Ciencias de la vida

Ciencias de la vida

Ciencias de la vida

Este es el lugar para ampliar sus conocimientos, capacidades de investigación y aplicaciones prácticas de la microscopía en diversos campos científicos. Aprenda a conseguir una visualización precisa, interpretación de imágenes y avances en la investigación. Encuentre información detallada sobre microscopía avanzada, técnicas de obtención de imágenes, preparación de muestras y análisis de imágenes. Los temas tratados incluyen la biología celular, la neurociencia y la investigación del cáncer, con especial atención a las aplicaciones e innovaciones de vanguardia.
LMD ionizer before & after cut.

Eliminating Electrostatic Interference in Laser Microdissection

Electrostatic charge in laser microdissection (LMD) causes two critical failures: samples stick to charged surfaces and are lost, or samples fly into adjacent wells and cause cross-contamination. We…

History, Developments and Trends of Microscopy in Cancer Research

Cancer is a global disease, with 18 million new cases diagnosed and 10 million cancer-related deaths worldwide in 2020. This burden is set to increase, with a projected increase in cases of ~55% by…

Overview of Fluorescent Dyes in terms of Applications and Properties

An introduction to commonly used fluorescent dyes and an overview of their characteristics are given in this article. Fluorescence microscopy is used for the study of specific cellular components with…

Researchers Insights: Microscopy in Cancer Research

Discover how imaging techniques are driving cancer research forward. In this issue, we present comprehensive multimodal studies using microscopy, as well as new directions in intraoperative cancer…
Zebrafish heart, DAPI (nuclei, blue), Tropomyosin (cardiomyocytes, red) and GFP (primordial cardiac layer, green). Courtesy of Anna Jazwinska, University of Fribourg, Switzerland.

A Guide to Fluorescence Microscopy

Fluorescence microscopy uses the ability of fluorophores, dyes, or fluorescent proteins to emit light of a specific wavelength after being excited with light of a shorter wavelength. Biomolecules can…
Foraminifera (Ammonia confertitesta) labeled with membrane-permeable calcein, high-pressure frozen in salt water using EM ICE. The sample was cryo-planed and targeted with the M205 on the Cryo-Fluo Enuity, then transferred under cryo conditions to the Cryo-Stellaris for widefield and confocal imaging, revealing details of the staining pattern. Image courtesy: David Evans, University of Southampton.

High-Pressure Freezing for Organoids: Cryo CLEM & FIB Lift Out

Master cryo EM workflow steps for challenging 3D samples: when to choose HPF vs. plunge freezing, reproducible blotting/ice control, contamination aware transfers, Cryo CLEM 3D targeting in organoids,…
Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imager with a 2x, 0.15 NA objective at 3.4x zoom was used to produce this 425 µm Z-stack (26 positions) which is presented here as an Extended Depth of Field (EDoF) projection. Images courtesy of Dr. F. Birey, Dr. S. Pasca laboratory, Palo Alto, CA.

Guide to Live-Cell Imaging

For a wide range of applications in various research fields of life science, live-cell imaging is an indispensable tool for visualizing cells in a state as close to in vivo, i.e. living and active, as…
Shown is the DMi8 inverted microscope which is used for life-science research.

Factors to Consider When Selecting a Research Microscope

An optical microscope is often one of the central devices in a life-science research lab. It can be used for various applications which shed light on many scientific questions. Thereby the…
Electron microscope (EM) image of a cross section of C. elegans (roundworm). Courtesy of T. Müller-Reichert, MPI-CBG, Dresden, Germany and K. McDonald, University of California, Berkeley, USA.

Brief Introduction to High-Pressure Freezing for Cryo-Fixation

Preparation of biological specimens for electron microscopy (EM) often requires cryo-fixation which does not introduce significant structural alterations of cellular constituents. A common method used…
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