Optical properties

  • λ abs = 603 nm
  • ε max = 1.2×105 M -1 cm -1
  • λ fl = 626 nm
  • n fl = 85 %
  • τ fl = 3.9 ns
  • CF 260 = ε 260max = 0.22
  • CF 280 = ε 280 / ε max = 0.50

ATTO 594 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and excellent water solubility. Thus ATTO 594 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.

ATTO 594 is very hydrophilic and shows a very good water solubility. After coupling to a substrate the label carries a net electrical charge of -1. The fluorescence is excited most efficiently in the range 580 - 615 nm.

ATTO 594

ATTO 594 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and excellent water solubility. Thus ATTO 594 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH and many more.

ATTO 594 is very hydrophilic and shows a very good water solubility. After coupling to a substrate the label carries a net electrical charge of -1. The fluorescence is excited most efficiently in the range 580 - 615 nm.

Fields of Application

Life Science Research

Leica Microsystems’ life science research microscopes support the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and…
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