Regulators of Actin Cytoskeletal Regulation and Cell Migration in Human NK Cells
Dr. Mace will describe new advances in our understanding of the regulation of human NK cell actin cytoskeletal remodeling in cell migration and immune synapse formation derived from confocal and…Read articleHuman NK Cell Development Requires CD56-mediated Motility and Formation of the Developmental Synapse
While distinct stages of natural killer (NK) cell development have been defined, the molecular interactions that shape human NK cell maturation are poorly understood. Here we define intercellular…Read articleVisualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse…Read articleVisualization of the Natural Killer Cell Immune Synapse by Super-Resolution Nanoscopy
Natural killer (NK) cells are innate immune effectors that recognize and kill virally infected and tumorigenic cells. Central to their effector function is the formation of an immunological synapse…Read articleDual Channel STED Nanoscopy of Lytic Granules on Actin Filaments in Natural Killer Cells
Natural killer (NK) cells are innate immune effectors that eliminate diseased and tumorigenic targets through the directed secretion of specialized secretory lysosomes, termed lytic granules. This…Read article