Optical properties

  • λ abs = 564 nm
  • ε max = 1.2×105 M -1 cm -1
  • λ fl = 590 nm
  • n fl = 90 %
  • τ fl = 4.0 ns
  • CF 260 = ε 260max = 0.27
  • CF 280 = ε 280 / ε max = 0.12

ATTO 565 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 565 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.

The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 545 - 575 nm. As supplied ATTO 565 consists of a mixture of two isomers with practically identical absorption and fluorescence. However, on request the dye can also be supplied as single isomer.

ATTO 565

ATTO 565 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 565 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.

The dye is moderately hydrophilic. The fluorescence is excited most efficiently in the range 545 - 575 nm. As supplied ATTO 565 consists of a mixture of two isomers with practically identical absorption and fluorescence. However, on request the dye can also be supplied as single isomer.

Fields of Application

Life Science Research

Leica Microsystems’ life science research microscopes support the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and…
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