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Live 3D FLIM on patient-derived organoid

Monitoring cancer-derived increased of signal transduction activity in live organoids with an ERK FRET-FLIM biosensor14 (right). Corresponding fluorescence intensity image with no functional information. Functional information showing FRET efficiency of the ERK biosensor. Higher FRET efficiency indicates higher ERK activity. Color bar, 2–12% FRET efficiency. Scale bar, 20 μm.

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Courtesy of Bas Ponsioen UMC Utrecht, Kees Jalink, Bran van den Broek, Netherlands Cancer Institute

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