ATTO 665 DOPE Labeled Phospholipid 5 mg
Product No. AD-665-165
ATTO-Dye Labeled Phospholipids
In recent years the investigation of biological membranes, e.g. intracellular membranes of live cells, plasma membranes etc., has become a major subject of interest. As a result there is a growing demand for fluorescent dye-labeled lipids, in particular glycero-phospholipids, to be incorporated in biological membranes. ATTO-TEC offers a variety of glycero-phospholipids, carrying one or two fatty acid chains (lipophilic groups) and a phosphate ester residue (hydrophilic group).
ATTO-dye fluorescent phospholipids are labeled at the hydrophilic head group. After incorporation in, e.g., a plasma membrane (phospholipid bilayer) the fluorophore is located at the water/lipid interface of the membrane.
"ATTO-TEC is a wholly owned subsidiary of Leica Microsystems"
Currently we provide the phospholipids
- 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE),
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE),
- 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine (PPE)
- 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), and
- 1,2-dilauroyl-sn-glycero-3-phosphoethanolamine (DLPE)
labeled with ATTO-dyes.
ATTO 665
ATTO 665 is a new fluorescent label related to ATTO 647N.
Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications.
ATTO 665 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. ATTO 665 is a hydrophobic dye. The fluorescence is excited most efficiently in the range 640 - 675 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.
