ATTO 542

ATTO 542 is a new fluorescent label related to ATTO 532.

The new dye is very hydrophilic and shows excellent water solubility. Characteristic features of the label are strong absorption and high photo-stability. The dye shows a very high fluorescence quantum yield even after coupling to a biomolecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy (PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.

After coupling to a substrate the dye carries a net electrical charge of - 3. The fluorescence is excited most efficiently in the range 520 - 555 nm.

View products

Popular Configurations

Effective April 7, 2025, Leica Microsystems transactions will apply an adjustment tariff charge due to new U.S. tariffs on all applicable products.

Modification

Unit of measure

ATTO 542 Biotin Fluorescent Label 1 mg

Product No. AD-542-71

ATTO 542 is a new fluorescent label related to ATTO 532

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

View product details+

ATTO 542 Biotin Fluorescent Label 5 mg

Product No. AD-542-75

ATTO 542 is a new fluorescent label related to ATTO 532

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

View product details+

ATTO 542 Carboxy Fluorescent Label 1 mg

Product No. AD-542-21

ATTO 542 is a new fluorescent label related to ATTO 532

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

View product details+

ATTO 542 Carboxy Fluorescent Label 5 mg

Product No. AD-542-25

ATTO 542 is a new fluorescent label related to ATTO 532

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

View product details+

ATTO 542 DPPE Labeled Phospholipid 1 mg

Product No. AD-542-151

ATTO-Dye Labeled Phospholipids

In recent years the investigation of biological membranes, e.g. intracellular membranes of live cells, plasma membranes etc., has become a major subject of interest. As a result there is a growing demand for fluorescent dye-labeled lipids, in particular glycero-phospholipids, to be incorporated in biological membranes. ATTO-TEC offers a variety of glycero-phospholipids, carrying one or two fatty acid chains (lipophilic groups) and a phosphate ester residue (hydrophilic group).

ATTO-dye fluorescent phospholipids are labeled at the hydrophilic head group. After incorporation in, e.g., a plasma membrane (phospholipid bilayer) the fluorophore is located at the water/lipid interface of the membrane.

"ATTO-TEC is a wholly owned subsidiary of Leica Microsystems"