 550 consists of three isomers with practically identical absorption and fluorescence.

The fluorescence is excited most efficiently in the range 540 - 565 nm.

Fields of Application

Life Science Research

Leica Microsystems’ life science research microscopes support the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and…
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