Optical properties

  • λ abs = 681 nm
  • ε max = 1.25×105 M -1 cm -1
  • λ fl = 698 nm
  • n fl = 30 %
  • τ fl = 1.7 ns
  • CF 260 = ε 260 /ε max = 0.30
  • CF 280 = ε 280 / ε max = 0.17

ATTO 680 belongs together with ATTO 655 and ATTO 700 to a new generation of fluorescent labels.

Characteristic features of the label are strong absorption, high fluorescence quantum yield, good water solubility, and high thermal and photo-stability. ATTO 680 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 680 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.

The fluorescence is excited most efficiently in the range 645 - 695 nm. A suitable excitation source for the dye is the Krypton-Ion laser using the 676 nm line or a diode-laser emitting at 670 nm.

ATTO 680

ATTO 680 belongs together with ATTO 655 and ATTO 700 to a new generation of fluorescent labels.

Characteristic features of the label are strong absorption, high fluorescence quantum yield, good water solubility, and high thermal and photo-stability. ATTO 680 is a zwitterionic dye. After coupling to a substrate the dye moiety is electrically neutral. ATTO 680 is a strong electron acceptor. Its fluorescence is efficiently quenched by electron donors like guanine, tryptophan, etc.

The fluorescence is excited most efficiently in the range 645 - 695 nm. A suitable excitation source for the dye is the Krypton-Ion laser using the 676 nm line or a diode-laser emitting at 670 nm.

Fields of Application

Life Science Research

Leica Microsystems’ life science research microscopes support the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and…
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