Optical properties

  • λ abs = 566 nm
  • ε max = 1.2×105 M -1 cm -1
  • λ fl = 589 nm
  • n fl = 50 %
  • τ fl = 1.5 ns
  • CF 260 = ε 260max = 0.27
  • CF 280 = ε 280 / ε max = 0.13

ATTO Rho3B is a new fluorescent label related to the well-known dye Rhodamine B. The dye shows strong absorption and high thermal and photo-stability. The characteristic feature of the dye is its temperature depending fluorescence yield. The dye is moderately hydrophilic. ATTO Rho3B is a cationic dye.

After coupling to a substrate the dye carries a net electrical charge of +1. In solution its fluorescence can be excited efficiently in the range 545 - 575 nm.

ATTO Rho3B

ATTO Rho3B is a new fluorescent label related to the well-known dye Rhodamine B. The dye shows strong absorption and high thermal and photo-stability. The characteristic feature of the dye is its temperature depending fluorescence yield. The dye is moderately hydrophilic. ATTO Rho3B is a cationic dye.

After coupling to a substrate the dye carries a net electrical charge of +1. In solution its fluorescence can be excited efficiently in the range 545 - 575 nm.

Fields of Application

Life Science Research

Leica Microsystems’ life science research microscopes support the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and…
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