Topics covered
The following topics will be addressed during lectures, tutorials, and practical workshops:
- Sample preparation for STED microscopy, including choice of fluorophores and refractive index matching
- Technical implementations: pulsed-STED, gated-STED, and time-resolved STED (tauSTED)
- Multi-color 2D and 3D STED microscopy
- Image acquisition strategies for live-cell STED
- STED multiplexing via lifetime-based fluorophore separation
- Clearing for large volume STED imaging and adaptive motCORR (motorized correction) objectives
- Expansion-microscopy STED
- Deconvolution of time-resolved STED data
- Image analysis
Intended outcomes
At the end of the course, the participants will be able to design experiments using STED microscopy, prepare high-quality microscopy samples, and acquire state-of-the-art nanoscopy images.
Date: 13 - 18 July, 2026
Location: on site at the EMBL Imaging Center
Hosted by: Leica Microsystems and EMBL
More Info: https://www.embl.org/about/info/course-and-conference-office/events/let26-01/
What past participants say about the course:
The EMBL super-resolution microscopy course was exactly tailored to my needs and will allow me to get my own experiments running independently. Also, contacts I made will be very useful in the future!” – Paul Markus Müller, Freie Universität Berlin, Germany
Very well organized and conceived course. Everything is covered here including the fundamentals, the practical sessions, creative application, innovative development of STED technology. If you are interested in nanoscopy, I would recommend you participate in this course.” – Shao-Chun ‘Peggy’ Hsu, National Taiwan University, Taiwan
