ATTO 665

ATTO 665 is a new fluorescent label related to ATTO 647N.

Optical properties

λ _abs = 662nm
ε _max = 1.60×10⁵ M ^-1 cm ^-1
λ _fl = 680 nm
n _fl = 60 %
τ _fl = 2.9 ns
CF ₂₆₀ = ε ₂₆₀ /ε _max = 0.07
CF ₂₈₀ = ε ₂₈₀ / ε _max = 0.06

ATTO 665 is a new fluorescent label related to ATTO 647N.

Characteristic features of the label are strong absorption, extraordinarily high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. In common with most ATTO-TEC-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications.

ATTO 665 is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. ATTO 665 is a hydrophobic dye. The fluorescence is excited most efficiently in the range 640 - 675 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

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Modification

Unit of measure

ATTO 665 Biotin Fluorescent Label 5 mg

Product No. AD-665-75

ATTO 665 ist ein neuer Farbstoff vom Typ ATTO 647N.

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

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ATTO 665 Biotin Fluorescent Label 5 mg

Product No. AD-665-75

ATTO 665 ist ein neuer Farbstoff vom Typ ATTO 647N.

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

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ATTO 665 Carboxy Fluorescent Label 1 mg

Product No. AD-665-21

ATTO 665 ist ein neuer Farbstoff vom Typ ATTO 647N.

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

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ATTO 665 Carboxy Fluorescent Label 5 mg

Product No. AD-665-25

ATTO 665 ist ein neuer Farbstoff vom Typ ATTO 647N.

ATTO-TEC is a wholly owned subsidiary of Leica Microsystems

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ATTO 665 DOPE Labeled Phospholipid 1 mg

Product No. AD-665-161

ATTO-Dye Labeled Phospholipids

In recent years the investigation of biological membranes, e.g. intracellular membranes of live cells, plasma membranes etc., has become a major subject of interest. As a result there is a growing demand for fluorescent dye-labeled lipids, in particular glycero-phospholipids, to be incorporated in biological membranes. ATTO offers a variety of glycero-phospholipids, carrying one or two fatty acid chains (lipophilic groups) and a phosphate ester residue (hydrophilic group).

ATTO-dye fluorescent phospholipids are labeled at the hydrophilic head group. After incorporation in, e.g., a plasma membrane (phospholipid bilayer) the fluorophore is located at the water/lipid interface of the membrane.

"ATTO-TEC is a wholly owned subsidiary of Leica Microsystems"