ATTO 576H NHS-Ester Hydrophilic Fluorescent Label 5 mg
Product No. AD576H35
ATTO 576H is a fluorescent marker out of the class of rhodamines. As a hydrophilic alternative to ATTO Rho12, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding.
"ATTO-TEC is a wholly owned subsidiary of Leica Microsystems"
Optical properties
- λ abs = 576 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 598 nm
- n fl = 90 %
- τ fl = 4.0 ns
- CF 260 = ε 260 /ε max = 0.26
- CF 280 = ε 280 / ε max = 0.10
ATTO 576H is a fluorescent marker from the class of rhodamines. As ahydrophilic alternative to ATTO Rho12, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding. ATTO 576H possesses strong absorption, a very high fluorescence quantum yield, and high thermal and photochemical stability. The dye is therefore ideal for use in single-molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 576H is an anionic dye. After coupling to a substrate, it is singly negatively charged. ATTO 576H is a mixture of three isomers with virtually identical absorption and fluorescence properties. Efficient fluorescence excitation occurs in the 550–585 nm range.
ATTO 576H
ATTO 576H is a fluorescent marker out of the class of rhodamines. As a hydrophilic alternative to ATTO Rho12, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding.
Optical properties
- λ abs = 576 nm
- ε max = 1.2×105 M -1 cm -1
- λ fl = 598 nm
- n fl = 90%
- τ fl = 4.0 ns
- CF 260 = ε 260 /ε max = 0.26
- CF 280 = ε 280 / ε max = 0.10
ATTO 576H is a fluorescent marker out of the class of rhodamines. As a hydrophilic alternative to ATTO Rho12, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding. ATTO 576H has a strong absorption, very high fluorescence quantum yield and a high thermal and photochemical stability. The dye is therefore perfectly suited for use in single molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 576H is an anionic dye. After coupling to a substrate, it carries a net electrical charge of -1. ATTO 576H is a mixture of three isomers with practically identical absorption and fluorescence properties. Efficient excitation of fluorescence occurs in the 550 - 585 nm range.
ATTO-TEC is a wholly owned subsidiary of Leica Microsystems
