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Murine esophageal organoids (DAPI, Integrin26-AF 488, SOX2-AF568) imaged with the THUNDER Imager 3D Cell Culture. Courtesy of Dr. F.T. Arroso Martins, Tamere University, Finland.

How to Get Deeper Insights into your Organoid and Spheroid Models

In this eBook, learn about key considerations for imaging 3D cultures, such as organoids and spheroids, and discover microscopy solutions to shed new insights into dynamic processes in 3D real-time
Fluorescence microscopy image of liver tissue where DNA in the nuclei are stained with Feulgen-pararosanilin and visualized with transmitted green light.

Epi-Illumination Fluorescence and Reflection-Contrast Microscopy

This article discusses the development of epi-illumination and reflection contrast for fluorescence microscopy concerning life-science applications. Much was done by the Ploem research group…
Molecular structure of the green fluorescent protein (GFP)

Introduction to Fluorescent Proteins

Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.
Branched organoid growing in collagen where the Nuclei are labeled blue. To detect the mechanosignaling process, the YAP1 is labeled green.

Examining Developmental Processes In Cancer Organoids

Interview: Prof. Bausch and Dr. Pastucha, Technical University of Munich, discuss using microscopy to study development of organoids, stem cells, and other relevant disease models for biomedical…
Masson-Goldner staining of a hedgehog brain slice.

How to Image Histological and Fluorescent Samples with One System

VIDEO ON DEMAND - How to image histological and fluorescent samples with one system. FluoSync, the new technology embedded into Mica enables the imaging of both histological staining and fluorescence…

How to Radically Simplify Workflows in Your Imaging Facility

VIDEO ON DEMAND - How to radically simplify imaging workflows and generate meaningful results with less time and effort using a highly automated microscope that unites widefield and confocal imaging.

FluoSync - a Fast & Gentle Method for Unmixing Multicolour Images

In this white paper, we focus on a fast and reliable method for obtaining high-quality multiplex images in fluorescence microscopy. FluoSync combines an existing method for hybrid unmixing with…

Harnessing Microfluidics to Maintain Cell Health During Live-Cell Imaging

VIDEO ON DEMAND - In this episode of MicaCam, we will use microfluidics to explore the effect of shear stress on cell morphology, examine the effect of nutrient replenishment on cellular growth during…
Living HeLa cells stained with WGA-488 (yellow), SPY-Actin (cyan), and SiR-Tubulin (magenta). Instant Computational Clearing (ICC) was applied.

How to Perform Dynamic Multicolor Time-Lapse Imaging

Live-cell imaging sheds light on diverse cellular events. As many of these events have fast dynamics, the microscope imaging system must be fast enough to record every detail. One major advantage of…
Two-color caspase assay with tile scan. U2OS cells were treated with the nuclear marker DRAQ5 (magenta) and CellEvent™ (yellow).

Following Multiple Events during Staurosporine Apoptosis

VIDEO ON DEMAND - In this episode of MicaCam, we show how adding additional markers to an apoptosis kit can markedly increase the amount of information a researcher can obtain from the same…
Untreated Hela Kyoto cells stained to show the nucleus (Hoechst, blue), the cis-golgi matrix protein GM130 (AF488, green), and the trans-golgi network membrane protein TGN46 (AF647, red).

Golgi Organizational Changes in Response to Cell Stress

VIDEO ON DEMAND - In this episode of MicaCam, our special guest George Galea from EMBL Heidelberg will look at HeLa Kyoto cells treated with various chemotherapeutic agents to investigate their effect…
Protist Paramecium (Paramecium tetraurelia) stained to show the nucleus

AI-Enabled Spatial Analysis of Complex 3D Datasets

VIDEO ON DEMAND - This edition of MicaCam offers practical advice on the extraction of publication grade insights from microscopy images. Our special guest Luciano Lucas (Leica Microsystems) will…
3D Reconstruction of brain slide image_Mica

