Optical properties

  • λ abs = 453 nm
  • ε max = 7.5×104 M -1 cm -1
  • λ fl = 506 nm
  • n fl = 70 %
  • τ fl = 5.0 ns
  • CF 260 = ε 260 /ε max = 1.09
  • CF 280 = ε 280 / ε max = 0.48

ATTO 465 is a novel fluorescent label derived from the well-known dye Acriflavin. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. In aqueous solution the dye shows a surprisingly large Stokes-shift of 55 nm. The dye shows intense and long-lived phosphorescence in solid matrix, in particular at low temperatures. ATTO 465 is moderately hydrophilic. The fluorescence can be excited efficiently in the range 420 - 465 nm.

ATTO 465

ATTO 465 is a novel fluorescent label derived from the well-known dye Acriflavin. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. In aqueous solution the dye shows a surprisingly large Stokes-shift of 55 nm. The dye shows intense and long-lived phosphorescence in solid matrix, in particular at low temperatures. ATTO 465 is moderately hydrophilic. The fluorescence can be excited efficiently in the range 420 - 465 nm.

Fields of Application

Life Science Research

Leica Microsystems’ life science research microscopes support the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and…
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