Learn how to Remove Autofluorescence from your Confocal Images
Autofluorescence can significantly reduce what you can see in a confocal experiment. This article explores causes of autofluorescence as well as different ways to remove it, from simple media fixes to…
What is a Spectral Detector (SP Detector)?
The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP detector splits light into up to 5 spectral bands.…
Which Sensor is the Best for Confocal Imaging?
The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.
The Fundamentals and History of Fluorescence and Quantum Dots
At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and…
Introduction to Widefield Microscopy
This article gives an introduction to widefield microscopy, one of the most basic and commonly used microscopy techniques. It also shows the basic differences between widefield and confocal…
Photoactivatable, Photoconvertible, and Photoswitchable Fluorescent Proteins
Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…
Rodent and Small-Animal Surgery
Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.
Investigating Fruit Flies (Drosophila melanogaster)
Learn how to image and investigate Drosophila fruit fly model organisms efficiently with a microscope for developmental biology applications from this article.
Acousto Optics in True Confocal Spectral Microscope Systems
Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this…