3D Tissue Imaging: From Fast Overview To High Resolution With One Click

3D Tissue imaging is a widespread discipline in the life sciences. Researchers use it to reveal detailed information of tissue composition and integrity, to make conclusions from experimental…
MDCK cysts on day 9

How To Perform Fast & Stable Multicolor Live-Cell Imaging

With the help of live-cell imaging researchers gain insights into dynamic processes of living cells up to whole organisms. This includes intracellular as well as intercellular activities. Protein or…
Zebrafish heart showing the ventricle with an injury in the lower area

Cardiomyocyte Proliferation Upon Heart Injury in Zebrafish

VIDEO ON DEMAND - This edition of MicaCam focuses on the study of the zebrafish (Danio rerio) whose heart cells can fully regenerate after injury.

How Does The Cytoskeleton Transport Molecules?

VIDEO ON DEMAND - See how 3D cysts derived from MDCK cells help scientists understand how proteins are transported and recycled in tissues and the role of the cytoskeleton in this transport.
Developing zebrafish (Danio rerio) embryo, from sphere stage to somite stages.

Studying Early Phase Development of Zebrafish Embryos

VIDEO ON DEMAND - This second edition of MicaCam focuses on combining widefield and confocal imaging to study the early-stage development of zebrafish embryos (Danio rerio), from oocyte to…
Two-color caspase assay with tile scan

Multi-Color Caspase 3/7 Assays with Mica

Caspases are involved in apoptosis and can be utilized to determine if cells are undergoing this programmed cell death pathway in so-called caspase assays. These assays can be run by e.g. flow…
U2OS cells stained with Hoechst for nuclei (blue), MitoTracker green (Mitochondria structure, green) and TMRE (active mitochondria, magenta) and SiR for tubulin (red). Simultaneous acquisition of four channel large area overview using Spiral scan feature using the 10x/1.20 CS2 Water MotCORR objective.

How To Get Multi Label Experiment Data With Full Spatiotemporal Correlation

VIDEO ON DEMAND - The first edition of MicaCam focuses on the special challenges of live cell experiments. Our hosts Lynne Turnbull and Oliver Schlicker use the example of studying the mitochondrial…
U2OS cells labelled with SiR Actin, TMRE, CellEvent™, and DAPI; 13-hour time-lapse imaging; apoptosis-inducer staurosporine

Simplifying Complex Fluorescence Multiwell Plate Assays

Apoptosis, or programmed cell death, occurs during organism embryo development to eliminate unwanted cells and during healing in adults to rid the body of damaged cells and help prevent cancer.…
Formation of 3D spheroids; Time lapse acquisition over 72 hours

Efficient Long-term Time-lapse Microscopy

When doing time-lapse microscopy experiments with spheroids, there are certain challenges which can arise. As the experiments can last for several days, prolonged sample survival must be achieved…

How to Prepare your Specimen for Immunofluorescence Microscopy

Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM,…

Live-Cell Imaging Techniques

The understanding of complex and/or fast cellular dynamics is an important step for exploring biological processes. Therefore, today’s life science research is increasingly focused on dynamic…

Fluorescent Dyes

A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP –…

Applying AI and Machine Learning in Microscopy and Image Analysis

Prof. Emma Lundberg is a professor in cell biology proteomics at KTH Royal Institute of Technology, Sweden. She is also the director of the Cell Atlas, an integral part of the Swedish-based Human…

Using Machine Learning in Microscopy Image Analysis

Recent exciting advances in microscopy technologies have led to exponential growth in quality and quantity of image data captured in biomedical research. However, analyzing large and increasingly…

The AI-Powered Pixel Classifier

Achieving reproducible results manually requires expertise and is tedious work. But now there is a way to overcome these challenges by speeding up this analysis to extract the real value of the image…
Image: Adult rat brain. Neurons (Alexa Fluor488, green), Astrocytes (GFAP, red), Nuclei (DAPI, blue). Image courtesy of Prof. En Xu, Institute of Neurosciences and Department of Neurology of the Second Affiliated Hospital of Guangzhou Medical University, China.

Multicolor Microscopy: The Importance of Multiplexing

The term multiplexing refers to the use of multiple fluorescent dyes to examine various elements within a sample. Multiplexing allows related components and processes to be observed in parallel,…

Considerations for Multiplex Live Cell Imaging

Simultaneous multicolor imaging for successful experiments: Live-cell imaging experiments are key to understand dynamic processes. They allow us to visually record cells in their living state, without…

A New Method for Convenient and Efficient Multicolor Imaging

The technique combining hyperspectral unmixing and phasor analysis was developed to simplify the process of getting images from a sample labeled with multiple fluorophores. This aggregate method…
Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imagerwith a 2x 0.15 NA objective at 3.4x zoomwas used to produce this 425 μm Z-stack (26 positions), which is presented here as an Extended Depth of Field(EDoF)projection.

Neuroscience Images

Neuroscience commonly uses microscopy to study the nervous system’s function and understand neurodegenerative diseases.

Introduction to Widefield Microscopy

This article gives an introduction to widefield microscopy, one of the most basic and commonly used microscopy techniques. It also shows the basic differences between widefield and confocal…

Nobel Prize 2013 in Physiology or Medicine for Discoveries of the Machinery Regulating Vesicle Traffic

On October 7th 2013, The Nobel Assembly at Karolinska Institutet has decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to James E. Rothman, Randy W. Schekman and Thomas C. Südhof…
John B. Gurdon

Nobel Prize 2012 in Physiology or Medicine for Stem Cell Research

The Nobel Prize recognizes two scientists who discovered that mature, specialised cells can be reprogrammed to become immature cells capable of developing into all tissues of the body. Their findings…
Primary leaves of cowpea (Vigna unguiculata "California Blackeye") inoculated with cowpea mosaic virus (CPMV) containing the GFP-gene inserted between the movement protein (MP) and the capsid proteins (CPs) in the viral RNA 2

Introduction to Live-Cell Imaging

The understanding of complex and fast cellular dynamics is an important step to get insight into biological processes. Therefore, today’s life science research more and more demands studying…
Fluorescence microscope image of a life-science specimen

An Introduction to Fluorescence

This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.

Campos de aplicación

Microscopía de fluorescencia

La fluorescencia es uno de los fenómenos físicos más utilizados en la microscopía biológica y analítica, principalmente por su alta sensibilidad y alta especificidad. Averigüe cómo los microscopios de…

Biología celular

Descubra cómo puede extraer el máximo rendimiento a sus investigaciones en materia de biología celular y amplíe sus descubrimientos con las soluciones de generación de imágenes de Leica Microsystems.

Investigación del cáncer

El cáncer es una enfermedad compleja y heterogénea causada por células con una regulación deficiente del crecimiento. Los cambios genéticos y epigenéticos en una célula o en un grupo de ellas alteran…

Imagen de célula viva

Con un cambio de perspectiva desde componentes individuales del microscopio a una solución de captura y procesamiento de imágenes de células vivas totalmente operativa, Leica Microsystems integra…

Organoides y cultivos celulares en 3D

Uno de los avances recientes más emocionantes en la investigación de las ciencias biológicas es el desarrollo de sistemas de cultivo celular en 3D, como organoides, esferoides y modelos de órganos en…

Organismos modelo en la investigación

Un organismo modelo es una especia empleada por los investigadores para estudiar procesos biológicos específicos. Sus características genéticas son similares a las de los humanos y se emplean de forma…


¿Trabaja para comprender mejor las enfermedades neurodegenerativas o la función del sistema nervioso? Vea cómo puede conseguir descubrimientos con las soluciones de imágenes de Leica Microsystems.
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